Eliora Violain Buyamin
A5093085826- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Immune responses and vaccinations
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Cell Adhesion Molecules Research
Agency for Science, Technology and Research
2023-2025
Genome Institute of Singapore
2023-2025
Summary Lack of diversity and proportionate representation in genomics datasets databases contributes to inequity healthcare outcomes globally 1,2 . The relationships human with biological biomedical phenotypes are pervasive 3 , yet remain understudied, particularly a single-cell context. Here we present the Asian Immune Diversity Atlas (AIDA), multi-national RNA-sequencing (scRNA-seq) healthy reference atlas immune cells. AIDA comprises 1,265,624 circulating cells from 619 donors 6...
Several X-linked genes escape from X chromosome inactivation (XCI), while differences in across cell types and tissues are still poorly characterized. Here, we developed scLinaX for directly quantifying relative gene expression the inactivated with droplet-based single-cell RNA sequencing (scRNA-seq) data. The differentially expressed analyses large-scale blood scRNA-seq datasets consistently identified stronger lymphocytes than myeloid cells. An extension of to a 10x multiome dataset...
Alternative splicing contributes to complex traits, but whether this differs in trait-relevant cell types across diverse genetic ancestries is unclear. Here we describe cell-type-specific, sex-biased and ancestry-biased alternative ~1 M peripheral blood mononuclear cells from 474 healthy donors the Asian Immune Diversity Atlas. We identify widespread differential splicing, most of which cell-type-specific. 11,577 independent cis-splicing quantitative trait loci (sQTLs), 607 trans-sGenes 107...
Abstract One of the two X chromosomes females is silenced through chromosome inactivation (XCI) to compensate for difference in dosage between sexes. Among X-linked genes, several genes escape from XCI, which could contribute differential gene expression However, differences across cell types and tissues are still poorly characterized because no methods directly evaluate under a physiological condition at cell-cluster resolution with versatile technology. Here, we developed method, s ingle-...