A5050069758- Cancer Genomics and Diagnostics
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Oral microbiology and periodontitis research
- Colorectal Cancer Treatments and Studies
- Lung Cancer Treatments and Mutations
- Lymphoma Diagnosis and Treatment
- Streptococcal Infections and Treatments
- Enzyme Catalysis and Immobilization
- Genetic factors in colorectal cancer
- Urinary and Genital Oncology Studies
- Genomics and Phylogenetic Studies
- Peptidase Inhibition and Analysis
- Prostate Cancer Treatment and Research
- Cancer Immunotherapy and Biomarkers
- Fungal and yeast genetics research
- RNA modifications and cancer
- Bladder and Urothelial Cancer Treatments
- Lung Cancer Research Studies
- Epigenetics and DNA Methylation
- Bacterial biofilms and quorum sensing
- Oral and Maxillofacial Pathology
- BRCA gene mutations in cancer
- Vascular Malformations and Hemangiomas
- Thyroid Cancer Diagnosis and Treatment
Bundeswehrkrankenhaus
2013-2025
Armed Forces Hospital
2024
Universität Ulm
2007-2022
Institute of Microbiology and Biotechnology
2007
Molecular modifications of the androgen receptor (AR) can cause resistance to deprivation therapy (ADT) in prostate cancer patients. Since lack representative tumor samples hinders adjustments according emerging AR-modifications, we evaluated simultaneous detection two most common AR (AR-V7 splice variant and
// Anna-Maria Bubolz 1 , Stephanie E. Weissinger Albrecht Stenzinger 2 Annette Arndt 3 Konrad Steinestel 3,4 Silke Brüderlein Holger Cario 5 Anneli Lubatschofski Claudia Welke 6 Ioannis Anagnostopoulos 7 Thomas F. Barth Ambros J. Beer 8 Peter Möller Martin Gottstein Andreas Viardot 9* and Jochen K. Lennerz 1* Institute of Pathology, University Ulm, Germany Hospital Heidelberg, Pathology Molecular Bundeswehrkrankenhaus 4 Bundeswehr Radiobiology, Munich, Department Pediatric...
Testing for epidermal growth factor receptor (EGFR) mutations is an essential recommendation in guidelines metastatic non-squamous non-small-cell lung cancer, and considered mandatory European countries. However, practice, challenges are often faced when carrying out routine biomarker testing, including access to inadequate tissue samples long turnaround times (TATs).To evaluate the real-world EGFR testing practices of pathology laboratories, online survey was set up validated by Pulmonary...
ABSTRACT Corynebacterium glutamicum has recently been shown to grow on ethanol as a carbon and energy source possess high alcohol dehydrogenase (ADH) activity when growing this substrate low ADH plus glucose or alone. Here we identify the C. gene ( adhA ), analyze its transcriptional organization, investigate relevance of regulators acetate metabolism RamA RamB for expression. Sequence analysis predicts polypeptide 345 amino acids showing up 57% identity with zinc-dependent enzymes group I....
<i>Corynebacterium glutamicum</i> grows on a variety of carbohydrates and organic acids as single or combined sources carbon energy. Here we show the ability <i>C. to grow ethanol with growth rates up 0.24 h<sup>–1</sup> biomass yields 0.47 g dry weight (g ethanol)<sup>–1</sup>. Mutants deficient in phosphotransacetylase (PTA), isocitrate lyase (ICL) malate synthase (MS) were unable ethanol, indicating that acetate activation glyoxylate cycle are...
Up to 50% of penile squamous cell carcinomas (pSCC) develop in the context high-risk human papillomavirus (HR-HPV) infection. Most these tumours have been reported show basaloid differentiation and overexpression tumour suppressor protein p16(INK4a). Whether HPV-triggered carcinogenesis pSCC has an impact on aggressiveness, however, is still subject research.In tissue specimens from 58 patients with surgically treated between 1995 2012, we performed p16(INK4a) immunohistochemistry DNA...
Background: Malignant neoplasms of the salivary glands are rare, and therapeutic options limited. Results from recently published studies indicate a possible use for checkpoint inhibition in subset patients, but there no established criteria programme cell death ligand 1 (PD-L1) scoring gland carcinomas (SGCs). Methods: In this retrospective study, we present cohort 94 SGC patients with full clinical follow-up. We included 41 adenoid cystic (AdCC), 21 mucoepidermoid (MEC), 16 acinic (ACC),...
