A5008580436- Physiological and biochemical adaptations
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Fish Ecology and Management Studies
- Ocular Oncology and Treatments
- High Altitude and Hypoxia
- Photosynthetic Processes and Mechanisms
- Aquatic Ecosystems and Biodiversity
- Histone Deacetylase Inhibitors Research
- Sperm and Testicular Function
- Aquaculture disease management and microbiota
- Environmental Toxicology and Ecotoxicology
- Pancreatic function and diabetes
- Photoreceptor and optogenetics research
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Spectroscopy and Quantum Chemical Studies
- Melanoma and MAPK Pathways
- Birth, Development, and Health
- Diabetes and associated disorders
- Cancer-related Molecular Pathways
- Neuroinflammation and Neurodegeneration Mechanisms
Leiden University Medical Center
2017-2025
Leiden University
1994-2020
Institut de Biologia Evolutiva
2001
Huygens Institute for History and Culture of the Netherlands
1990-1991
The current understanding of mammalian kidney development is largely based on mouse models. Recent landmark studies revealed pervasive differences in renal embryogenesis between and human. scarcity detailed gene expression data humans therefore hampers a thorough human the possible developmental origin diseases. In this paper, we present single-cell transcriptomics study fetal kidney. We identified 22 cell types host marker genes. Comparison samples from different ages continuous changes...
Many fish species adapt to hypoxia by reducing their metabolic rate and increasing hemoglobin-oxygen (Hb-O(2)) affinity. Pilot studies with young broods of cichlids showed that the could survive severe in contrast adults. It was therefore hypothesized early exposure results improved oxygen transport. This hypothesis tested using split brood experiments. Broods Astatoreochromis alluaudi, Haplochromis ishmaeli, a tilapia hybrid (Oreochromis) were raised either under normoxia (NR; 80-90% air...
Human pluripotent stem cell–derived islet (SC-islet) transplantation is a promising β cell replacement therapy for patients with type 1 diabetes, offering potential unlimited supply. Yet, the heterogeneity of final product containing non–target types has relevant implications SC-islet function, transplant volume, and safety. Here, we present clinically compliant, full three-dimensional differentiation protocol that includes purification step endocrine cell–rich clusters, relying on principle...
// Renier Heijkants 1 , Karen Willekens 2, 3 Mark Schoonderwoerd 4 Amina Teunisse Maaike Nieveen Enrico Radaelli 5 Luuk Hawinkels Jean-Christophe Marine and Aart Jochemsen Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands 2 Laboratory for Cancer VIB Center Leuven, Belgium Oncology, KU Gastroenterology-Hepatology, Mouse Histopathology Core Facility, the Biology Disease, Correspondence to: Jochemsen, email: A.G.Jochemsen@lumc.nl Keywords:...
Abstract Background If European silver eels are prevented from reproductive migration, they remain in a prepubertal stage by dopaminergic inhibition of pituitary activity. Because this is likely requirement for an extended female growth stage, we tested if it sex-specific subjecting both sexes to stimulation GnRHa (Gonadotropin-Releasing Hormone agonist) – injection or 3-months swimming seawater. Results In contrast females, males showed two- three-fold higher LHβ (luteinising hormone β...
When European silver eels (Anguilla anguilla) venture into the Atlantic Ocean for their 6,000 km semelparous spawning run to Sargasso Sea, they are still in a prepubertal stage. Further sexual development appears be blocked by dopaminergic inhibition of hypothalamus and pituitary activity. Recently, we found that swimming several weeks freshwater stimulated incorporation fat droplets oocytes. So, it was hypothesized long term seawater would release further also stimulate production...
Abstract Uveal melanoma (UM) is the most frequent ocular cancer in adults, accounting for ~5% of total incidence. Although primary tumor well treatable, patients frequently develop metastases which no curative therapy exists. Highly activated protein kinase C (PKC) a common feature UM and has shown potential as therapeutic intervention patients. Unfortunately, PKC inhibition single treatment appears to have only limited clinical benefit. Combining with activation p53, rarely mutated UM, by...
Broods of the Lake Victoria cichlid Haplochromis ishmaeli raised under hypoxic and normoxic conditions, showed striking differences in isohemoglobin (isoHb) pattern not observed two other cichlids that do belong to species flock (Rutjes et al., 2007). We therefore hypothesized adaptive mechanism seen H. response hypoxia constitutes a trait inherited from ancestors lived environments. tested this hypothesis by designing split-brood experiments with three representative same flock:...
Hypoxic conditions is a common adverse environmental condition in an aquatic environment. To study the responses of fish to this phenomenon carp (Cyprinus carpio L.) were exposed graded hypoxia load and oxygen consumption was measured continuously. At 30%AS (air saturation) 20%AS, 10%AS, 5%AS 3%AS blood, liver white muscle samples collected. In haematological parameters, substrates (FFA, lactate glucose), as well stress hormone cortisol measured. High-energy phosphorylated compounds tissue....
Tilapia Oreochromis mossambicus were exposed to water at pH 4·0 for 37 days. The was acidified slowly over 6 h enabling the animals acclimate and preventing damage of gill epithelium. Additional stressors, e.g. aluminium ions handling stress, avoided. No mortality or decreased food consumption observed during exposure period. significant changes between control acid groups energy rich compounds related parameters, i.e. adenylate charge, pool total adenine nucleotides, IMP load white muscle...
Carp were cannulated in the dorsal aorta, and after 2 days of recovery they infused with 1) norepinephrine, 2) yohimbine (alpha(2)-antagonist) plus 3) clonidine (alpha(2)-agonist), 4) isoproterenol (nonselective beta-agonist). Norepinephrine lowered plasma free fatty acid (FFA) level raised glucose for several hours. combination alpha(2)-antagonist resulted retardation FFA decrease, indicating involvement alpha(2)-adrenoceptors. Infusion partial alpha(2)-agonist had a smaller effect. caused...
Background: β-cell replacement therapy by allogeneic pancreas or islet transplantation is a promising approach for patients with type 1 diabetes. However, there scarcity of organ donors. The generation insulin-producing pancreatic β-cells from human pluripotent stem cells (hPSC) in vitro would provide an unlimited cell source drug discovery and Here we aim to generate under GMP (Good Manufacturing Practice)-compliant conditions that can be used clinical application. Methods: We applied...