A5009978389- Heart Rate Variability and Autonomic Control
- Photoacoustic and Ultrasonic Imaging
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Thermography and Photoacoustic Techniques
- Stress Responses and Cortisol
- Optical Imaging and Spectroscopy Techniques
- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Rheumatoid Arthritis Research and Therapies
- Cardiac tumors and thrombi
- AI in cancer detection
- Osteoarthritis Treatment and Mechanisms
- Lower Extremity Biomechanics and Pathologies
- Autoimmune and Inflammatory Disorders Research
- Prostate Cancer Treatment and Research
- Angiogenesis and VEGF in Cancer
- Cell Image Analysis Techniques
- Extracellular vesicles in disease
- Adenosine and Purinergic Signaling
- Optical Coherence Tomography Applications
- Ocular Oncology and Treatments
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Radiopharmaceutical Chemistry and Applications
- Advanced Fluorescence Microscopy Techniques
Goethe University Frankfurt
2021-2024
University Hospital Frankfurt
2021-2024
Orthopaedic University Hospital Friedrichsheim
2021-2024
Max Planck Institute for Molecular Biomedicine
2022
Washington University in St. Louis
2011-2017
Although our understanding of the molecular regulation adult neovascularization has advanced tremendously, vascular-targeted therapies for tissue ischemia remain suboptimal. The master regulatory transcription factors hypoxia-inducible factor (HIF) family are attractive therapeutic targets because they coordinately up-regulate multiple genes controlling neovascularization. Here, we used an inducible model epithelial HIF-1 activation, TetON-HIF-1 mouse, to test requirement VEGF in mediated...
With the increasing use of small animals for human disease studies, small-animal whole-body molecular imaging plays an important role in biomedical research. Currently, none existing modalities can provide both anatomical and glucose information, leading to higher costs building dual-modality systems. Even with image co-registration, spatial resolution modality is not improved. Utilizing a ring-shaped confocal photoacoustic computed tomography system, we demonstrate, first time, that anatomy...
Abstract Background Osteoarthritis (OA) is a chronic degenerative joint disease causing limited mobility and pain, with no curative treatment available. Recent in vivo studies suggested autonomic alterations during OA progression patients, yet clinical evidence scarce. Therefore, tone was analyzed patients via heart rate variability (HRV) measurements. Methods Time-domain (SDRR, RMSSD, pRR50) frequency-domain (LF, HF, LF/HF) HRV indices were determined to quantify sympathetic parasympathetic...
The importance of neovascularization for primary and metastatic tumor growth fostered numerous clinical trials angiogenesis inhibitors either alone or in combination with conventional antineoplastic therapies. One challenge the use molecularly targeted agents has been disconnection between size reduction biologic behavior, when drug is efficacious resistance emerges. Here, we report synthesis characterization 64Cu-NOTA-bevacizumab as a PET imaging agent intratumoral VEGF content vivo. avidly...
Exogenous adenosine and its metabolite inosine exert anti-inflammatory effects in synoviocytes of osteoarthritis (OA) rheumatoid arthritis (RA) patients. We analyzed whether these cells are able to synthesize adenosine/inosine which receptors (ARs) contribute effects. The functionality synthesizing enzymes ARs was tested using agonists/antagonists. Both OA RA expressed CD39 (converts ATP AMP), CD73 AMP adenosine), ADA inosine), ENT1/2 (adenosine transporters), all AR subtypes (A1, A2A, A2B...
Abstract In recent years, 3D cell culture has been gaining a more widespread following across many fields of biology. Tissue clearing enables optical analysis intact samples and investigation molecular structural mechanisms by homogenizing the refractive indices tissues to make them nearly transparent. Here, we describe quantify that common solutions including benzyl alcohol/benzyl benzoate (BABB), PEG-associated solvent system (PEGASOS), immunolabeling-enabled imaging solvent-cleared organs...
Collaborative manual image analysis by multiple experts in different locations is an essential workflow biomedical science. However, sharing the images and writing down results hand or merging from separate spreadsheets can be error-prone. Moreover, blinding anonymization are to address subjectivity bias. Here, we propose a new for collaborative using lightweight online tool named Tyche. The allows access via temporarily valid URLs analyze them blind random order inside web browser with...
