A5005602826- CAR-T cell therapy research
- Prostate Cancer Treatment and Research
- Radiopharmaceutical Chemistry and Applications
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Gene expression and cancer classification
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- Epigenetics and DNA Methylation
- Immunotherapy and Immune Responses
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer-related gene regulation
- Metastasis and carcinoma case studies
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Wnt/β-catenin signaling in development and cancer
- Circular RNAs in diseases
- Protease and Inhibitor Mechanisms
- Advanced Biosensing Techniques and Applications
- Ferroptosis and cancer prognosis
- Peptidase Inhibition and Analysis
- Cancer Genomics and Diagnostics
- Genomic variations and chromosomal abnormalities
- Hedgehog Signaling Pathway Studies
Amgen (United States)
2019-2024
Songjiang District Central Hospital
2024
Sana Biotechnology (United States)
2023
Wellstat Biologics (United States)
2022
Sorbonne Université
2019
Pitié-Salpêtrière Hospital
2019
Hôpital Lariboisière
2019
Inserm
2019
Astellas Pharma (United States)
2015
Astellas Pharma (Canada)
2015
Because most potential molecular markers and targets are proteins, proteomic profiling is expected to yield more direct answers functional pharmacological questions than does transcriptional profiling. To aid in such studies, we have developed a protocol for making reverse-phase protein lysate microarrays with larger numbers of spots previously feasible. Our first application these arrays was the 60 human cancer cell lines (NCI-60) used by National Cancer Institute screen compounds...
Epithelial-mesenchymal crosstalk is essential for tissue morphogenesis, but incompletely understood. Postnatal mammary gland development requires epidermal growth factor receptor (EGFR) and its ligand amphiregulin (AREG),which generally must be cleaved from transmembrane form in order to function. As the metalloproteinase ADAM17 can process AREG culture Adam17–/– mice tend phenocopy Egfr–/– mice, we examined role of each these molecules development. Tissue recombination transplantation...
Abstract The mammary gland develops in a process known as branching morphogenesis, whereby distal epithelial bud extends and bifurcates to form an extensive ductal network. Compared with other branched organs, such the lung kidney, little is about molecular basis of gland. Here we report microarray profiling strategy identify novel genes that may regulate branching. We microdissected terminal end (TEB) mature duct microenvironments from β‐actin–green fluorescent protein reporter mice...
Heterotrimeric guanine nucleotide binding proteins (G proteins) transduce extracellular signals received by transmembrane receptors to effector proteins. Each subunit of the G protein complex is encoded a member one three corresponding gene families. Currently, 16 different members alpha family, 5 beta and 11 gamma family have been described in mammals. Here we identified characterized Bacterial Artificial Chromosomes (BACs) containing human homologs each alpha, beta, genes as well alpha11...
Abstract Chromosome rearrangement, a hallmark of cancer, has profound effects on carcinogenesis and tumor phenotype. We used panel 60 human cancer cell lines (the NCI-60) as model system to identify relationships among DNA copy number, mRNA expression level, drug sensitivity. For each 64 cancer-relevant genes, we calculated all 4,096 possible Pearson's correlation coefficients relating number (assessed by comparative genomic hybridization using bacterial artificial chromosome microarrays)...
The Cancer Genome Atlas (TCGA) projects have advanced our understanding of the driver mutations, genetic backgrounds, and key pathways activated across cancer types. Analysis TCGA datasets mostly focused on somatic mutations translocations, with less emphasis placed gene amplifications. Here we describe a bioinformatics screening strategy to identify putative genes amplified datasets. We carried out GISTIC2 analysis spanning 14 subtypes identified 461 that were in two or more list was...
Abstract Purpose: Safety and efficacy of acapatamab, a prostate-specific membrane antigen (PSMA) x CD3 bispecific T-cell engager were evaluated in first-in-human study metastatic castration-resistant prostate cancer (mCRPC). Patients Methods: with mCRPC refractory to androgen receptor pathway inhibitor therapy taxane-based chemotherapy received target acapatamab doses ranging from 0.003 0.9 mg dose exploration (seven levels) 0.3 (recommended phase II dose) expansion intravenously every 2...
