A5034277088- Parasitic infections in humans and animals
- Parasitic Infections and Diagnostics
- Congenital Anomalies and Fetal Surgery
- Parasite Biology and Host Interactions
- Parasites and Host Interactions
- Toxoplasma gondii Research Studies
- Vector-borne infectious diseases
- Amoebic Infections and Treatments
- Vector-Borne Animal Diseases
- Toxin Mechanisms and Immunotoxins
- Helminth infection and control
- Urological Disorders and Treatments
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Climate Change, Adaptation, Migration
- Prion Diseases and Protein Misfolding
- Biosensors and Analytical Detection
- Genomics and Phylogenetic Studies
- Salmonella and Campylobacter epidemiology
- Entomopathogenic Microorganisms in Pest Control
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Vectors
- Insect and Pesticide Research
- Viral gastroenteritis research and epidemiology
- Machine Learning in Bioinformatics
Asahikawa Medical University
2013-2025
Asahikawa Medical College Hospital
2003-2017
Ministry of Scientific Research and Innovation
2016
Tokyo Metropolitan Bokutoh Hospital
2013
Mie University
2013
Cambridge University Press
2013
University Hospital Ulm
2010
Institute of Medical Microbiology and Hygiene
2010
University of Würzburg
2009
Sichuan Center for Disease Control and Prevention
2009
ABSTRACT Multiplex PCR was established for differential diagnosis of taeniasis and cysticercosis, including their causative agents. For identification the parasites, multiplex with cytochrome c oxidase subunit 1 gene yielded evident products unique Taenia saginata asiatica American/African Asian genotypes solium molecular sizes 827, 269, 720, 984 bp, respectively. In PCR-based detection tapeworm carriers using fecal samples, diagnostic markers were detected from 7 14 4 9 T. Guatemala...
Genetic polymorphism was determined among 13 isolates of Taenia solium from various regions using PCR-amplified sequences 2 mitochondrial genes: cytochrome c oxidase subunit 1 and b . The phylogenies obtained were similar to each other regardless the genes examined. Asia (China, Thailand, Irian Jaya India) formed a single cluster, whereas Latin America (Mexico, Peru, Ecuador, Bolivia Brazil) combined with those Africa (Tanzania, Mozambique Cameroon) form an additional cluster. These results...
ABSTRACT Rapid detection and differentiation of Taenia species are required for the control prevention taeniasis cysticercosis in areas where these diseases endemic. Because lower sensitivity specificity conventional diagnosis based on microscopical examination, molecular tools more reliable differential diseases. In this study, we developed evaluated a loop-mediated isothermal amplification (LAMP) assay infections with cathepsin L-like cysteine peptidase (clp) cytochrome c oxidase subunit 1...
SUMMARY In Russia, both alveolar and cystic echinococcoses are endemic. This study aimed to identify the aetiological agents of diseases investigate distribution each Echinococcus species in Russia. A total 75 specimens were collected from 14 host 2010 2012. Based on mitochondrial DNA sequences, they identified as granulosus sensu stricto (s.s.), E. canadensis multilocularis. s.s. was confirmed European Russia Altai region. Three genotypes, G6, G8 G10 detected Yakutia. G6 also found Four...
Liver transplantation (LT) is currently contraindicated in patients with residual or metastatic alveolar echinococcosis (AE) lesions. We evaluated the long-term course of such who underwent LT and were subsequently treated benzimidazoles. Clinical, imaging, serological, therapeutic data collected from 5 residual/recurrent AE lesions survived for more than 15 years. Since 2004, [(18) F]-2-fluoro-2-deoxyglucose (FDG)-positron emission tomography (PET) images available, levels serum antibodies...
ABSTRACT We compared the performance of loop-mediated isothermal amplification (LAMP) with that a multiplex PCR method for differential detection human Taenia parasites in fecal specimens from taeniasis patients. The LAMP method, no false positives, showed higher sensitivity (88.4%) than (37.2%). Thus, it is expected has high value molecular diagnosis taeniasis.
