A5027005779- Polyomavirus and related diseases
- Antenna Design and Analysis
- Bacteriophages and microbial interactions
- Parvovirus B19 Infection Studies
- Virus-based gene therapy research
- CAR-T cell therapy research
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- Biomedical and Engineering Education
- Animal Virus Infections Studies
- Cervical Cancer and HPV Research
- Immunotherapy and Immune Responses
- Energy Harvesting in Wireless Networks
- Science, Research, and Medicine
Memorial Sloan Kettering Cancer Center
2024
Yale University
2011-2016
National Cancer Institute
2013-2015
Center for Cancer Research
2013-2015
ABSTRACT Simian virus 40 (SV40) is a nonenveloped DNA that traffics through the endoplasmic reticulum (ER) en route to nucleus, but mechanisms of capsid disassembly and ER exit are poorly understood. We conducted an unbiased RNA interference screen identify cellular genes required for SV40 infection. infection was specifically inhibited by up 50-fold knockdown four different DNAJ molecular cochaperones or inhibition BiP, Hsp70 partner DNAJB11. These proteins were not initiation disassembly,...
BK polyomavirus (BKV) causes significant urinary tract pathogenesis in immunosuppressed individuals, including kidney and bone marrow transplant recipients. It is currently unclear whether BKV-neutralizing antibodies can moderate or prevent BKV disease. We developed reporter pseudoviruses based on seven divergent isolates performed neutralization assays sera from healthy human subjects. The results demonstrate that genotypes I, II, III, IV are fully distinct serotypes. While nearly all...
Polyomaviruses are nonenveloped viruses with capsids composed primarily of 72 pentamers the viral VP1 protein, which forms outer shell capsid and binds to cell surface oligosaccharide receptors. Highly conserved proteins from closely related polyomaviruses recognize different oligosaccharides. To determine whether amino acid changes restricted binding site sufficient receptor specificity how in usage affect tropism, we studied primate polyomavirus simian virus 40 (SV40), uses ganglioside GM1...
ABSTRACT Simian virus 40 (SV40), a polyomavirus that has served as an important model to understand many aspects of biology, induces dramatic cytoplasmic vacuolization late during productive infection monkey host cells. Although this activity led the discovery in 1960, mechanism is still not known. Pentamers major SV40 capsid protein VP1 bind ganglioside GM1, which serves cellular receptor for virus. In report, we show binding cell surface GM1 plays key role infection-induced vacuolization....