A5101963292- CRISPR and Genetic Engineering
- Biomarkers in Disease Mechanisms
- Clusterin in disease pathology
- Medicinal Plants and Bioactive Compounds
- Glycogen Storage Diseases and Myoclonus
- Bladder and Urothelial Cancer Treatments
- Cancer, Hypoxia, and Metabolism
- Esophageal and GI Pathology
- RNA modifications and cancer
- Genomic variations and chromosomal abnormalities
- Renal and related cancers
- Mosquito-borne diseases and control
- Bone and Dental Protein Studies
- Glycosylation and Glycoproteins Research
- Protein Tyrosine Phosphatases
- Cytomegalovirus and herpesvirus research
- Microbial Natural Products and Biosynthesis
- Genetics and Neurodevelopmental Disorders
- Traditional Chinese Medicine Analysis
- Cardiac Valve Diseases and Treatments
- Advanced biosensing and bioanalysis techniques
- Vascular Anomalies and Treatments
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- Natural Products and Biological Research
Beijing Chaoyang Emergency Medical Center
2024
Second Military Medical University
2024
Capital Medical University
2022-2024
Shanghai Pudong New Area Gongli Hospital
2024
Sichuan University
2019-2024
Suizhou Central Hospital
2023-2024
Shanxi University
2024
Beijing Chao-Yang Hospital
2022
State Key Laboratory of Biotherapy
2019
Zhongshan Hospital
2016
The purpose of this study was to evaluate the utility potential serum biomarkers for acute aortic dissection (AAD) that were identified by isobaric Tags Relative and Absolute Quantitation (iTRAQ) approaches. Serum samples from 20 AAD patients healthy volunteers analyzed using iTRAQ technology. Protein validation performed 120 with chest pain. A total 355 proteins approach; 164 reached strict quantitative standard, 125 increased or decreased more than 1.2-fold (64 61 up- downregulated,...
As the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12a (previously known as Cpf1) system cleaves double-stranded DNA and produces a sticky end, it could serve useful tool for assembly/editing. To broaden its application, variety of engineered FnCas12a proteins are generated with expanded protospacer adjacent motif (PAM) requirements. Two variants (FnCas12a-EP15 EP16) increased targeting range by approximately fourfold. They can efficiently recognize broad PAM...
TBX6 encodes transcription-factor box 6, a transcription factor critical to paraxial mesoderm segmentation and somitogenesis during embryonic development. haploinsufficiency is believed drive the skeletal kidney phenotypes associated with 16p11.2 deletion syndrome. Heterozygous biallelic variants in are vertebral rib malformations (TBX6-associated congenital scoliosis) spondylocostal dysostosis, heterozygous increased risk of genitourinary tract malformations. Combined individuals harboring...
The multifunctional proteins of class 2 CRISPR systems such as Cas9, have been employed to activate cryptic biosynthetic gene clusters (BGCs) in Streptomyces, which represent a large and hidden reservoir natural products. However, approaches are not applicable most Streptomyces strains with reasons be comprehended. Inspired by the prevalence 1 subtype especially type I-E system here we report development into series transcriptional regulation tools. We further demonstrate effectiveness...
Salidroside (Sal) contains anti-carcinogenic, anti-hypoxic, and anti-inflammatory pharmacological activities. However, its underlying anti-breast cancer mechanisms have been only incompletely elucidated. Hence, this protocol intended to decode the potential of Sal in regulating PI3K-AKT-HIF-1α-FoxO1 pathway malignant proliferation human breast MCF-7 cells. First, activity against was evaluated by CCK-8 cell scratch assays. Moreover, resistance cells measured migration Matrigel invasion For...
Background Congenital scoliosis and congenital anomalies of the kidney urinary tract are distinct genetic disorders with differing clinical manifestations. Clinically, their coexistence is not rare, but etiologies these complex diseases remain largely unknown, especially shared basis. Methods We sequenced genomes 40 individuals diagnosed both CS CAKUT, alongside 2,764 controls from a Chinese Han population cohort. Our analyses encompassed gene-based pathway-based weighted rare variant...
In the past few decades, researchers have made promising progress, including development of immune checkpoint inhibitors (ICIs) in therapy bladder cancer (BLCA). Existing studies mainly focus on single but lack relevant gene expression profiles multiple checkpoints.
Background The manifestation of severe sepsis is sepsis-induced myocardial dysfunction (SIMD), which the chief cause elevated death in patients. Sepsis mainly attacks heart, and effective switch inflammatory cascades prodigious implication relieving sepsis-allied impairments. Natural antioxidants with anti-inflammatory antiapoptotic properties are promptly required as typical anti-septic agents. Purpose: Morus alba root bark contains a flavonoid sanggenol L (SL), exhibits antioxidant,...
Salidroside (Sal) contains anti-carcinogenic, anti-hypoxic, and anti-inflammatory pharmacological activities. However, its underlying anti-breast cancer mechanisms have been only incompletely elucidated. Hence, this protocol intended to decode the potential of Sal in regulating PI3K-AKT-HIF-1α-FoxO1 pathway malignant proliferation human breast MCF-7 cells. First, activity against was evaluated by CCK-8 cell scratch assays. Moreover, resistance cells measured migration Matrigel invasion For...
Abstract Introduction: SN-38 is a critical chemotherapeutic agent active against variety of solid tumors including advanced colorectal cancer. Clinical use hampered by its poor solubility in pharmaceutically acceptable solvents. Although irinotecan, the only approved prodrug SN-38, widely used, efficacy limited low conversion to as well hematologic and gastrointestinal dose-limiting toxicities. In an attempt improve water solubility, antitumor activity safety profiles we synthesized soluble...