A5101790707- Glycosylation and Glycoproteins Research
- Pneumonia and Respiratory Infections
- Enzyme Structure and Function
- Vibrio bacteria research studies
- Monoclonal and Polyclonal Antibodies Research
- Photosynthetic Processes and Mechanisms
- Bacterial Infections and Vaccines
- Carbohydrate Chemistry and Synthesis
- Machine Learning in Bioinformatics
- Influenza Virus Research Studies
- Veterinary medicine and infectious diseases
- Respiratory viral infections research
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Photoreceptor and optogenetics research
- Vector-Borne Animal Diseases
- HIV Research and Treatment
- Streptococcal Infections and Treatments
- Distributed and Parallel Computing Systems
- Protein purification and stability
- Spectroscopy and Quantum Chemical Studies
- Bacterial Genetics and Biotechnology
- Energy and Environment Impacts
- American Environmental and Regional History
- Hepatitis C virus research
University of St Andrews
2008-2021
University of Bristol
2005-2011
Whiston Hospital
1992
Pfizer (United States)
1989
Clostridium perfringens is a Gram-positive bacterium responsible for bacteremia, gas gangrene, and occasionally food poisoning. Its genome encodes three sialidases, nanH, nanI, nanJ, that are involved in the removal of sialic acids from variety glycoconjugates play role bacterial nutrition pathogenesis. Recent studies on trypanosomal (trans-) sialidases have suggested catalysis all may proceed via covalent intermediate similar to other retaining glycosidases. Here we provide further evidence...
ABSTRACT The E2 envelope glycoprotein of hepatitis C virus (HCV) binds to the host entry factor CD81 and is principal target for neutralizing antibodies (NAbs). Most NAbs recognize hypervariable region 1 on E2, which undergoes frequent mutation, thereby allowing evade neutralization. Consequently, there great interest in that conserved epitopes. One such NAb AP33, a mouse monoclonal antibody recognizes conserved, linear epitope potently neutralizes broad range HCV genotypes. In this study,...
IPS-1/MAVS/VISA/Cardif is an adaptor protein that plays a crucial role in the induction of interferons response to viral infection. In initial stage intracellular antiviral two RNA helicases, retinoic acid inducible gene-I (RIG-I) and melanoma differentiation-association gene 5 (MDA5), are independently able bind cytoplasm. The 62 kDa contains N-terminal caspase activation recruitment (CARD) domain associates with CARD regions RIG-I MDA5, ultimately leading type I interferons. As first step...
Streptococcus pneumoniae Neuraminidase A (NanA) is a multi-domain protein anchored to the bacterial surface. Upstream of catalytic domain NanA that conforms sialic acid-recognising CBM40 family CAZY (carbohydrate-active enzymes) database. This has been identified play critical role in allowing bacterium promote adhesion and invasion human brain microvascular endothelial cells, hence may key promoting meningitis. In addition, also reported activate host chemokines neutrophil recruitment...
The role of a water molecule (water A) located between the primary electron donor (P) and first acceptor bacteriochlorophyll (B(A)) in purple bacterial reaction center was investigated by mutation glycine M203 to leucine (GM203L). x-ray crystal structure GM203L shows that new residue packs such way A is sterically excluded from complex, but protein-cofactor system around site largely undisturbed. results absorbance resonance Raman spectroscopy were consistent with either removal hydrogen...
The kinetics of recombination the P(+)H(A)(-) radical pair were compared in wild-type reaction centers from Rhodobacter sphaeroides and seven mutants which free energy gap, ΔG, between charge separated states P(+)B(A)(-) was either increased or decreased. Five mutant RCs had been described previously, X-ray crystal structures two newly constructed complexes determined by crystallography. accelerated all with a smaller ΔG than wild-type, slowed having larger ΔG. difference state PH(A) ground...
Rift Valley fever phlebovirus (RVFV) is a clinically and economically important pathogen increasingly likely to cause widespread epidemics. RVFV virulence depends on the interferon antagonist non-structural protein (NSs), which remains poorly characterized. We identified stable core domain of NSs (residues 83–248), solved its crystal structure, novel all-helical fold organized into highly ordered fibrils. A hallmark pathology filament formation in infected cell nuclei. Recombinant virus...
// Tao Lu 2, * , Yanmei Zou 1, Guogang Xu 3 Jane A. Potter 4 Garry L. Taylor Qiuhong Duan 2 Qin Yang 5 Huihua Xiong 1 Hong Qiu Dawei Ye Peng Zhang Shiying Yu Xianglin Yuan Feng Zhu Yihua Wang 6 Hua Department of Oncology, Tongji Hospital, Huazhong University Science and Technology, Wuhan, 430030, China Biochemistry molecular biology, School Basic Medicine, Nanlou Respiratory Department, Chinese PLA General Beijing, 10083, BioMedical Research Complex, St Andrews, KY16 9ST, UK Pathology,...
HCV vaccine development is stymied by the high genetic diversity of virus and variability envelope glycoproteins. One strategy to overcome this identify conserved, functionally important regions-such as epitopes broadly neutralizing antibodies (bNAbs)-and use these a basis for structure-based design. Here, we report an anti-idiotype approach that has generated antibody mimics highly conserved epitope on E2. Crucially, mutagenesis screen was used antibody, designated B2.1 A, whose binding...
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner-Doudoroff pathway to metabolize both glucose galactose. It has been proposed that a part-phosphorylative occurs in parallel S. solfataricus, which the 2-keto-3-deoxygluconate kinase (KDGK) is for galactose metabolism. Recombinant KDGK protein was expressed Escherichia coli, purified crystallized 0.1 M sodium acetate pH 4.1 1.4 NaCl. crystal...
The rapid global spread of severe acute respiratory coronavirus 2 (SARS-CoV-2) has resulted in an urgent effort to find efficacious therapeutics. Broad-spectrum therapies which could be used for other pathogens confer advantages, as do those based on targeting host cells that are not prone the development resistance by pathogen. We tested intranasally delivered carbohydrate-binding module (CBM) therapy, termed Neumifil, is a CBM previously been shown offer protection against influenza virus...
The emergence of severe acute respiratory syndrome coronavirus (SARS-CoV-2) and its expansion to a worldwide pandemic resulted in efforts assess develop interventions reduce the disease burden. Despite introduction vaccine programmes against SARS-CoV-2, global incidence levels early 2022 remained high, demonstrating need for development physiologically relevant models, which are essential identification alternative antiviral strategies. hamster model SARS-CoV-2 infection has been widely...
X-ray crystallography has been used to investigate the extent of structural changes in mutants purple bacterial reaction center that assemble without a particular ubiquinone or bacteriopheophytin cofactor. In case bacteriopheophytin-exclusion mutant, which Ala M149 was replaced by Trp (AM149W), quality protein crystals improved over seen previous work minimizing illumination, time, and temperature during purification protocol carrying out crystal growth at 4 °C after overnight incubation 18...
The severe diarrhoeal disease cholera is caused by the gram-negative bacterium Vibrio cholerae and continues to be a major cause of morbidity mortality. Like many species V. inhabits an aquatic ecosystem, most isolates do not possess ability cholera. Of more than 200 known serotypes, only O1 O139 serogroups are highly pathogenic acknowledged epidemic disease.1 serogroup can divided into two groups: classical El Tor, with first pandemic, beginning in Asia 1817, subsequent five pandemics...