Angiogenesis in a tumor region creates arteriovenous (AV) shunts that cause an abnormal venous blood oxygen saturation (sO2) distribution. Here, we applied optical-resolution photoacoustic microscopy to study the AV shunting vivo. First, built phantom image sO2 distribution vessel containing converged flows from two upstream vessels with different values. The experiment showed maintained clear boundary for hundreds of seconds, which is consistent our theoretical analysis using diffusion...
Purpose Risk factors for the development of pain in context knee osteoarthritis (KOA) remain unclear. Radiological findings often do not correlate with clinical findings, so other pathomechanisms and perception must play a role. The purpose this study is to investigate correlation increased sympathetic nervous system (SNS) activity (measured by subjective objective chronic stress parameters) KOA severity, patellofemoral malalignment, pain. Methods 47 patients were assessed. measurements...
// Zhi Hong Lu 1, 2 , Sergey Kaliberov 2, 3, 4 Rebecca E. Sohn Lyudmila Kaliberova Yingqiu Du Julie L. Prior 5 Daniel J. Leib 6 Anne Chauchereau 7 Jennifer K. Sehn 8 David T. Curiel Jeffrey M. Arbeit 1 Urology Division and Department of Surgery, Washington University in St. Louis School Medicine, Louis, MO 63110, USA Siteman Cancer Center, 3 Biologic Therapeutics Radiation Oncology, Radiology, Orthopedic Prostate Group, INSERM U981, Gustave Roussy, Villejuif, F-94805, France Anatomic...
New approaches targeting metastatic neovasculature are needed. Payload capacity, cellular transduction efficiency, and first-pass uptake following systemic vector administration, motivates persistent interest in tumor vascular endothelial cell (EC) adenoviral (Ad) targeting. While EC transductional transcriptional has been accomplished, administration of limited clinical utility, lack tumor-wide expression quantification, failure to address avid liver sequestration, challenged prior work....
We propose using noninvasive longitudinal optical-resolution photoacoustic microscopy (L-ORPAM) to quantify blood flow flux, oxygen saturation (sO<sub>2</sub>), and thereby the metabolic rate of (MRO<sub>2</sub>), for a renal tumor model in same mouse over weeks months. Experiments showed that sO2 difference between artery vein decreased greatly due arteriovenous shunting effect during growth. Moreover, hypermetabolism was exhibited by an increase MRO<sub>2</sub>.
Osteoarthritis (OA) is the most common degenerative joint disorder. Its multifactorial etiology includes age, sex, overloading, genetic or nervous influences. In particular, autonomic system increasingly gaining in importance. two branches, sympathetic (SNS) and parasympathetic system, are well-balanced under healthy conditions. OA patients seem to be prone an imbalance therefore, we analyzed their status. More than 200 participants including with early late stage knee (before 1 year after...
Introduction Recent studies suggested that the progression of osteoarthritis (OA), a chronic degenerative joint disease, may be affected by autonomic nervous system (ANS). Under healthy conditions, sympathetic (SNS) and parasympathetic (PNS) branches ANS are well coordinated to maintain homeostasis. However, pathological conditions frequently associated with disturbance this fine-tuned balance. Therefore, we analyzed whether an dysfunction occurs in OA patients. Method 225 participants...
We report photoacoustic microscopy (PAM) of arteriovenous (AV) shunts in early stage tumors vivo, and develop a pattern recognition framework for computerized tumor detection. Here, using high-resolution microscope, we implement new blood oxygenation (sO<sub>2</sub>)-based disease marker induced by the AV shunt effect angiogenesis. discovered striking biological phenomenon: There can be two dramatically different sO<sub>2</sub> values bloodstreams flowing side-by-side single vessel. By...
We developed multi-contrast photoacoustic microscopy (PAM) for in vivo anatomical, functional, metabolic, and molecular imaging. This technical innovation enables comprehensive understanding of the tumor microenvironment. With PAM, we longitudinally determined vascular anatomy, blood flow, oxygen saturation hemoglobin, extraction fraction.