A5001669997- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Insect and Arachnid Ecology and Behavior
- Photoreceptor and optogenetics research
- Plant and Biological Electrophysiology Studies
- Protein Structure and Dynamics
- Mitochondrial Function and Pathology
- Animal Behavior and Reproduction
- Neuroscience and Neuropharmacology Research
- Endoplasmic Reticulum Stress and Disease
Freie Universität Berlin
2018-2025
At presynaptic active zones (AZs), conserved scaffold protein architectures control synaptic vesicle (SV) release by defining the nanoscale distribution and density of voltage-gated Ca 2+ channels (VGCCs). While AZs can potentiate SV in minutes range, we lack an understanding how AZ components VGCCs engage into potentiation. We here establish dynamic, intravital single-molecule imaging endogenously tagged proteins at Drosophila undergoing homeostatic During potentiation, numbers α1 VGCC...
High-throughput electron microscopy has started to reveal synaptic connectivity maps of single circuits and whole brain regions, for example, in the Drosophila olfactory system. However, efficacy, timing, frequency tuning vesicle release are also highly diversified across synapses. These features critically depend on nanometer-scale coupling distance between voltage-gated Ca2+ channels (VGCCs) machinery. Combining light super resolution with vivo electrophysiology, we show here that two...
At presynaptic active zones, arrays of large conserved scaffold proteins mediate fast and temporally precise release synaptic vesicles (SVs). SV sites could be identified by clusters Munc13, which allow SVs to dock in defined nanoscale relation Ca2+ channels. We here show Drosophila that RIM-binding protein (RIM-BP) connects physically functionally the ELKS family Bruchpilot (BRP)-based engaged recruitment. The RIM-BP N-terminal domain, while dispensable for site organization, was crucial...
Abstract The physical distance between presynaptic Ca 2+ channels and the sensors triggering release of neurotransmitter-containing vesicles regulates short-term plasticity (STP). While STP is highly diversified across synapse types, computational behavioral relevance this diversity remains unclear. In Drosophila brain, at nanoscale level, we can distinguish distinct coupling distances (m)unc13 family priming factors, Unc13A Unc13B. Importantly, defines components with characteristics. Here,...
Abstract At presynaptic active zones (AZs), scaffold proteins are critical for coordinating synaptic vesicle release and forming essential nanoarchitectures. However, regulatory principles steering AZ assembly, function, plasticity remain insufficiently understood. We here identify an additional Drosophila protein, “Blobby”, proper nano-organization. Blobby biochemically associates with the ELKS family protein Bruchpilot (BRP) integrates into newly AZs. Loss of results in fewer AZs forming,...
The cellular analysis of mushroom body (MB)-dependent memory forming processes is far advanced, whereas, the molecular and physiological understanding their synaptic basis lags behind. Recent Drosophila olfactory system showed that Unc13A, a member M(Unc13) release factor family, promotes phasic, high probability component, while Unc13B supports slower tonic reflecting different nanoscopic positioning within individual active zones. We here use STED super-resolution microscopy MB lobe...
Abstract At presynaptic active zones (AZs), scaffold proteins play a crucial role in coordinating synaptic vesicle (SV) release and forming intricate nanoarchitectures essential for function. Despite their suspected importance, factors governing the assembly of nanoscale AZ scaffolds have remained elusive. Here, we identify “Blobby” as novel regulator nanopatterning, localized within scaffold. Genetic loss extended Blobby protein led to aberrant accumulation (“blobs”) disrupted architecture...