A5057542209- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Biotin and Related Studies
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Luminescence and Fluorescent Materials
- Adipose Tissue and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Sensors
- Peroxisome Proliferator-Activated Receptors
- Cancer, Hypoxia, and Metabolism
- Radiopharmaceutical Chemistry and Applications
- Molecular Junctions and Nanostructures
- Mitochondrial Function and Pathology
- Protein purification and stability
- Pharmacological Effects of Natural Compounds
- Computational Drug Discovery Methods
- Nanoparticle-Based Drug Delivery
- Renal and related cancers
- Supramolecular Self-Assembly in Materials
- Retinoids in leukemia and cellular processes
Kyushu University
2016-2025
Kyoto University
2010-2018
Kyoto Katsura Hospital
2013
Selective protein labeling with a small molecular probe is versatile method for elucidating functions in living cells. In this paper, we report covalent of tag-fused G-protein coupled receptor (GPCR) proteins expressing on cell surfaces utilizing functional molecules. This employs the selective and rapid reaction peptide tag probe, which comprises cysteine-containing short CA6D4x2 (CAAAAAADDDDGDDDD) tetranuclear Zn(II)-DpaTyr containing reactive alpha-chloroacetyl moiety. The GPCRs such as...
Hydropersulfide (R-SSH) is an important class of reactive sulfur species (RSS) involved in a variety physiological processes mammals. A fluorescent probe capable real-time detection hydropersulfide levels living cells would be versatile tool to elucidate its roles cell signalling and redox homeostasis. In this paper, we report ratiometric for sensing, based on fluorescence resonance energy transfer (FRET) mechanism. This sensing mechanism involves nucleophilic reaction with the pyronine-unit...
Detection of metabolic activity enables us to reveal the inherent state cells and elucidate mechanisms underlying cellular homeostasis growth. However, a fluorescence approach for study pathways is still largely unexplored. Herein, we have developed new chemical probe fluorescence-based detection fatty acid β-oxidation (FAO), key process in lipid catabolism, tissues. This serves as substrate FAO forms reactive quinone methide (QM) result reactions. The liberated QM covalently captured by...
Fluorescence imaging of fatty acid beta oxidation (FAO) with a fluorescent probe metabolically degraded by sequential enzyme reactions FAO.
Site-selective cleavage of the peptide backbone in proteins is an important class post-translational modification (PTM) nature. However, organic chemistry for such site-selective bond cleavages has yet to be fully explored. Herein, we report cysteine S-formylation as a means selective protein cleavage. We developed N-formyl sulfonylanilide cysteine-selective formylation reagent peptides and proteins. Upon with reagent, amide adjacent S-formylated cleaved by hydrolysis under neutral aqueous...
A new method for in-cell protein labeling was developed. This employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and Ni(II)–NTA containing an α-chloroacetamide. Using this method, not only of fused proteins but also detection protein–protein interaction achieved inside living cells.
Despite continuous and active development of fluorescent metal-ion probes, their molecular design for ratiometric detection is restricted by the limited choice available sensing mechanisms. Here we present a multicolor platform metal ions based on interaction between ion aromatic ring fluorophore (arene-metal-ion, AM, coordination). Our provided probes possessing 1,9-bis(2'-pyridyl)-2,5,8-triazanonane as flexible binding unit attached to tricyclic fluorophore. This architecture allows sense...
Reported is a new fluorescent probe based on boron-dipyrromethene dye (BODIPY) for late endosome staining in live cells. Among the 14 pH-responsive BODIPY-based far-red/near-infrared dyes used, it was found that (4-methyl-1-piperazinyl)phenyl-substituted BODIPY could stain endosomes three different cell lines. This applicable measurement of fusion time and lysosomes, thereby demonstrating utility this functional analyses
A new glutathione (GSH)-responsive traceless tag that facilitates intracellular delivery of small molecule chemical probes has been developed.
Mitochondrial fatty acid β-oxidation (FAO) plays a key role in energy homeostasis. Several FAO evaluation methods are currently available, but they not necessarily suitable for capturing the dynamics of vivo at cellular-level spatial resolution and seconds-level time resolution. FAOBlue is coumarin-based probe that undergoes to produce fluorescent substrate, 7-hydroxycoumarin-3-(N-(2-hydroxyethyl))-carboxamide (7-HC). After confirming 7-HC could be specifically detected using multiphoton...
