A5089464615- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
- Vibrio bacteria research studies
- Advanced biosensing and bioanalysis techniques
- Seventeenth-Century Political and Philosophical Thought
- Marxism and Critical Theory
- Political Theology and Sovereignty
- Microbial Inactivation Methods
- Innovation and Socioeconomic Development
- Magnetic and Electromagnetic Effects
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Identification and Quantification in Food
Technical University of Munich
2024-2025
Philipps University of Marburg
2020-2024
Stadtwerke Straubing (Germany)
2024
Max Planck Institute for Terrestrial Microbiology
2021
Loewe Center for Synthetic Microbiology
2020-2021
The University of Western Australia
2020
Bridge University
2018
Berlin Heart (Germany)
1908
Moscow Institute of Thermal Technology
1908
Textron Systems (United Kingdom)
1908
Vibrio natriegens is known as the world's fastest growing organism with a doubling time of less than 10 min. This incredible growth speed empowers V. chassis for synthetic and molecular biology, potentially replacing E. coli in many applications. While first genetic parts have been built tested natriegens, comprehensive toolkit containing well-characterized standardized did not exist. To close this gap, we created Marburg Collection—a highly flexible Golden Gate cloning toolbox optimized...
Abstract The fast-growing bacterium Vibrio natriegens has recently gained increasing attention as a novel chassis organism for fundamental research and biotechnology. To fully harness the potential of this bacterium, highly efficient genome editing methods are indispensable to create strains tailored specific applications. V. is able take up free DNA incorporate it into its by homologous recombination. This natural transformation mediate uptake multiple fragments, thereby allowing...
Abstract Background Pyruvate is a precursor for various compounds in the chemical, drug, and food industries therefore an attractive target molecule microbial production processes. The fast-growing bacterium Vibrio natriegens excels with its specific substrate uptake rate as unconventional chassis industrial biotechnology. Here, we aim to exploit traits of V. pyruvate fermentations low biomass concentrations. Results We inactivated dehydrogenase complex Δ vnp12 , which harbors deletions...
Abstract Vibrio natriegens is known as the world’s fastest growing organism with a doubling time of less than 10 minutes. This incredible growth speed empowers V. chassis for synthetic and molecular biology, potentially replacing E. coli in many applications. While first genetic parts have been built tested , comprehensive toolkit containing well-characterized standardized did not exist. To close this gap, we created Marburg Collection – highly flexible Golden Gate Assembly-based cloning...
Electroporation protocol Weinstock paper: Matthew T Weinstock,Eric D Hesek,Christopher M Wilson,Daniel G Gibson Vibrio natriegens as a fast-growing host for molecular biologyNature Methods volume13,pages849–851(2016) To prepare before: recovery medium (BHI + v2 salts (204 mM NaCl, 4.2 KCl, 23.14mM MgCl2), and 680 sucrose) sterile filtration * i have box of aliquots in the freezer pre heat them before use -A vial competent cells is retrieved from storage at −80 °C allowed to thaw on ice....
Abstract The fast-growing bacterium Vibrio natriegens has recently gained increasing attention as a novel chassis organism for wide range of projects. To fully harness the potential this fascinating bacterium, convenient and highly efficient genome editing methods are indispensable to create strains, tailored specific applications. V. is able take up free DNA incorporate it into its by homologous recombination. This process, called natural transformation, was tamed editing. It displays high...