The transcription factor ZNF217 is a candidate oncogene in the amplicon on chromosome 20q13 that occurs 20% to 30% of primary human breast cancers and correlates with poor prognosis. We show Znf217 overexpression drives aberrant differentiation signaling events, promotes increased self-renewal capacity, mesenchymal marker expression, motility, metastasis, represses an adult tissue stem cell gene signature downregulated cancers. By silico screening, we identified therapeutics at low...
The study of mammalian development has offered many insights into the molecular aetiology cancer. We previously used analysis mammary morphogenesis to discover a critical role for GATA-3 in developmental and carcinogenesis. In recent years an important microRNAs (miRNAs) myriad cellular processes oncogenesis emerged.
Abstract E-cadherin (E-cad) is a transmembrane adhesion glycoprotein, the expression of which often reduced in invasive or metastatic tumors. To assess E-cad's distribution among different types cancer cells, we used bisulfite-sequencing for detailed, base-by-base measurement CpG methylation promoter region NCI-60 cell lines. The mean levels lines were distributed bimodally, with values pushed toward either high low end scale. 38 epithelial showed substantially lower (28%) compared...
Cancer metastases arise in part from disseminated tumor cells originating the primary and residual disease persisting after therapy. The identification of biomarkers on micro-metastases, tumors, may yield novel tools for early detection treatment these states prior to their development into recurrent tumors. Here we describe molecular profiling lungs, lung metastases, MMTV-PyMT breast cancer model. mice were bred with actin-GFP mice, focal hyperplastic lesions pubertal MMTV-PyMT;actin-GFP...
TPS261 Background: AMG 160 is a novel HLE BiTE immune therapy that redirects T cells to kill tumor by binding PSMA on and CD3 cells. Methods: Primary objectives of this open-label, ascending, multiple-dose, phase 1 study (NCT03792841) are evaluate safety tolerability determine the maximum tolerated dose (MTD) or recommended 2 (RP2D) in men with mCRPC; secondary characterize pharmacokinetics (PK) preliminary efficacy. The exploration will estimate MTD RP2D Bayesian logistic regression...
In mammals, the vomeronasal organ (VNO) contains chemosensory receptor cells that bind to pheromones and induce a variety of social reproductive behaviors. It has been traditionally assumed human VNO (Jacobson's organ) is vestigial structure, although recent studies have shown minor evidence for structurally intact possibly functional VNO. The presence function remains controversial, however, as receptors not well characterized. this study we screened Bacterial Artificial Chromosome (BAC)...
Transforming growth factor (ß1TGFß1) can promote proliferation in late stage cancers but acts as a tumor suppressor normal epithelial cells and early cancers. Although, the TGFß pathway has been shown to play key role tumorigenesis metastasis, only limited number of models have developed understand this process. Here, we present novel model system discern paradoxical TGFß1 using MDA-MB-231 (MB-231) cell line. The MB-231 triple-negative breast cancer line extensively characterized continue...
Abstract Background Monoclonal antibodies are used extensively throughout the biomedical sciences for detection of antigens, either i n vitro or in vivo . We, example, have them quantitation proteins on "reverse-phase" protein lysate arrays. For those studies, we quality-controlled > 600 available monoclonal and also needed to develop precise information genes that encode their antigens. Translation among various gene identifier types proved non-trivial because one-to-many many-to-one...
On p. 3924 of this article in the section `Mammary organoid culture', concentrations three constituents basal medium are incorrect. The correct 10 μg/ml insulin, 5.5 transferrin and 5 ng/ml sodium selenite.In addition, authors also wish to acknowledge Jimmie E. Fata for his suggestions regarding assay.The apologise readers these mistakes.
TPS5088 Background: Lesions in mCRPC are typically immunologically cold. AMG 160 binds to PSMA on cancer cells and CD3 T cells, leading T-cell infiltration, activation, expansion, tumor cell killing. In a first-in-human study, has demonstrated manageable safety profile with preliminary efficacy heavily pretreated patients. Enzalutamide abiraterone novel hormonal therapies (NHTs) that improve survival may enhance responses, but resistance occurs. Combination therapy help overcome broaden use...