Pharmacokinetics and biodistribution studies are essential for characterizing fluorescent agents in vivo . However, few simple methods based on fluorescence imaging available that account tissue optical properties sample volume differences. We describe a method simultaneously quantifying mean intensity of whole blood homogenized tissues glass capillary tubes two agents, ABY-029 IRDye 680LT, using wide-field tissue-specific calibration curves. All curves demonstrated high degree linearity...

SignificancePaired-agent fluorescent molecular imaging approaches involve co-administration of a control (untargeted) agent with molecularly targeted to account for non-specific effects and quantify binding potential (BP)—a parameter proportional the concentration biomolecule. Accurate BP estimation often requires correction differences in plasma input functions (PIFs).AimWe provide simulation-based evaluation whether dual-channel pulse dye densitometry (PDD) can be used measure PIFs...

Pancreatic ductal adenocarcinomas (PDACs) are often treatment resistant, and as such widefield imaging methods for the evaluation of ECM composition needed would be high value to drug design strategies. Here we present a method measure relative abundance diffracting components in PDAC samples alongside penetration images. Orthotopic mouse xenografts grown assessment well is done using co-registration scanning x-ray diffraction (XRD) EGFR-specific fluorescent Preliminary data suggests...

SignificanceSurgical excision is the main treatment for solid tumors in oral squamous cell carcinomas, where wide local (achieving a healthy tissue margin of >5 mm around excised tumor) goal as it results reduced recurrence rates and improved overall survival.AimNo clinical methods are available to assess complete surgical intraoperatively while patient still on operating table; recent intraoperative back-bench fluorescence-guided surgery approaches have shown promise detecting "positive"...

In the United States, annual incidence of oral squamous cell carcinoma (OSCC) exceeds 50,000 cases. Primary tumor resection remains first line treatment in these patients yet follow up neck dissection and chemoradiation may be indicated if cancer has spread to draining lymph nodes. There is a push minimize morbidity from by sentinel node biopsy, where only node(s) primary are excised evaluated for spread. However, with current pathology methods, results not available surgeons until have been...

Post-operative assessment of resected tumor margins is critical to ensure the entirety malignant tissue has been removed from a patient. Microscopic post-excision current gold standard, however long wait times for proper specimen evaluation limit surgeon's ability be certain they obtained clear margins. To address this need, fluorescence-guided surgery approaches are under development that can yield molecular contrast between healthy and tissues intraoperatively. In head neck cancer...

Pancreatic ductal adenocarcinoma continues to be one of the most lethal cancers today with an abysmal ~8% 5-year survival rate that has remained relatively constant over time. This is thought largely due desmoplastic stroma in extracellular matrix these tumor types, inhibiting both penetration as well target engagement treatments. Here we present a methodology for evaluating monoclonal antibody's drug presence remodeling using paired-agent imaging principles and subcutaneous mouse model.

Accurate characterization of tissue biodistribution (BD) is essential for the development new drug and imaging agents. Recently, a novel method to quantify tissue-specific BD fluorescent agent has been developed characterized. A subsequent study, using this method, was performed in mice with subcutaneous tumor xenograft head neck squamous cell carcinoma (NHSCC). Here we utilized same characterize two agents, ABY-029 IRDye 680LT mouse orthotopic HNSCC model. Tissue-specific fluorescence...

Surgical excision is the primary treatment for solid tumors in oral squamous cell carcinomas, where achieving a healthy tissue margin of >5 mm goal. However, current clinical methods assessing surgical margins cannot provide assessment whole intraoperatively (while patient still on operating table) and while recent intraoperative fluorescence-guided surgery approaches have shown promise detected "positive" inadequate (<1 mm), they had limited success detection "close" (1-5 patients injected...

Cancer continues to be a significant global health issue in the 21st century, presenting substantial risks and well-being of individuals worldwide. Although there have been improvements comprehending molecular processes disease creating treatments that specifically target it, considerable portion patients continue encounter difficulties attaining favorable results. Conventional two-dimensional (2D) cell cultures extensively used cancer research. However, their inability accurately mimic...

Oral squamous cell carcinomas represent a significant number of cancers diagnosed globally. Of these cancers, surgical resection the primary tumor is standard treatment. Conventional methods assessing completeness are time-consuming, laborious, and cannot be used to evaluate entire margin resected tumor. As such, widefield fluorescence molecular imaging being explored as an intraoperative technique guide resections. The widely single-view, wide aperture techniques have had high success in...

Pancreatic ductal adenocarcinomas (PDACs) are often treatment resistant, and as such widefield imaging methods for the evaluation of ECM composition needed. Here we present a method to measure relative abundance diffracting components in PDAC samples alongside drug penetration images. Orthotopic mouse xenografts grown assessment well is done using co-registration scanning x-ray diffraction (XRD) EGFR-specific fluorescent Preliminary data suggests strongly negative correlation between large...

Abstract Background As mammography X‐ray imaging technologies advance and provide elevated contrast in soft tissues, a need has developed for reliable phantoms use system design component calibration. In advanced modalities such as refraction‐based methods, it is critical that capture the biological details seen clinical precancerous cancerous cases while minimizing artifacts may be caused due to phantom production. This work presents fabrication of breast tissue from cadaveric suitable both...

An increasing number of cancer surgery protocols are including sentinel lymph node biopsies on the day resection to stage for non-palpable spread through tumor draining nodes. The challenge is that often a tumor-positive will make it necessary perform an enhanced lymphatic network, and if processing not completed within timeframe surgery, then patients may have be called back additional or undergo amplified chemo radiation therapy. Our group working rapid staining fluorescence tomography...

In head and neck squamous cell carcinoma surgical cases, rapid detection of lymph node metastases is needed. Here we present a methodology for assessment in 1 cm nodes with &gt; 99% sensitivity.

Many head and neck squamous cell carcinoma surgical patients are left with residual tumor post excision surgery (inadequate margins). We present an imaging strategy capable of rapid margin status assessment to reduce overall burden.

In Head and Neck Squamous Cell Carcinomas (HNSCC), positive margins after surgical resection dominate the clinical outcome of patients. Between 15-30 precent all HNSCC surgeries result in a margin (&gt;5mm) that requires postoperative chemo-radiation, radiotherapy, and/or revision surgeries. To minimize local recurrence, assessment is typically performed through pathology departments; however, this technique often takes more than 24 hours to process only small fraction total evaluated. A...

A paired-agent fluorescent molecular imaging strategy is presented as a method to measure drug target engagement in whole tumor imaging. The protocol involves dynamic of pair targeted and control agents prior following antibody therapy. Simulations demonstrated that "drug engagement" can be estimated within 15%-error over wide range physiology (blood flow, vascular permeability, density) characteristics (affinity, binding rates). Experimental results the first vivo detection site barrier,...

The status of lymph node is considered a critical prognostic factor for staging and guiding the future adjuvant treatment in many cancer types. estimation undetected micrometastases (0.2-2mm diameter) by conventional pathology was around 30-60% cases which has created demand development more fast accurate approaches. In response, paired-agent imaging approach presented employing control agent to allow rapid, quantitative mapping microscopic cells nodes guide sectioning. To identify most...