A5015400112- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant and Biological Electrophysiology Studies
- Plant responses to water stress
- Single-cell and spatial transcriptomics
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Plant nutrient uptake and metabolism
- Advanced Chemical Sensor Technologies
- Greenhouse Technology and Climate Control
- Identification and Quantification in Food
- Gene Regulatory Network Analysis
- Spectroscopy and Chemometric Analyses
- Leaf Properties and Growth Measurement
Salk Institute for Biological Studies
2022-2025
Jimei University
2025
Howard Hughes Medical Institute
2023-2024
Hebrew University of Jerusalem
2024
La Jolla Alcohol Research
2023
Delineating the gene-regulatory programs underlying complex cell types is fundamental for understanding brain function in health and disease. Here, we comprehensively examined human epigenomes by probing DNA methylation chromatin conformation at single-cell resolution 517 thousand cells (399 neurons 118 non-neurons) from 46 regions of three adult male brains. We identified 188 characterized their molecular signatures. Integrative analyses revealed concordant changes methylation,...
SUMMARY Extensive studies of the reference plant Arabidopsis have enabled a deep understanding tissues throughout development, yet census cell types and states development is lacking. Here, we present single-nucleus transcriptome atlas seed-to-seed employing over 800,000 nuclei, encompassing diverse set across ten developmental stages, with spatial transcriptomic validation dynamic seed silique. Cross-organ analyses revealed transcriptional conservation heterogeneity within individual...
Abstract Leaf development is dynamic, adapting to environmental stress optimize resource use. For example, in response drought, Arabidopsis restricts leaf growth enhance water use efficiency. While this plastic well described at the physiological level, underlying transcriptional changes that facilitate adjustments remain poorly described. By constructing a ∼1 million single-nuclei transcriptome atlas, we demonstrate drought limits by advancing responses related maturation and aging....
Soil-free assays that induce water stress are routinely used to investigate drought responses in the plant Arabidopsis thaliana . Due their ease of use, research community often relies on polyethylene glycol (PEG), mannitol, and salt (NaCl) treatments reduce potential agar media, thus conditions laboratory. However, while these types can create phenotypes resemble those deficit experienced by soil-grown plants, it remains unclear how compare at transcriptional level. Here, we demonstrate...
Soil-free assays that induce water stress are routinely used to investigate drought responses in the plant Arabidopsis thaliana . Due their ease of use, research community often relies on polyethylene glycol (PEG), mannitol, and salt (NaCl) treatments reduce potential agar media, thus conditions laboratory. However, while these types can create phenotypes resemble those deficit experienced by soil-grown plants, it remains unclear how compare at transcriptional level. Here, we demonstrate...
Delineating the gene regulatory programs underlying complex cell types is fundamental for understanding brain functions in health and disease. Here, we comprehensively examine human epigenomes by probing DNA methylation chromatin conformation at single-cell resolution over 500,000 cells from 46 regions. We identified 188 characterized their molecular signatures. Integrative analyses revealed concordant changes methylation, accessibility, organization, expression across types, cortical areas,...
Soil-free assays that induce water stress are routinely used to investigate drought responses in the plant Arabidopsis thaliana . Due their ease of use, research community often relies on polyethylene glycol (PEG), mannitol and salt (NaCl) treatments reduce potential agar media, thus conditions laboratory. However, while these types can create phenotypes resemble those deficit experienced by soil-grown plants, it remains unclear how compare at transcriptional level. Here, we demonstrate...
Simple, soil-free assays that can mimic drought conditions are incredibly useful for investigating plant stress responses. Due to their ease of use, the research community often relies on polyethylene glycol (PEG), mannitol and salt treatments simulate in laboratory. However, while these types osmotic create phenotypes resemble those drought, it remains unclear how they compare at molecular level. Here, using transcriptomics, we demonstrate unable replicate signaling responses Arabidopsis...
Abstract Soil-free assays that induce water stress are routinely used to investigate drought responses in the plant Arabidopsis thaliana . Due their ease of use, research community often relies on polyethylene glycol (PEG), mannitol and salt (NaCl) treatments reduce potential agar media, thus conditions laboratory. However, while these types can create phenotypes resemble those deficit experienced by soil-grown plants, it remains unclear how compare at transcriptional level. Here, we...