A5103624788- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- COVID-19 epidemiological studies
- Long-Term Effects of COVID-19
- Animal Virus Infections Studies
- COVID-19 and healthcare impacts
- Respiratory viral infections research
- Bacterial Infections and Vaccines
- Advanced biosensing and bioanalysis techniques
- Urinary Bladder and Prostate Research
- Disaster Response and Management
- Viral Infections and Outbreaks Research
- Pneumonia and Respiratory Infections
- Biosensors and Analytical Detection
- Viral gastroenteritis research and epidemiology
- Urological Disorders and Treatments
- Herpesvirus Infections and Treatments
- Respiratory Support and Mechanisms
- Sepsis Diagnosis and Treatment
- IgG4-Related and Inflammatory Diseases
- COVID-19 Impact on Reproduction
- Acute Kidney Injury Research
- CRISPR and Genetic Engineering
- Nosocomial Infections in ICU
Sheffield Teaching Hospitals NHS Foundation Trust
2013-2024
Northern General Hospital
2010-2024
St Mary's Hospital
2023
International Severe Acute Respiratory and Emerging Infection Consortium
2023
University of Sheffield
2009-2022
Centre for Inflammation Research
2022
University of Edinburgh
2022
The Queen's Medical Research Institute
2022
NIHR Leicester Biomedical Research Centre
2022
University of Leicester
2022
A SARS-CoV-2 variant carrying the Spike protein amino acid change D614G has become most prevalent form in global pandemic. Dynamic tracking of frequencies revealed a recurrent pattern G614 increase at multiple geographic levels: national, regional, and municipal. The shift occurred even local epidemics where original D614 was well established prior to introduction variant. consistency this highly statistically significant, suggesting that may have fitness advantage. We found grows higher...
Healthcare workers (HCW) are potentially at increased risk of infection with coronavirus disease (COVID-19) and may transmit severe acute respiratory syndrome 2 (SARS-CoV-2) to vulnerable patients. We present results from staff testing Sheffield Teaching Hospitals NHS Foundation Trust, United Kingdom. Between 16 29 March 2020, 1,533 symptomatic HCW were tested, whom 282 (18%) positive for SARS-CoV-2. Testing is a crucial strategy optimise staffing levels during this outbreak.
Background. Herd protection by meningococcal vaccines is conferred population-level reduction of nasopharyngeal colonization. Given the inverse epidemiological association between colonization commensal Neisseria lactamica and disease, we investigated whether controlled infection human volunteers with N. prevents meningitidis.
Hospital outbreaks of COVID19 result in considerable mortality and disruption to healthcare services yet little is known about transmission within this setting. We characterise hospital by combining viral genomic epidemiological data using Bayesian modelling amongst 2181 patients workers from a large UK NHS Trust. Transmission events were compared between Wave 1 (1st March 25th J'uly 2020) 2 (30th November 2020 24th January 2021). show that staff-to-staff transmissions reduced 31.6% 12.9%...
We have developed periscope, a tool for the detection and quantification of subgenomic RNA (sgRNA) in SARS-CoV-2 genomic sequence data. The translation genome most open reading frames (ORFs) occurs via intermediates termed "subgenomic RNAs." sgRNAs are produced through discontinuous transcription, which relies on homology between transcription regulatory sequences (TRS-B) upstream ORF start codons that TRS-L, is located 5' UTR. TRS-L immediately preceded by leader sequence. This therefore...
LamPORE is a novel diagnostic platform for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA combining loop-mediated isothermal amplification with Nanopore sequencing, which could potentially be used to analyze thousands samples per day on single instrument. We evaluated performance against reverse transcriptase PCR (RT-PCR) using extracted from spiked and stored nose throat swabs collected at two UK hospitals.
Background. Natural immunity to Neisseria meningitidis may result from nasopharyngeal carriage of closely related commensals, such as lactamica.
Abstract SARS-CoV-2 lineage B.1.1.7 viruses are more transmissible, may lead to greater clinical severity, and result in modest reductions antibody neutralization. subgenomic RNA (sgRNA) is produced by discontinuous transcription of the genome a crucial step life cycle. Applying our tool (periscope) ARTIC Network Oxford Nanopore genomic sequencing data from 4400 positive samples, we show that normalised sgRNA expression profiles significantly increased infections (n=879). This increase seen...
ABSTRACT Natural immunity to meningococcal disease in young children is associated epidemiologically with carriage of commensal Neisseria species, including lactamica . We have previously demonstrated that outer membrane vesicles (OMVs) from N. provide protection against lethal challenge a mouse model septicemia. evaluated the safety and immunogenicity an OMV vaccine phase I placebo-controlled, double-blinded clinical trial. Ninety-seven healthy adult male volunteers were randomized receive...
