A5104336575- Receptor Mechanisms and Signaling
- Nuclear Receptors and Signaling
- Neurology and Historical Studies
- Nicotinic Acetylcholine Receptors Study
- Neurological disorders and treatments
- Neuroscience and Neuropharmacology Research
- Parkinson's Disease Mechanisms and Treatments
National Institute of Neurological Disorders and Stroke
2024
National Institutes of Health
2024
This protocol describes preparation of acute midbrain slices, setup electrophysiology rig, and solutions for collecting whole cell recordings.
It has proven challenging to faithfully recapitulate the key pathological, physiological, and behavioral features of Parkinson Disease (PD) in animals. Here we used adeno-associated virus (AAV) vectors achieve cell type-specific overexpression wild-type human α-synuclein (αsyn) a fluorophore (mCherry) midbrain dopamine neurons model PD mice. We found that AAVs drove selective expression both αsyn mCherry neurons. In conjunction with approximately 2-fold αsyn, several histopathological...
Axons of dopaminergic neurons express gamma-aminobutyric acid type-A receptors (GABA