To find out the effect of prill fat feeding on milk production and hormonal changes in crossbred cows.Crossbred cows mid lactation (150 days) were selected from institutes' herd.The observations dry matter intake (DMI), body weight, condition score (BCS)were recorded composition, plasma metabolites glucose Nonesterified fatty acids (NEFA) measured control (CON) experimental group (PFG) cows.During period, PFG fed with bypass @ 75 g/d for a period 90 days.: The DMI weight was non-significant...

The present study investigated the effects of different dietary forage to concentrate ratios on animal performance, and enteric manure greenhouse gas emissions in growing calves. Fifteen male Murrah calves (153.5 ± 18.17 kg; 6 12 months) were randomly assigned 3 treatments fed corn fodder, wheat straw proportions: 20:60:20 (C20); 20:40:40 (C40) 10:30:60 (C60), for a period 120 days. Increasing proportion had no significant (P > 0.05) effect dry matter intake (DMI) but increased crude protein...

The objective of this study was to examine the effects FSH and LH on oestradiol-17β progesterone production by buffalo granulosa cells cultured under serum-free conditions. Granulosa (3 × 10(5) ) from small (≤ 5 mm diameter) follicles were for up 4 days in 48-well plates coated with 3.3 μg/cm(2) fibronectin Dulbecco's modified Eagle's medium (DMEM) : nutrient mixture F-12 Ham (1 1 ratio) supplemented 10(-7) m androstenedione, μg/ml human apo-transferrin 0.1% bovine serum albumin, presence or...

This experiment was conducted to study the effects of addition malic acid (MA) and tree leaves in straw based total mixed ration on vitro rumen fermentation parameters. Four experimental substrates were prepared: Control (C-wheat concentrate 1: 1 ratio), T1 (45% wheat + 45% 10% Leucaena leucocephala leaves), T2 (T1 diet 1% MA), T3 2% MA). Inclusion increased (P<0.05) true dry (IVTMD) organic matter (IVOMD) digestibility, however, no effect observed other Addition MA conjunction with gas...

Present study was designed to assess the seasonal influence of seminal plasma constituents on sperm attributes in crossbred cattle bulls. Semen ejaculates were collected from 10 Karan Fries bulls at fortnightly interval during hot-humid and winter months. The composition (hormones enzymes) evaluated using standard kits. ALT, AST ALP activities higher Seasonal variations not observed testosterone prostaglandin E2, whereas estradiol showed a significantly level season. Moreover, no correlation...

The host responses to disease agents differ between age and sex as is evidenced from epidemiological studies which have been ascribed the effects of steroid hormones. present study was carried out in 20 Murrah buffaloes either at pre- post-pubertal having discrete background explore variation, if any, physiological immune upon intravenous endotoxin challenge (E. coli 055:B5 @ 0.6µg/kg body weight). rectal temperature, heart rate respiration differed significantly base values only 1 h post...

The aim of this study was to investigate the variations in plasma triiodothyronine (T3) and thyroxine (T4) with advancement age determine their circadian patterns prepubertal pubertal Murrah buffaloes. T3 T4 were observed from day 1 24 months age. Significant higher levels after birth a gradual decrease concentrations occurred until 15 days mean ranged between 1.26–3.79 60.7–166 ng/ml, respectively, during 1–30 During 1–24 age, did not differ (p > 0.05) whereas significant < 0.0001) changes...

Twelve healthy lactating Murrah buffaloes of similar parity (3rd) between 90 and 120 days lactation, selected from the herd National Dairy Research Institute (Karnal, India) maintained at managemental practices as followed they were included in this experiment. The animals divided into two groups based on their production level previous lactation. average milk group 1 II was 9.3 6 lit/day, respectively. Blood collected these three occasions 10 apart. lymphocytes separated cultured RPMI 1640...

This study was undertaken in buffalo neonates born to vitamin E (dl-alpha-tocopherol acetate)-supplemented and non-supplemented Murrah buffaloes. Calves from E-supplemented buffaloes (n = 10; -supplemented calves [VeC]) control [CC]) constituted the treatment groups respectively. Two colostrum samples were taken at first post-partum milking again after 12 h dams for IgG estimation. Sampling of blood performed on days 0 (before feeding), 1, 3, 7, 14, 21, 28, 42, 56, 70, 84, 98, 112 126...

This study investigated the age related variations in luteinizing hormone (LH), androstenedione, testosterone, and total estrogens response to exogenous gonadotropin-releasing (GnRH) Holstein-Friesian (HF) × Tharparkar bull calves. Fifteen calves were selected and, based on their age, divided into Group I (14-16 months, n = 5), II (9-12 III (6-8 5). All administered with 10 µg of GnRH intramuscularly. Blood samples collected at an interval 30 min commencing 1 h prior treatment until 4...

The present study was undertaken to observe the effect of supplementing Aegle marmelos leaves on in vitro rumen fermentation and methane production different diets containing varied proportion roughage (R) concentrate (C). (Bael) supplemented at 4 levels (0, 5, 10 15%) three having (C) (wheat straw mixture) ratio 70:30, 50:50 30:70. Inclusion showed significant rise total gas (mL/g DM) among diets. Similarly, rate 15% improved (P&gt;0.05) IVTDMD IVTOMD (%) experimental Among VFA’s acetate...

Prostasomes are extracellular vesicles that fuse with sperms thereby improving its functional parameters. Present study aimed to isolate and characterise prostasomes from semen of KF bulls, investigate effects on parameters bull spermatozoa. Isolated were characterized respect the binding FITC-conjugated CD 26 antibodies, as well protein, cholesterol phospholipids content. Subsequently, supplementation (1mg/ml) investigated ROS production, Ca2+ signalling, mitochondrial membrane potential...

The present study aimed to evaluate the effects of dietary forage:concentrate ratios on growth performance and enteric faecal greenhouse-gas emissions from growing buffalo calves. Fifteen Murrah male calves (bodyweight = 233.35 ± 30.92 kg; 8–12 months age) were randomly assigned three groups that fed a mixture berseem fodder, wheat straw concentrate at 20:60:20 (C20), 20:40:40 (C40) 20:20:60 (C60) respectively, for 120 days. Enteric methane (CH4) production was estimated by sulfur...

Background: Cryopreservation is an invaluable technique yet it also known to be detrimental sperm function and fertility due cryo-injury concomitant generation of reactive oxidants. During laboratory manipulation for the cryopreservation freeze-thaw process, spermatozoa undergo osmotic stress, ionic imbalance, metabolic decoupling, membrane phase transition, destabilization cytoskeleton antioxidant depletion which communally hampers semen quality.Methods: With aim determining implications...

The postpartum anestrus and silent estrus are the challenging cause for low reproductive efficiency in buffaloes especially tropical climate conditions. This study was aimed at assessing effect of dietary supplementation vitamin E on resumption cyclic ovarian activity periparturient Murrah through plasma progesterone (P4) hormone profiles. (n=12) were selected during their late gestation based expected date calving that fall between November February, from ICAR-National Dairy Research...

Warts, caused by the human papillomavirus (HPV), are a common dermatologic condition that often leads to treatment-seeking due cosmetic concerns. Immunotherapy, particularly with intralesional vaccines, such as MMR, BCG, Candida, Trichophyton, vitamin D, interferon alpha-2b and Mw, is effective for recalcitrant warts enhancing cell-mediated immunity [1]. Adverse events from these vaccines rare. We report case of acute epididymo-orchitis following MMR vaccine plantar warts. A 19-year-old male...