The importance of appropriate personal protective equipment (PPE) as a component healthcare worker (HCW) protection was highlighted during the Ebola virus disease (EVD) outbreak in West Africa. large number HCW deaths Africa part due to lack resources or prior training PPE usage. As legacy, High Consequence Infectious Disease (HCID) programme initiated by NHS England and Public Health (PHE) improve preparedness for other infections that not only endanger life patient, but also pose...
B.1.1.7 lineage SARS-CoV-2 is more transmissible, leads to greater clinical severity, and results in modest reductions antibody neutralization. Subgenomic RNA (sgRNA) produced by discontinuous transcription of the genome. Applying our tool (periscope) ARTIC Network Oxford Nanopore Technologies genomic sequencing data from 4400 positive samples, we show that normalised sgRNA significantly increased (alpha) infections (n = 879). This increase seen over previous dominant UK, B.1.177 943), which...
Background Respiratory syncytial virus (RSV) is an important cause of acute respiratory infection (ARI) in older adults. Vaccines that protect against severe RSV are now available. Aim We aimed to describe the incidence, presentation, severity and clinical outcomes RSV-associated ARI hospitalised adults using a new Hospital-based Sentinel Surveillance (HARISS) system England winter prior vaccine introduction. Methods Adults aged ≥65 years from seven hospitals admitted for ≥24 hours with...
Summary LamPORE is a novel diagnostic platform for the detection of SARS-CoV-2 RNA that combines loop-mediated isothermal amplification with nanopore sequencing, which could potentially be used to analyse thousands samples per day on single instrument. We evaluated performance against RT-PCR using extracted from spiked respiratory and stored nose throat swabs collected at two UK hospitals. The limit was 7-10 genome copies/µl RNA. This above achievable by but not associated significant...
Summary— Fifty‐two male patients were studied prospectively to assess the results of direct vision urethrotomy in treatment urethral strictures. The prognosis was found be significantly worse those who had received extensive previous treatment. A catheter is recommended for at least 3 days and 4 weeks have minimal or impaired detrusor function. use a urine flowmeter essential determining success stricture recurrence. Measurement recurrence‐free period important assessing progressive...
This study explores the relationship between mortality and late presentation for dialysis, focusing on role of catheter access hemodialysis (HD).We analyzed data from a cohort 286 patients commencing dialysis in 2000-2001. Survival factors associated with death were by univariate multivariate analysis. Dialysis was considered three groups: HD-AVF, HD-Catheter, peritoneal (PD). Late referral (LR) defined as first review nephrologist less than 90 days before dialysis.One-year low at 10.1%....
Summary— Impotence in males is a well recognised complication of diabetes mellitus, commonly held to be due an autonomic neuropathy. The aim this study was determine the incidence periphero‐conus neuropathy diabetic impotence. Nineteen seeking treatment for loss potency had bulbocavernosus reflex latency (BCRL) and sacral latencies (SRLs) performed. conduction studies indicated that impotence these cases associated with peripheral 21% patients BCRL SRLs are sensitive useful diagnostic tests.
Structured abstract Objectives To characterise within-hospital SARS-CoV-2 transmission across two waves of the COVID-19 pandemic. Design A retrospective Bayesian modelling study to reconstruct chains amongst 2181 patients and healthcare workers using combined viral genomic epidemiological data. Setting large UK NHS Trust with over 1400 beds employing approximately 17,000 staff. Participants 780 522 staff testing positive between 1st March 2020 25th July (Wave 1); 580 299 30th November 24th...
Summary— Twenty‐two patients with intermittent loin pain thought to be typical of primary pelvic hydronephrosis but normal appearances at standard excretion urography were subjected diuretic using Frusemide‐induced diuresis. If this was normal, acute performed when the patient had pain. Abnormal shown in only 15 patients. In 4, erect films revealed hydronephrosis. Dilatation occurred response a load 9 and abnormality time an attack 4. The during 2, urogram having been normal. 2 nephrographic...
No AccessJournal of Urology1 Jan 1983The Importance Urodynamic Investigation Following Spontaneous Rupture the Bladder A.D. Desmond, K.A. Woolfenden, and C.M. Evans DesmondA.D. Desmond , WoolfendenK.A. Woolfenden EvansC.M. View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)51962-XAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Abstract: We report a case spontaneous intraperitoneal rupture bladder....