A5089743031- Fungal and yeast genetics research
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- RNA and protein synthesis mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Cardiovascular Function and Risk Factors
- Plant Gene Expression Analysis
- Synthesis and biological activity
- Endoplasmic Reticulum Stress and Disease
- Genetics, Aging, and Longevity in Model Organisms
- Biofuel production and bioconversion
- Microbial Inactivation Methods
- Neuroscience and Neural Engineering
- Cell death mechanisms and regulation
- Bioinformatics and Genomic Networks
- GABA and Rice Research
- Postharvest Quality and Shelf Life Management
- Sirtuins and Resveratrol in Medicine
- 14-3-3 protein interactions
- Politics and Society in Latin America
- Chemical Reactions and Isotopes
Sapienza University of Rome
2007-2023
Istituto Pasteur
2005-2021
University of Southampton
2012
Centro di Riferimento Oncologico
2012
Charles Darwin University
2010
Fundación José Ortega y Gasset-Gregorio Marañón
2009
New arylthioindoles along with the corresponding ketone and methylene compounds were potent tubulin assembly inhibitors. As growth inhibitors of MCF-7 cells, sulfur derivatives superior or sometimes equivalent to ketones, while substantially less effective. Esters 24, 27−29, 36, 39, 41 showed ∼50% inhibition on human HeLa HCT116/chr3 cells at 0.5 μM, these inhibited HEK, M14, U937 IC50's in 78−220 nM range. While murine macrophage J744.1 cell was significantly affected (20% higher...
Voltage-dependent anion-selective channels (VDACs) are pore-forming proteins allowing the permeability of mitochondrial outer membrane. The VDAC3 isoform is least abundant and active in a complementation assay performed yeast strain devoid porin-1. We swapped N-terminal 20 amino acids with homologous sequences from other isoforms. substitution N-terminus VDAC1 caused chimaera to become more than VDAC1. VDAC2 improved activity, though lesser extent. carrying was able complement lack porin...
In this work we report that carnitines, in particular acetyl-l-carnitine (ALC), are able to prolong the chronological aging of yeast cells during stationary phase. Lifespan extension is significantly reduced yca1 mutants as well rho(0) strains, suggesting protective effects pass through Yca1 caspase and mitochondrial functions. ALC can also prevent apoptosis pro-apoptotic mutants, pointing importance functions regulating aging. We demonstrate attenuates fission aged cells, indicating a...
In recent years, epidemiological and biochemical studies have shown that eating apples is associated with reduction of occurrence cancer, degenerative, cardiovascular diseases. This association often attributed to the presence antioxidants such as ascorbic acid (vitamin C) polyphenols. The substances hinder free radicals are also able protect cells from aging. our laboratory we used yeast, a unicellular eukaryotic organism, determine in vivo efficacy entire their components, flesh, skin...
In a previous paper we reported the construction of S. cerevisiae strain lacking essential gene LSM4, which could survive by introduction truncated form orthologous from Kluyveromyces lactis. This showed apoptotic hallmarks and other phenotypes, including an increased sensitivity to caffeine acetic acid. The suppression latter phenotype overexpressing yeast genes allowed isolation PGK1, encoding glycolytic enzyme phosphoglycerate kinase. restored normal ageing, oxygen peroxide resistance...
During the past years, a number of studies have demonstrated positive effect apple on ageing and different diseases such as cancer, degenerative cardiovascular diseases. The unicellular yeast Saccharomyces cerevisiae represents simple eukaryotic model to study effects compounds lifespan. We previously that extracts anti-ageing in this organism because their antioxidant properties. In particular, is related presence fruit polyphenols, which give large contribution activity apples.We we used...
Abstract Background Saccharomyces cerevisiae BY4741 is an auxotrophic commonly used strain. In this work it has been as host for the expression and secretion of human interleukin-1 β (IL1 ), using cell wall protein Pir4 fusion partner. To achieve high density and, consequently, product yield, [PIR4-IL1 ] was cultured in aerated fed-batch reactor, a defined mineral medium supplemented with casamino acids ACA (auxotrophy-complementing amino acid) source. Also S. mutant Δ yca1 ], carrying...
