A5032330087- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Thyroid Cancer Diagnosis and Treatment
- Colorectal Cancer Treatments and Studies
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Lymphoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Research Studies
- Sphingolipid Metabolism and Signaling
- Hedgehog Signaling Pathway Studies
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- Molecular Biology Techniques and Applications
- Salivary Gland Tumors Diagnosis and Treatment
- PI3K/AKT/mTOR signaling in cancer
- Radiomics and Machine Learning in Medical Imaging
- Ubiquitin and proteasome pathways
- Cytokine Signaling Pathways and Interactions
- Neuroendocrine Tumor Research Advances
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Ovarian cancer diagnosis and treatment
University of Naples Federico II
2016-2025
Federico II University Hospital
2016-2025
Beth Israel Deaconess Medical Center
2023-2024
The University of Texas MD Anderson Cancer Center
2019-2024
Università Cattolica del Sacro Cuore
2017-2024
Massachusetts General Hospital
2016-2024
Faculty of Media
2023
European School of Molecular Medicine
2007-2023
University of Modena and Reggio Emilia
2023
University of Oradea
2023
MicroRNAs (miRNAs) are a class of small non-coding RNAs involved in wide range basic processes such as cell proliferation, development, apoptosis and stress response. It has recently been found that they also abnormally expressed many types human cancer. We analyzed the genome-wide miRNA expression profile thyroid papillary carcinomas (PTCs) using microarray (miRNACHIP microarray) containing hundreds precursor mature oligonucleotide probes. Using this approach, we an aberrant clearly...
Background : Medullary and papillary thyroid carcinomas are often associated with oncogenic activation of the RET tyrosine kinase. We evaluated whether biaryl urea BAY 43-9006, which is known to inhibit several other kinases, blocks kinase function activity. Methods examined 43-9006 activity against in vitro cellular signaling RET-transfected NIH3T3 fibroblasts by using immunocomplex assays immunoblotting phospho-specific antibodies. The effects on proliferation human TPC1 TT carcinoma...
Point mutations in BRAF are genetic hallmarks of papillary thyroid carcinoma (PTC). In this retrospective study, we examined aspirates and corresponding paraffin-embedded surgical samples for the presence mutations. Altogether, 96 cases, including 69 PTCs, 19 follicular adenomas, eight nontoxic nodular goiters BRAF; 60 these were also RET/PTC rearrangements. The results correlated with cytological diagnosis final histopathology. mutation (V599E) was detected 38% that PTC on histopathology;...
Alterations in chromosome number (aneuploidy) are common human neoplasias. Loss of mitotic regulation is believed to induce aneuploidy cancer cells and act as a driving force during the malignant progression. The serine/theronine protein kinases aurora family genes play critical role key cell cycle processes. Aurora B mediates segregation by ensuring orientation sister chromatids overexpression diploid NHDF (normal fibroblast) induces multinuclearity. We analyzed expression thyroid...
The role of statins in the prevention contrast-induced acute kidney injury (CIAKI) is controversial.First, we investigated vivo effects atorvastatin on CIAKI. Patients with chronic disease enrolled Novel Approaches for Preventing or Limiting Events (NAPLES) II trial were randomly assigned to (1) group (80 mg within 24 hours before contrast media [CM] exposure; n=202) (2) control (n=208). All patients received a high dose N-acetylcysteine and sodium bicarbonate solution. Second, vitro...
Abstract Purpose: Oncogenic conversion of BRAF occurs in ∼44% papillary thyroid carcinomas and 24% anaplastic carcinomas. In carcinomas, this mutation is associated with an unfavorable clinicopathologic outcome. Our aim was to exploit as a potential therapeutic target for carcinoma. Experimental Design: We used RNA interference evaluate the effect knockdown human carcinoma cell lines FRO ARO carrying V600E (V600EBRAF) mutation. also exploited BAY 43-9006...
Summary Background Fine‐needle aspiration biopsy (FNAB) is the primary means to distinguish benign from malignant nodules and select patients for surgery. However, adjunctive diagnostic tests are needed because in 20–40% of cases FNAB result uncertain. Objective We investigated whether a search oncogenes RET/PTC , TRK BRAF V600E thyroid aspirates could refine an uncertain diagnosis. Patients methods A total 132 aspirates, including colloid nodules, inadequate samplings, indeterminate...
The CBX7 gene encodes a polycomb group protein that is known to be downregulated in many types of human cancers, although the role this carcinogenesis remains unclear. To shed light on issue, we generated mice null for Cbx7. Mouse embryonic fibroblasts derived from these had higher growth rate and reduced susceptibility senescence compared with their WT counterparts. This was associated upregulated expression multiple cell cycle components, including cyclin E, which play key lung humans....
Contrast-induced nephropathy accounts for >10% of all causes hospital-acquired renal failure, a prolonged in-hospital stay and represents powerful predictor poor early late outcome. Mechanisms contrast-induced are not completely understood. In vitro data suggests that contrast media (CM) induces direct toxic effect on tubular cells through the activation intrinsic apoptotic pathway. It is unclear whether this has role in clinical setting. work, we evaluated effects CM both vivo vitro. By...
When tumour tissue is unavailable, cell-free DNA (cfDNA)can serve as a surrogate for genetic analyses. Because mutated alleles in cfDNA are usually below 1%, next-generation sequencing (NGS)must be narrowed to target only clinically relevant genes. In this proof-of-concept study, we developed panel use ultra-deep identify such mutations cfDNA.Our ('SiRe') covers 568 six genes (EGFR, KRAS, NRAS, BRAF, cKIT and PDGFRα)involved non-small-cell lung cancer (NSCLC), gastrointestinal stromal...
Resistance to tyrosine kinase inhibitors (TKI) of EGF receptor (EGFR) is often related activation other signaling pathways and evolution through a mesenchymal phenotype.Because the Hedgehog (Hh) pathway has emerged as an important mediator epithelial-to-mesenchymal transition (EMT), we studied Hh in models EGFR-TKIs intrinsic or acquired resistance from both EGFR-mutated wild-type (WT) non-small cell lung cancer (NSCLC) lines.Activation was found EGFR-WT NSCLC line resistant EGFR-TKIs. In...
Many advanced cases of cancer show central nervous system, pleural, or peritoneal involvement. In this study, we prospectively analyzed if cerebrospinal fluid (CSF), pleural effusion (PE), and/or ascites (ASC) can be used to detect driver mutations and guide treatment decisions. We collected 42 CSF, PE, ASC samples from non‐small‐cell lung melanoma patients. Cell‐free DNA (cfDNA) was purified quantified by PNA‐Q‐PCR next‐generation sequencing. All were evaluable; clinically relevant detected...