Dental plaque formation is a developmental process involving cooperation and competition within diverse microbial community, approximately 70 % of which composed an array streptococci during the early stages supragingival formation. In this study, 79 cell-free culture supernatants from variety oral were screened to identify extracellular compounds that inhibit biofilm by anaerobe Porphyromonas gingivalis strain 381. The majority streptococcal (61 isolates) resulted in lysis P. cells, some...
Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality worldwide. Advances in tissue-based biomarkers have significantly enhanced diagnostic and therapeutic approaches NSCLC, enabling precision medicine strategies. This review provides a comprehensive analysis molecular pathologist&rsquo;s practical approach to assessing NSCLC across various specimen types (liquid biopsy, broncho-alveolar lavage, transbronchial biopsy/ endobronchial ultrasound-guided biopsy...
Abstract In classic Hodgkin-lymphoma (cHL), only a few cases recur, and limited fraction of patients is primary-refractory to standard-polychemotherapy. Underlying genomic features unfavorable clinical courses remain sparsely characterized. Here, we investigated the characteristics primary-refractory/relapsed cHL in contrast with responders. Therefore, ultra-deep next-generation panel-sequencing was performed on total 59 FFPE-samples (20 responders, 26 relapsed (rHL: 11 initial-diagnosis, 15...
Pyruvate dehydrogenase complex-deficient strains of Corynebacterium glutamicum produce L-valine from glucose only after depletion the acetate required for growth. Here we show that inactivation DeoR-type transcriptional regulator SugR or replacement by ethanol already in course growth phase results efficient production.
The role of human papillomavirus (HPV) in bladder carcinogenesis remains controversial. Overexpression p16(INK4a), a surrogate marker for infection with oncogenic HPV other tumours, has been described urothelial carcinoma situ (UCIS). Our goal was therefore to evaluate whether overexpression p16(INK4a) is associated and identify mechanisms upregulation UCIS.In 60 tissue specimens from total 45 patients (UCIS controls), we performed immunohistochemistry followed by detection subclassification...
HU is a non-sequence-specific DNA-binding protein and one of the most abundant nucleoid-associated proteins in bacterial cell. Like Escherichia coli, genome Porphyromonas gingivalis predicted to encode both HUα (PG1258) HUβ (PG0121) subunit. We have previously reported that PG0121 encodes non-specific co-transcribed with K-antigen capsule synthesis operon. also deletion resulted downregulation operon expression produced P. strain phenotypically deficient surface polysaccharide production....
5067 Background: Molecular modifications of the androgen receptor (AR) can cause resistance to deprivation therapy (ADT) and chemotherapy in prostate cancer patients. When ensues, lack representative tumor samples hinders adjustments according emerging AR-modifications some patients may thus receive ineffective therapy. Methods: We devised a single-tube assay detect two most common (AR-V7 splice variants ARpoint mutations) circulating cells (CTC) using immunomagnetic CTC isolation followed...
ABSTRACT K-antigen capsule synthesis is an important virulence determinant of the oral anaerobe Porphyromonas gingivalis . We previously reported that locus required for this surface polysaccharide in strain W83 (TIGR identification PG0106 to PG0120) transcribed as a large (∼16.7-kb) polycistronic message. Through sequence analysis, we have now identified 77-bp inverted repeat located upstream (206 bp) start codon capable forming hairpin structure. Further analysis just and downstream genes...
Abelson interactor 1 (Abi1) is an important regulator of actin dynamics during cytoskeletal reorganization. In this study, our aim was to investigate the expression Abi1 in colonic mucosa with and without inflammation, polyps, colorectal carcinomas (CRC) metastases as well CRC cell lines respect BRAF/KRAS mutation status find out whether introduction KRAS or stimulation TNFalpha enhances protein cells.We immunohistochemically analyzed 126 tissue specimens from 95 patients 5 carcinoma...
Abstract Childhood radiation exposure has been associated with increased papillary thyroid carcinoma (PTC) risk. The role of anaplastic lymphoma kinase ( ALK ) gene rearrangements in radiation‐related PTC remains unclear, but STRN‐ALK fusions have recently detected PTCs from exposed persons after Chernobyl using targeted next‐generation sequencing and RNA‐seq. We investigated RET as well known driver point mutations tumours 77 radiation‐exposed patients (mean age at surgery 22.4 years) 19...