Ninon Zala
A5107874898- Photoreceptor and optogenetics research
- Protein Degradation and Inhibitors
- Photosynthetic Processes and Mechanisms
- Multiple Myeloma Research and Treatments
- Advanced Fluorescence Microscopy Techniques
- Click Chemistry and Applications
- Advanced Electron Microscopy Techniques and Applications
- Peptidase Inhibition and Analysis
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Algal biology and biofuel production
- Insect and Pesticide Research
- Photochromic and Fluorescence Chemistry
- bioluminescence and chemiluminescence research
- Entomopathogenic Microorganisms in Pest Control
- Chemical Synthesis and Analysis
- Cellular Mechanics and Interactions
- Cancer Research and Treatments
- Biocrusts and Microbial Ecology
- Enzyme Structure and Function
- Microbial Community Ecology and Physiology
- Microtubule and mitosis dynamics
- Advanced biosensing and bioanalysis techniques
- Cell Image Analysis Techniques
Institut de Biologie Structurale
2020-2024
Université Grenoble Alpes
2017-2024
CEA Grenoble
2017-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2017-2024
Centre National de la Recherche Scientifique
2020-2024
Grenoble Institute of Neurosciences
2017
Inserm
2017
Institut de Biosciences et Biotechnologies
2017
Laboratoire Biologie à Grande Échelle
2017
Invasive fungal infections cause significant morbidity and mortality among immunocompromised individuals, posing an urgent need for new antifungal therapeutic strategies. Here we investigate a chromatin-interacting module, the bromodomain (BD) from BET family of proteins, as potential target in Candida albicans, major human pathogen. We show that protein Bdf1 is essential C. albicans mutations inactivating its two BDs result loss viability vitro decreased virulence mice. report...
Abstract Cyt1Aa is the one of four crystalline protoxins produced by mosquitocidal bacterium Bacillus thuringiensis israelensis ( Bti ) that has been shown to delay evolution insect resistance in field. Limiting our understanding efficacy and path improved toxicity spectrum ignorance how crystallizes vivo its mechanism toxicity. Here, we use serial femtosecond crystallography determine protoxin structure from sub-micron-sized crystals . Structures determined under various pH/redox conditions...
Abstract Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis jegathesan , respectively. The naturally crystallize within host; however, crystals too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. was determined de novo using single-wavelength anomalous dispersion method, which turn...
Combining fluorescence and phosphorescence kinetics, we characterize forward reverse intersystem crossing (FISC RISC, respectively) between the singlet triplet manifolds S ↔ T in photoswitchable (rsEGFP2) non-photoswitchable (EGFP) green fluorescent proteins upon continuous 488 nm laser excitation at cryogenic temperatures (CTs). Both behave very similarly, with T1 absorption spectra showing a visible peak 490 (10 mM–1 cm–1) vibrational progression near-infrared (720 to 905 nm). The dark...
RsEGFP2 is a reversibly photoswitchable fluorescent protein used in super-resolved optical microscopies, which can be toggled between On state and nonfluorescent Off state. Previous time-resolved ultraviolet-visible spectroscopic studies have shown that the Off-to-On photoactivation extends over femto- to millisecond time scale involves two picosecond lifetime excited states four ground intermediates, reflecting trans-to-cis isomerization, deprotonation, structural reorganizations. Femto-...
Abstract Reversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance novel applications. Here we establish a link between photoswitching contrast, one the key parameters that dictate achievable resolution in nanoscopy applications, chromophore conformation non‐fluorescent state rsEGFP2, widely employed label REversible Saturable OpticaL Fluorescence Transitions...
The orange carotenoid protein (OCP) is a photoactive involved in cyanobacterial photoprotection by quenching of the excess light-harvested energy. photoactivation mechanism remains elusive, part due to absence data pertaining timescales over which structural changes take place. It also unclear whether or not oligomerization dark-adapted and light-adapted OCP could play role regulation its energy-quenching activity. Here, we probed photoinduced combination static time-resolved X-ray...
Abstract Reversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance novel applications. Here we establish a link between photoswitching contrast, key parameter that largely dictates the achievable resolution in nanoscopy applications, chromophore conformation non-fluorescent state rsEGFP2, widely employed label REversible Saturable OpticaL Fluorescence Transitions...
Abstract OaPAC is a recently discovered b lue-light u sing flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses triphosphate and translates light signal into production of cyclic monophosphate. Here, we report crystal structures enzyme in absence its natural substrate determined room temperature serial crystallography data collected at both an X-ray free electron laser synchrotron compare them with cryo macromolecular...
OaPAC is a recently discovered blue-light-using flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses triphosphate and translates light signal into production of cyclic monophosphate. Here, we report crystal structures enzyme in absence its natural substrate determined room-temperature serial crystallography data collected at both an X-ray free-electron laser synchrotron, compare these with cryo-macromolecular obtained...
Abstract The Orange Carotenoid Protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection, by quenching of the excess light harvested energy. photoactivation mechanism remains elusive, part due to absence data pertaining timescales over which structural changes take place. It also unclear whether or not oligomerization dark-adapted and light-adapted OCP could play role regulation its energy activity. Here, we probed photo-induced combination static time-resolved X-ray...
The Cover Feature displays a comparison of chromophore conformations in the X-ray crystal structures reversibly photoswitchable fluorescent protein rsEGFP2 its on state (green) and V151L (purple) V151A (yellow) variants their off states. latter an increased photoswitching contrast, as indicated by displayed switching kinetics. design Virgile ADAM. More information can be found Research Article Dominique Bourgeois, Martin Weik co-workers.
Abstract Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis jegathesan , respectively. The naturally crystallize within host; however, crystals too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography X-ray free electron lasers to in vivo -grown nanocrystals of these toxins. was determined de novo using single-wavelength anomalous dispersion method, which turn...