A5100363986- Parasitic Infections and Diagnostics
- Toxoplasma gondii Research Studies
- Coccidia and coccidiosis research
- Antibiotic Resistance in Bacteria
- Antimicrobial Peptides and Activities
- Amoebic Infections and Treatments
- Advanced battery technologies research
- Chemical synthesis and alkaloids
- Chemokine receptors and signaling
- Vibrio bacteria research studies
- Neuroscience and Neuropharmacology Research
- Immunotherapy and Immune Responses
- Antimicrobial Resistance in Staphylococcus
- Helminth infection and control
- Bacterial biofilms and quorum sensing
- Flavonoids in Medical Research
- Pharmacogenetics and Drug Metabolism
- Hormonal and reproductive studies
- Electrochemical sensors and biosensors
- Trypanosoma species research and implications
- Antibiotics Pharmacokinetics and Efficacy
- Traditional Chinese Medicine Analysis
- Neurogenesis and neuroplasticity mechanisms
- Pharmaceutical and Antibiotic Environmental Impacts
- Plant-based Medicinal Research
Huazhong Agricultural University
2023-2025
Jilin University
2021-2024
Southern University of Science and Technology
2024
Shanghai Zhangjiang Laboratory
2024
Guangzhou Institutes of Biomedicine and Health
2023
University of Hong Kong
2023
First Affiliated Hospital Zhejiang University
2023
Center of Hubei Cooperative Innovation for Emissions Trading System
2023
Quzhou City People's Hospital
2021
Cryptosporidium parvum is a globally distributed apicomplexan parasite infecting humans and/or animals. Like other apicomplexans, it possesses specialized secretory organelles in the zoites, which micronemes discharge molecules to facilitate movement and invasion of zoites. Although past recent studies have identified several proteins cryptosporidial micronemes, our understanding composition, pathways, domain-ligand interactions micronemal remains limited. This study identifies new protein,...
Exploring novel antimicrobial drugs and strategies has become essential to the fight MRSA-associated infections. Herein, we found that membrane-disrupted repurposed antibiotic salifungin had excellent bactericidal activity against MRSA, with limited development of drug resistance. Furthermore, adding effectively decreased minimum inhibitory concentrations clinical antibiotics Staphylococcus aureus. Evaluations mechanism demonstrated disrupted level H+ K+ ions using hydrophilic lipophilic...
Apicomplexan parasites possess several unique secretory organelles, including rhoptries, micronemes, and dense granules, which play critical roles in the invasion of host cells. The molecular content these organelles their biological have been well-studied Toxoplasma Plasmodium , but are underappreciated Cryptosporidium contains many medical veterinary importance. Only four proteins previously identified or proposed to be located one which, GP900, was confirmed using immunogold electron...
Electro-active microorganisms (EAMs) profoundly affect elemental geochemical cycling and environmental remediation processes. While the roles of microbial-secreted antibiotics in suppressing other coexisting microbial species have been widely recognized, here we discover that such can also serve as redox electron mediators to augment extracellular respiration. Specifically, mitomycin C secreted by Microbacterium proteolyticum ustc, a member Actinobacteria, not only inhibited activity growth...
Cryptosporidium parasites are known to be highly divergent from other apicomplexan species at evolutionary and biological levels. Here we provide evidence showing that the zoonotic parvum also differs apicomplexans, such as Toxoplasma gondii, by possessing only two tubulin-based filamentous structures, rather than an array of subpellicular microtubules. Using affinity-purified polyclonal antibody against C. β-tubulin (CpTubB), observed a long short microtubule rigid stable in sporozoites...
Both exogenous transcriptional factors and chemical-defined medium can transdifferentiate astrocytes into functional neurons. However, the regional preference for such transdifferentiation has not been fully studied. A previously reported 5C was infused mouse cortex striatum to determine from The numbers of NeuN
The use of antibiotics has led to the emergence multidrug-resistant (MDR) bacteria, and there is an urgent need find alternative treatments alleviate this pressure. type VI secretion system (T6SS) a protein delivery present in bacterial cells that secretes effectors participate virulence. Given potential for transformation these into antimicrobial peptides (AMPs), we designed T6SS AMPs have membrane-disrupting effect. These kill bacteria by altering membrane increasing intracellular reactive...
Cryptosporidium parvum is a waterborne and foodborne zoonotic protozoan parasite, causative agent of moderate to severe diarrheal diseases in humans animals. However, fully effective treatments are unavailable for medical veterinary uses. There need explore new drug targets potential development therapeutics. Because C. relies on anaerobic metabolism produce ATP, fermentative enzymes this parasite attractive exploration. In study, we investigated the ethanol-fermentation characterized basic...
Restoring colistin’s efficacy is crucial in addressing the resistance crisis of colistin. This study utilized a high-throughput screening method to identify 43 compounds from 800 FDA-approved drugs that exhibited significant antibacterial effects when combined with Among these, cinacalcet hydrochloride (CH) was selected for its potential synergistic effect colistin against multidrug-resistant (MDR) E. coli strains, including mcr-1-positive strains. A series experiments revealed combination...
The prime purpose of this research currently being done was to set up an acceptable and high-operational ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method, which could simultaneously analyze the concentration levels brexpiprazole, assisted medication for treating depression, DM-3411, principal metabolite brexpiprazole in rats, examine impact baicalin on metabolism rats. After quick sample preparation using acetonitrile as a protein precipitating agent,...
Background: CYP2C19 is an important member of the cytochrome P450 enzyme superfamily. We recently identified 31CYP2C19 alleles in Han Chinese population; studying effects on drug metabolism can help reduce adverse reactions and therapeutic failure. Aim: The aim this study was to assess catalytic activities 31 allelic isoforms their clomipramine vitro. Methods: wild-type 30 variants were expressed insect cells, each variant characterized using as substrate. Reactions performed at 37°C with...
Abstract The zoonotic Cryptosporidium parvum is a global contributor to infantile diarrheal diseases and opportunistic infections in immunocompromised/weakened individuals. Like other apicomplexans, it possesses several specialized secretory organelles, including micronemes, rhoptry dense granules. However, the understanding of cryptosporidial micronemal composition pathway remains limited. Here we report new protein C. , namely thrombospondin repeat domain-containing protein-4 (CpTSP4),...
Phosphoglucomutase 1 (PGM1) catalyzes the conversion between glucose-1-phosphate and glucose-6-phosphate in glycolysis/glucogenesis pathway. PGM1s are typically cytosolic enzymes organisms lacking chloroplasts. However, protozoan Cryptosporidium parasites possess two tandemly duplicated PGM1 genes evolved by a gene duplication after their split from other apicomplexans. Moreover, downstream isoform contains an N-terminal signal peptide, predicting non-cytosolic location. Here we expressed...