Recently, mitochondrial dysfunction has gained attention as a causative factor in the pathogenesis and progression of age-related macular degeneration (AMD). Mitochondrial damage plays key role metabolism disrupts balance intracellular metabolic pathways, such oxidative phosphorylation (OXPHOS) glycolysis. In this study, we focused on oxidized low-density lipoprotein (ox-LDL), major constituent drusen that accumulates retina patients with AMD, investigated whether it could be for alterations...
Electron microscopy (EM) is a technology that enables visualization of single proteins at nanometer resolution. However, current protein analysis by EM mainly relies on immunolabeling with gold-particle-conjugated antibodies, which compromised large size antibody, precluding precise detection location in biological samples. Here, we develop specific chemical labeling method for single-molecular level. Rational design α-helical peptide tag and probe structure provided complementary reaction...
Control of fluorescent dye localization in live cells is crucial for fluorescence imaging. Here, we describe quantitative structure activity relation (QSAR) models predicting intracellular dyes. For generating the QSAR models, electric charge (Z) calculated by pKa, conjugated bond number (CBN), largest fragment (LCF), molecular weight (MW) and log P were used as parameters. We identified 119 BODIPY dyes NIH3T3 cells, assessed accuracy our comparing their predictions with observed...
Chemical labeling of proteins with synthetic molecular probes offers the possibility to probe functions interest in living cells. However, methods for covalently tar...
A peptide recognition strategy based on triple helix hybridization leads to a host–guest system showing high affinity and selectivity in an aqueous medium.
Self-immolative linker is a useful building block of molecular probes, with broad applications in the fields enzyme activity analysis, stimuli-responsive material science, and drug delivery. This manuscript presents N-methyl dimethyl methyl (i.e., trimethyl) carbamate as new class self-immolative for fluorescence detection reactions. The trimethyl was shown to spontaneously undergo intramolecular cyclization upon formation carboxylate group, liberate fluorophore second time rapid reaction...
Abstract Fluorescent probes are indispensable tools for detection of enzymatic activities. However, enzymes with high substrate specificity substrate-based fluorescent is still challenging, due to their relatively large molecular size. We report herein enzyme assays coupled a bioorthogonal fluoride ion-induced desilylation fluorogenic probe. This sensing system useful in situ reactions specificity.
Site-selective cleavage of the peptide backbone in proteins is an important class post-translational modification (PTM) nature. However, organic chemistry for such site-selective bond cleavages has yet to be fully explored. Herein, we report cysteine S-formylation as a means selective protein cleavage. We developed N-formyl sulfonylanilide cysteine-selective formylation reagent proteins. Upon with reagent, amide adjacent S-formylated cleaved by hydrolysis under neutral aqueous conditions....
Hericenone C is one of the most abundant secondary metabolites derived from Hericium erinaceus, under investigation for medicinal properties. Here, we report that inhibits second phase formalin-induced nociceptive behavior in mice. As involved inflammation, a mechanistic analysis on cultured cells targeting NF-κB response element (NRE): luciferase (Luc)-expressing cells, lipopolysaccharide (LPS)-induced NRE::Luc activity was found to be significantly inhibited by C. Phosphorylation p65,...
Increased dietary phosphate consumption intensifies renal burden. Several mechanisms for phosphate-induced tubulointerstitial fibrosis have been reported. Considering the dual nature of as both a potential toxin and an essential nutrient body, kidneys may possess inherent protective against overload, rather than succumbing solely to injury. However, there is limited understanding such mechanisms. To identify these mechanisms, we conducted single-cell RNA sequencing (scRNA-seq) analysis...
Abstract Brown adipocytes are characterized by a high abundance of mitochondria, allowing them to consume fatty acids for heat production. Increasing the number brown is considered promising strategy combating obesity. However, molecular mechanisms underlying their differentiation remain poorly understood. In this study, we demonstrate that etomoxir, an inhibitor Carnitine Palmitoyltransferase 1 (CPT1), inhibits through independent β-oxidation inhibition. presence etomoxir during adipocyte...