ABSTRACT Neurocysticercosis (NCC) caused by infection with the larvae of Taenia solium is an important cause neurological disease worldwide. In order to establish enzyme-linked immunosorbent assay (ELISA) for this using recombinant proteins, we carried out molecular cloning and identified four candidates as diagnostic antigens (designated Ag1, Ag1V1, Ag2, Ag2V1). Except Ag2V1, these clones could encode a 7-kDa polypeptide, Ag2V1 10-kDa polypeptide. All were very similar. proteins...
ABSTRACT The use of tick vaccines in mammalian hosts has been shown to be the most promising alternative control method current acaricides, which suffers from a number limitations. However, success this is dependent on identification, cloning, and vitro expression molecules involved mediation key physiological roles with respect biological as vector pest. We have sequenced characterized Haemaphysalis longicornis salivary gland-associated cDNA coding for 29-kDa extracellular matrix-like...
Background Human cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus, with liver as most frequently affected organ, is known to be highly endemic in Tibetan communities northwest Sichuan Province. Antiparasitic treatment albendazole remains primary choice for great majority patients this resource-poor remote area, though surgery common approach CE therapy that has potential remove cysts and lead complete cure. The current prospective study aimed assess...
Most part of Southeast Asia is considered endemic for human-infecting Taenia tapeworms; solium, T. saginata, and asiatica. However, until now there was no report the occurrence human cases asiatica in Lao PDR. This study, conducted Savannakhet Province, PDR, microscopically examined a total 470 fecal samples by Kato Katz method found 86% people harboring at least one helminth. Hookworms were detected 56% besides Opisthorchis like eggs (42%), Trichuris trichiura (27%), Ascaris spp. (14%),...
ABSTRACT Schistosomiasis, primarily caused by Schistosoma japonicum (Sj) in Asia, remains a major health concern the Philippines, affecting 12.4 million people and causing symptoms like fever, abdominal pain, hepatosplenomegaly. Chronic disease leads to stunting children, reinfection persists despite efforts reduce morbidity. Current strategies focused on mass drug administration (MDA) sporadic snail surveys, leaving gaps monitoring mitigating schistosomiasis transmission environment. To...
Abstract Serology (ELISA and immunoblot) using native glycoproteins, affinity purified a recombinant antigen is known to be highly specific Taenia solium cysticercosis in humans pigs. These techniques were applied for dogs the endemic area of Papua (Irian Jaya), Indonesia. Analysis dog sera by both ELISA immunoblot revealed 7 64 positive. Examination two sero-positive cysticerci T. brain heart these dogs. Mitochondrial DNA analysis confirmed that they same as previously from pigs biopsies...
ABSTRACT The Echinococcus multilocularis protein Em18 is one of the most promising antigens for use in serodiagnosis alveolar echinococcosis human patients. Here we identify an antigenic relationship between and a 65-kDa immunodominant E. surface previously identified as either EM10 or EmII/3. NH 2 -terminal sequence native was determined, revealing it to be fragment EM10. Experiments were undertaken investigate effect proteinase inhibitors on degradation crude extracts protoscoleces. found...
Abstract We confirmed sympatric occurrence of Taenia solium, T. saginata, and asiatica in western Thailand. DNA analysis morphologically identified a dual infection with indicated it was asiatica. To our knowledge, this report is the first from
We compared four enzyme-linked immunosorbent assays (ELISAs) with 172 serum samples from 28 patients alveolar echinococcosis in different clinical stages according to the World Health Organization–PNM (P = parasitic mass liver, N involvement of neighboring organs, M metastasis) staging system. The sequential antibody responses against Em 2plus , Em10, and Em18 antigens, a crude antigen extract were measured cohorts resected unresected lesions. Antibody levels all correlated PNM stage before...