We report the study of Kluyveromyces lactis cells expressing a truncated form KlLSM4, gene ortholog to LSM4 Saccharomyces cerevisiae which encodes an essential protein involved in both pre-mRNA splicing and mRNA decapping. had previously demonstrated that first 72 amino acids K. Lsm4p (KlLsm4Deltap) can restore cell growth S. not endogenous protein. However, showed remarkable loss viability stationary phase. Here we KlLsm4Deltap presented clear apoptotic markers such as chromatin...
We previously have reported that Saccharomyces cerevisiae mutants expressing Kllsm4Delta1, a truncated form of the KlLSM4 gene, as well in genes mRNA-decapping pathway, show phenotypic markers apoptosis, increased temperature sensitivity and reduced growth presence different drugs oxidative stressing agents, such acetic acid H(2)O(2). To isolate multicopy extra-genic suppressors these defects, we transformed Kllsm4Delta1 mutant with yeast DNA library selected series clones showing resistance...
Expression of the histone genes is tightly coupled to rates DNA synthesis in yeast and mRNAs are modulated both transcriptionally post-transcriptionally. Trf4 Trf5, poly(A) polymerases, that mediates polyadenylation consequent degradation) Rrp6, an exosome component, play a role regulation mRNA levels. In this paper we show degradation mutant Kllsm4Δ1, induced early S-phase maintained at high level all along entire cell cycle due delay exit from and/or entry into M-phase. The overexpression...
Despite recent advances in understanding the complexity of RNA processes, regulation metabolism oxidized cellular RNAs and mechanisms through which ribonucleotides affect mRNA translation, consequently cell viability, are not well characterized. We show here that level is markedly increased a yeast decapping Kllsm4Δ1 mutant, accumulates mRNAs, ages much faster wild type strain undergoes regulated-cell-death. also found cells mutation rate increases during chronological life span indicating...
The tumor suppressor p53 plays a central role in the regulation of cellular growth and apoptosis. In yeast Saccharomyces cerevisiae, overexpression human leads to inhibition apoptotic cell death on minimal medium. present work, we show that p53-expressing cells are more susceptible after an stimulus such as H2O2. analysis mutants involved apoptosis-like suggests observed is Yca1 independent mainly mediated through Nuc1p.
Saccharomyces cerevisiae mutants in the essential gene LSM4, involved messenger RNA decapping, and expressing a truncated form of LSM4 yeast Kluyveromyces lactis (Kllsm4Δ1), show premature aging accompanied by presence typical markers apoptosis high sensitivity to oxidative stressing agents. We isolated multicopy extragenic suppressors these defects, transforming Kllsm4Δ1 mutant with DNA library selecting clones showing resistance acetic acid. Here we present one clones, carrying fragment...
In this paper we report the growth and aging of yeast colonies derived from single cells isolated by micromanipulation seeded one on separated plates to avoid interference surrounding colonies. We named procedure clonal life span, it could represent a third way studying together with replicative span chronological span. study observed over time formation cell mass similar human "senile warts" (seborrheic keratoses), skin lesions that often appear after 30 years increase in number size years....
RESUMEN: En el contexto de un doble proceso transición, tanto política como económica, caracterizado por las fuertes convulsiones económicas y reformas institucionales, Vicente Palermo intenta analizar período gestión gubernamental argentino entre 1989 1994. Se centra en estudio la interacción los poderes ejecutivo legislativo, describiendo vínculos que, torno a agenda se establecieron diferentes poderes, evaluando impacto esa sobre propias instituciones del régimen político.ABSTRACT: In the...
LSM4 is an essential yeast gene encoding a component of different LSM complexes involved in the regulation mRNA splicing, stability and translation. In previous papers we reported that expression S. cerevisiae K. lactis Lsm4 lacking C-terminal Q/N-rich domain null strain (Sclsm4D1) restored cell viability. Nevertheless, this transformed observed some phenotypes which are typical markers regulated death, Reactive Oxygen Species oxidated RNA accumulation. paper report similar truncation...