A5033050524- Orthopedic Infections and Treatments
- Bacterial biofilms and quorum sensing
- Orthopaedic implants and arthroplasty
- Antimicrobial Resistance in Staphylococcus
- Total Knee Arthroplasty Outcomes
- Bone fractures and treatments
- Tendon Structure and Treatment
- Spine and Intervertebral Disc Pathology
- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Anesthesia and Pain Management
Massachusetts General Hospital
2022-2025
Abstract Background While antibiotics remain our primary tools against microbial infection, increasing antibiotic resistance (inherent and acquired) is a major detriment to their efficacy. A practical approach maintaining or reversing the efficacy of use other commonly used therapeutics, which show synergistic antibacterial action with antibiotics. Here, we investigated extent synergy between gentamicin anti-inflammatory ketorolac regarding dynamics biofilm growth, rate acquired resistance,...
ABSTRACT Periprosthetic joint infection (PJI) is a severe complication of total arthroplasty (TJA), leading to high rates revision surgeries, long‐term morbidity, and mortality. Conventional antibiotic treatments often suffer from limited bioavailability systemic toxicity. This study explores novel approach using vancomycin‐loaded poly(lactic‐ co ‐glycolic) acid (PLGA) microparticles (VMP) formulated via microfluidic double emulsion method for controlled, localized drug delivery managing...
Little is known about the in-vivo dynamics of biofilms associated with medical-device infections and their interplay systemic inflammation, local immune responses, tissue healing processes. There may be an opportunity to tailor therapeutic strategies target these improve treatment outcomes. We investigated responses a methicillin-susceptible (ATCC 12600) multi-drug resistant (L1101) S. aureus strain using rat subcutaneous implant model, analyzing inflammation through 19 gene expressions over...
Abstract Background Arthrofibrosis is a joint disorder characterized by excessive scar formation in the tissues. Vitamin E an antioxidant with potential anti‐fibroblastic effect. The aim of this study was to establish arthrofibrosis rat model after replacement and assess effects vitamin supplementation on fibrosis. Methods We simulated knee 16 male Sprague–Dawley rats. immobilized surgical leg suture full flexion. control groups were killed at 2 12 weeks ( n = 5 per group), test group...
With the advent of antibiotic-eluting polymeric materials for targeting recalcitrant infections, using preclinical models to study biofilms are crucial improving treatment efficacy in periprosthetic joint infections. The stratification risk and severity infections is needed develop an effective clinical dosing framework with better outcomes. We use vivo vitro implant-associated infection demonstrate that methicillin-sensitive resistant Staphylococcus aureus (MSSA MRSA) have model-dependent...
Periprosthetic joint infections occur in about 2% of patients who undergo primary total arthroplasty, a procedure performed over 1 million times the United States. The gold standard treatment is two-stage revision. This study aimed to establish preclinical small animal model (rat) test and compare efficacy an antibiotic-eluting material managing infection. Joint replacement was simulated by transchondylarly implanting polyethylene (PE) plug into distal femur titanium screw proximal tibia....
With the advent of antibiotic-eluting polymeric materials for targeting recalcitrant infections, using preclinical models to study biofilm is crucial improving treatment efficacy in periprosthetic joint infections. The stratification risk and severity infections needed develop an effective clinical dosing framework with better outcomes. Here, in-vivo in-vitro implant-associated infection models, we demonstrate that methicillin-sensitive resistant
Rodent models are commonly employed to validate preclinical disease through the evaluation of postoperative behavior and allodynia. Our study investigates dynamic interplay between pain functional recovery in context traumatic osteotomy surgical repair. Specifically, we established a rat model tibial osteotomy, followed by internal fixation using 5-hole Y-plate with 4 screws, explore hypothesis that histological bone healing is closely associated recovery.Our primary objective was assess...
Abstract Background While antibiotics remain our primary tools against microbial infection, increasing antibiotic resistance (inherent and acquired) is a major detriment to their efficacy. A practical approach maintaining or reversing the efficacy of use other commonly used therapeutics, which show synergistic antibacterial action with antibiotics. Here, we investigated extent synergy between gentamicin anti-inflammatory ketorolac regarding dynamics biofilm growth, rate acquired resistance,...
Abstract Background Postoperative behavior and allodynia evaluation in rodents are commonly used to confirm preclinical disease models. In contrast, we investigating pain functional recovery after traumatic fracture surgical repair. We created a tibial the rat repaired internally with plating. hypothesized that histological bone healing would be strongly correlated recovery. Methods Sixteen male Sprague-Dawley rats underwent metaphyseal transverse osteotomy of proximal tibia. The defect was...