A5039091506- Circadian rhythm and melatonin
- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- Genetics, Aging, and Longevity in Model Organisms
- Light effects on plants
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- EEG and Brain-Computer Interfaces
- Health, Environment, Cognitive Aging
- Advanced Fluorescence Microscopy Techniques
- Neurobiology and Insect Physiology Research
- Sleep and related disorders
- Spaceflight effects on biology
- Mass Spectrometry Techniques and Applications
- Dietary Effects on Health
- RNA and protein synthesis mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Photoreceptor and optogenetics research
- Gene expression and cancer classification
- Neural dynamics and brain function
- thermodynamics and calorimetric analyses
- Greenhouse Technology and Climate Control
- Microtubule and mitosis dynamics
- Spectroscopy and Chemometric Analyses
- Dental Implant Techniques and Outcomes
Kobe University
2025
RIKEN Center for Biosystems Dynamics Research
2018-2025
Japan Agency for Medical Research and Development
2025
Kurume University
2024
Systems Biology Institute
2024
Osaka University
2022
Astellas Pharma (Japan)
2013
Hiroshima University
2013
Nagasaki University
2013
RIKEN Center for Computational Science
2007-2009
A striking feature of the circadian clock is its flexible yet robust response to various environmental conditions. To analyze biochemical processes underlying this flexible-yet-robust characteristic, we examined effects 1,260 pharmacologically active compounds in mouse and human cell lines. Compounds that markedly (>10 s.d.) lengthened period both lines, also it central tissues peripheral cells. Most inhibited casein kinase Iε (CKIε) or CKIδ phosphorylation PER2 protein. Manipulation...
The Drosophila circadian clock consists of integrated autoregulatory feedback loops, making the difficult to elucidate without comprehensively identifying network components in vivo. Previous studies have adopted genome-wide screening for clock-controlled genes using high-density oligonucleotide arrays that identified hundreds genes. In an attempt identify core among these candidates, we applied functional RNA interference (RNAi) system Here report identification novel gene candidates...
Sleep regulation involves interdependent signaling among specialized neurons in distributed brain regions. Although acetylcholine promotes wakefulness and rapid eye movement (REM) sleep, it is unclear whether the cholinergic pathway essential (i.e., absolutely required) for REM sleep because of redundancy from neural circuits to molecules. First, we demonstrate that synaptic inhibition TrkA+ causes a severe short-sleep phenotype reduction mostly attributable shortened duration dark phase....
The reduced sleep duration previously observed in Camk2b knockout mice revealed a role for Ca 2+ /calmodulin-dependent protein kinase II (CaMKII)β as sleep-promoting kinase. However, the underlying mechanism by which CaMKIIβ supports regulation is largely unknown. Here, we demonstrate that activation or inhibition of can increase decrease almost 2-fold, supporting core regulator mammals. Importantly, show this depends on activity CaMKIIβ. A mutant mimicking constitutive-active...
Aging affects emotional, cognitive, and social functions, increasing susceptibility to neuropsychiatric conditions. C57BL/6 mice are commonly used study aging mechanisms, yet differences between C57BL/6J C57BL/6N substrains remain underexplored. This compared aging-related behavioral changes in these substrains. reduced exploratory activity heightened anxiety C57BL/6J, but not C57BL/6N, mice. Conversely, novelty preference Male of both exhibited increased female urine sniffing with age....
<title>Abstract</title> Neurodegenerative diseases are characterized by progressive neuronal loss, yet subtle degeneration remains difficult to detect, hindering advancements in early diagnosis and intervention strategies. Here, we present a 3D, whole-brain, single-neuron atlas that aligns compares neurons at single-cell resolution, surpassing 2D spatial limitations precisely detect heterogeneous neurodegenerative lesions. Using the AppNL-G-F model of Alzheimer’s disease, found loss begins...
Abstract The homeostatic regulation of sleep is characterized by rebound after prolonged wakefulness, but the molecular and cellular mechanisms underlying this are still unknown. In study, we show that Ca 2+ /calmodulin-dependent protein kinase II (CaMKII)-dependent activity control parvalbumin (PV)-expressing cortical neurons involved in male mice. Prolonged wakefulness enhances PV-neuron activity. Chemogenetic suppression or activation PV inhibits induces sleep, implying dependent on...
Recent advancements in tissue clearing technologies have offered unparalleled opportunities for researchers to explore the whole mouse brain at cellular resolution. With expansion of this experimental technique, however, a scalable and easy-to-use computational tool is demand effectively analyze integrate whole-brain mapping datasets. To that end, here we present CUBIC-Cloud, cloud-based framework quantify, visualize, data. CUBIC-Cloud fully automated system where users can upload their...
Abstract Electroencephalogram (EEG) and electromyogram (EMG) are fundamental tools in sleep research. However, investigations into the statistical properties of rodent EEG/EMG signals sleep–wake cycle have been limited. The lack standard criteria defining stages forces researchers to rely on human expertise inspect EEG/EMG. recent increasing demand for analysing large‐scale long‐term data has overwhelming capabilities experts. In this study, we explored features EEG cycle. We found that...
We screened circadian-regulated genes in rat cartilage by using a DNA microarray analysis. In rib growth-plate cartilage, numerous showed statistically significant circadian mRNA expression under both 12:12 h light-dark and constant darkness conditions. Type II collagen aggrecan genes--along with several essential for post-translational modifications of aggrecan, including prolyl 4-hydroxylase 1, lysyl oxidase, oxidase-like 2 3'-phosphoadenosine 5'-phosphosulphate synthase 2--showed the same...
ABSTRACT Recent advancements in tissue clearing technologies have offered unparalleled opportunities for researchers to explore the whole mouse brain at cellular resolution. With expansion of this experimental technique, however, a scalable and easy-to-use computational tool is demand effectively analyze integrate whole-brain mapping datasets. To that end, here we present CUBIC-Cloud, cloud-based framework quantify, visualize data. CUBIC-Cloud fully automated system where users can upload...
Microfluidic devices are important platforms to culture and observe biological tissues. Compared with conventional setups, microfluidic have advantages in perfusion, including an enhanced delivery of nutrients gases However, explanted tissues can maintain their functions for only hours days devices, although observations desired weeks. The suprachiasmatic nucleus (SCN) is a brain region composed heterogeneous cells control the clock system through synchronizing individual this region....
Abstract The homeostatic regulation of sleep is characterized by rebound after prolonged wakefulness, but the molecular and cellular mechanisms underlying this are still unknown. We show here that CaMKII-dependent activity control parvalbumin (PV)-expressing cortical neurons involved in homeostasis regulation. Prolonged wakefulness enhances PV-neuron activity. Chemogenetic suppression or activation PV inhibits induces sleep, implying dependent on increased neurons. Furthermore, we discovered...
Many mammalian proteins have circadian cycles of production and degradation, many these rhythms are altered posttranscriptionally. We used ribosome profiling to examine posttranscriptional control by quantifying RNA translation in the liver over a 24-h period from circadian-entrained mice transferred constant darkness conditions comparing binding levels protein for 16 proteins. observed large differences compared levels, we delays between peak abundance. found extensive ribosomes upstream...
Abstract Understanding synaptic dynamics during the sleep-wake cycle is crucial yet remains controversial. The homeostasis hypothesis (SHY) suggests depression non-rapid eye movement (NREM) sleep, while other studies report potentiation or changes NREM sleep depending on activities in wakefulness. To find boundary conditions between these contradictory observations, we focused learning rules and firing patterns that contribute to dynamics. Using computational models, found under Hebbian...