A5103139363- Monoclonal and Polyclonal Antibodies Research
- Cancer Mechanisms and Therapy
- Hypothalamic control of reproductive hormones
- Chromatin Remodeling and Cancer
- Glycosylation and Glycoproteins Research
- Regulation of Appetite and Obesity
- Fibroblast Growth Factor Research
- Diabetes and associated disorders
- Trace Elements in Health
- Antimicrobial Peptides and Activities
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- Iron Metabolism and Disorders
- Protein Degradation and Inhibitors
- RNA and protein synthesis mechanisms
- Proteoglycans and glycosaminoglycans research
- Platelet Disorders and Treatments
- Hemoglobinopathies and Related Disorders
- RNA modifications and cancer
- Protein purification and stability
- Biochemical Analysis and Sensing Techniques
- Enzyme Production and Characterization
- Nuclear Receptors and Signaling
- interferon and immune responses
- Microbial Community Ecology and Physiology
St. Jude Children's Research Hospital
2024
Molecular Oncology (United States)
2024
The Royal Free Hospital
1998-2004
University College London
1998-2004
Babraham Institute
2003
Roland Hill (United Kingdom)
2001
University of Pennsylvania
2001
University of Colorado Health
2001
University of Nebraska–Lincoln
2001
OBJECTIVE The association of idiopathic hypogonadotrophic hypogonadism (IHH) with congenital olfactory deficit defines Kallmann's syndrome (KS). Although a small proportion IHH patients have been found to harbour defined genetic lesions, the basis most cases remains be elucidated. Genes currently recognized involved comprise KAL (associated X‐linked‐KS), GnRH receptor resistance therapy), DAX 1 adrenohypoplasia congenita) and three loci also associated obesity, leptin (OB), (DB) prohormone...
Human complement receptor type 2 (CR2, CD21) is a cell surface that binds three distinct ligands (complement C3d, Epstein−Barr virus gp350/220, and the low-affinity IgE CD23) via N-terminal two of fifteen or sixteen short consensus/complement repeat (SCR) domains. Here, we report biophysical studies CR2 SCR 1-2 domain binding to its ligand C3dg. Two recombinant forms containing 1-15 domains were expressed in high yield Pichia pastoris baculovirus, respectively. Circular dichroism...
Genes encoding subunits of SWI/SNF (BAF) chromatin remodeling complexes are mutated in nearly 25% cancers. To gain insight into the mechanisms by which mutations drive cancer, we contributed ten rhabdoid tumor (RT) cell lines mutant for subunit SMARCB1 to a genome-scale CRISPR–Cas9 depletion screen performed across 896 lines. We identify PHF6 as specifically essential RT survival and demonstrate that dependency on Phf6 extends Smarcb1-deficient cancers vivo. As either or can cause...
Anosmin-1, the gene product of KAL gene, is implicated in pathogenesis X-linked Kallmann's syndrome. Anosmin-1 protein expression restricted to basement membrane and interstitial matrix tissues affected this syndrome during development. The anosmin-1 sequence indicates an N-terminal cysteine-rich domain, a whey acidic (WAP) four fibronectin type III (FnIII) domains C-terminal histidine-rich region, shows similarity with cell-adhesion molecules, such as neural molecule, TAG-1 L1. We...
Anosmin-1, the gene product of KAL gene, is implicated in pathogenesis X-linked Kallmann's syndrome. Anosmin-1 protein expression restricted to basement membrane and interstitial matrix tissues affected this syndrome during development. The anosmin-1 sequence indicates an N-terminal cysteine-rich domain, a whey acidic (WAP) four fibronectin type III (FnIII) domains C-terminal histidine-rich region, shows similarity with cell-adhesion molecules, such as neural molecule, TAG-1 L1. We...
Abstract The I domain of integrin αE was modeled on the crystal structure that in CD11b and mutated to produce an open (high affinity) or closed (low conformation. K562 transfectants expressing mutant wild‐type β7 were tested for adhesion E‐cadherin‐Fc. Downward displacement C terminus αI with a disulfide bridge enhanced Mn 2+ dependency. Adhesion greatly exceeded observed using wild type under similar conditions. gave poor which improved by PMA‐induced clustering. Blocking function βI...