A5048457996- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Health and Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- Ovarian function and disorders
- Prenatal Screening and Diagnostics
- Tissue Engineering and Regenerative Medicine
- Reproductive System and Pregnancy
- Pluripotent Stem Cells Research
- Renal and related cancers
- Endometriosis Research and Treatment
- 3D Printing in Biomedical Research
- Seed Germination and Physiology
- Gynecological conditions and treatments
- Ovarian cancer diagnosis and treatment
- Ectopic Pregnancy Diagnosis and Management
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Receptor Mechanisms and Signaling
- Sexual Differentiation and Disorders
- Artificial Intelligence in Healthcare and Education
- Acne and Rosacea Treatments and Effects
- Reproductive Physiology in Livestock
- Generative Adversarial Networks and Image Synthesis
- Animal Genetics and Reproduction
- Genetic Syndromes and Imprinting
Cleveland Clinic
2016-2025
Women’s Health Care
2022-2025
University Hospitals of Cleveland
1997-2024
Bombay Hospital
2016-2023
University of California, Los Angeles
2012-2017
Frauenklinik Heidelberg
2013
Universitäts Frauenklinik
2013
St. Luke's-Roosevelt Hospital Center
2009-2010
Case Western Reserve University
1997-2009
Oakland University
2009
Time-lapse imaging combined with embryo morphokinetics may offer a non-invasive means for improving selection. Data from clinics worldwide are necessary to compare and ultimately develop classifications models using kinetic data. The primary objective of this study was determine if there were differences between embryos limited potential those more often associated in vitro blastocyst formation and/or implantation. We also wanted putative markers selection as proposed by other laboratories...
To determine whether cleavage anomalies, multinucleation, and specific cellular kinetic parameters available from time-lapse imaging are predictive of developmental capacity or blastocyst chromosomal status.Retrospective analysis prospectively collected data.Single academic center.A total 1,478 zygotes patients with blastocysts biopsied for preimplantation genetic screening were cultured in the EmbryoScope.Trophectoderm biopsy.Embryo dysmorphisms, kinetics, euploidy.Of 767 blastocysts, 41.6%...
To identify blastocyst features independently predictive of successful pregnancy and live births with vitrified-warmed blastocysts.Retrospective study.Academic hospital.Women undergoing a cycle transfer blastocysts vitrified using the Rapid-i closed carrier (n = 358).None.Clinical live-birth rates analyzed logistic regression analysis.A total 669 were assessed. The survival rate was 95%. A mean 1.7 ± 0.5 embryos transferred. clinical pregnancy, live-birth, implantation 55%, 46%, 43%,...
Folliculogenesis within the ovary requires interaction between somatic cell components and oocyte. Maintenance of 3-dimensional (3-D) architecture granulosa-oocyte may be critical for successful in vitro maturation follicles. Testing novel biomaterials 3-D culture follicles ultimately lead to a model that can support longer intervals needed human oocytes from ovarian tissue biopsies.A tyramine-based hyaluronan (HA) hydrogel was tested its biocompatibility with The HA prepared at...
Abstract Background High cooling rates with vitrification can be achieved through the use of carriers that allow cryopreservation in fluid volumes < one μl. Open direct contact embryos liquid nitrogen (LN2) whereas closed carrier systems sequester embryo within a sealed system during immersion LN2. The may preferable to reduce possibility cross-contamination. In present study, we compare open and for embryos. We also examine their ability retain viability vapor phase transport. Methods...
Artificial intelligence (AI) models analyzing embryo time-lapse images have been developed to predict the likelihood of pregnancy following in vitro fertilization (IVF). However, limited research exists on methods ensuring AI consistency and reliability clinical settings during its development validation process. We present a methodology for developing validating an model across multiple datasets demonstrate reliable performance evaluating blastocyst-stage embryos. This multicenter analysis...
Abstract Background The insulin-like growth factor 2 (IGF2) gene, a paternally imprinted gene inactive in oocytes, plays vital role early embryo development. While five IGF2 variants have been described, the specific expressed human spermatozoa compared to granulosa cells (GCs) remain unclear. Objective To characterize quantity and of transcripts spermatozoa. Methods Post-gradient sperm samples were collected from two healthy, fertile men with normal semen parameters, while GCs isolated...
Abstract Background Compaction is an important marker of embryonic genome activation and marks a critical step in the development to blastocyst. The objective our study was determine whether visualization compaction process through time-lapse imaging (TL) can assist predicting kinetics embryo as well likelihood for blastocyst formation, grade, or ploidy. Methods This retrospective review prospectively collected data from single academic institution. Couples included were those who underwent...
Sperm play an essential role in embryonic genome activation and progression to blastocyst. In the present work, we focus on development of embryos created as a result ICSI with testicular or epididymal sperm from azoospermic males compare this outcomes normospermic males. The objective study was determine if origin influences clinical outcomes, kinetics embryo development, incidence cleavage anomalies multinucleation.A total 93 consecutive intracytoplasmic injection cycles (ICSI) performed...
To compare different outcomes of vitrification and slow freezing isolated pre-antral follicles to evaluate cryo-devices for follicles.Pre-antral were from mouse ovaries cryopreserved using freezing. A preliminary experiment was carried out select the optimal cryo-device follicles. total 414 randomly distributed among four groups: control (CT) fresh (n=100), nylon mesh (n=96), electron microscopy grid (n=102), micro-capillary tips (n=116). Subsequently, a 979 assigned three CT (n=256),...
Fertilization, cell division and embryo development depend on genomic contributions from male female gametes. We hypothesize that teratozoospermic sperm influences early compaction.We conducted a retrospective analysis of embryos derived intracytoplasmic injection (ICSI) cycles. Two hundred thirty-five consecutive ICSI cycles were included in the study; all treatment was provided at Cleveland Clinic Fertility Center. Patient divided by morphology based Kruger's strict criteria: Group A,...
This study was designed to identify and quantify concentrations of growth factors/cytokines released by Vero cells during the co-culture interval. The factors screened for in this preliminary investigation, namely platelet-derived factor (PDGF), transforming beta (TGFbeta), interleukin-6 (IL-6), leukaemia inhibitory (LIF) epidermal (EGF) have each been identified impact on early embryo development or are secreted embryos themselves, suggesting an autocrine regulatory role. cell culture...
We examined the effects of varicocelectomy on semen parameters in 30 subfertile men, with emphasis potential changes sperm count, motility and morphology as measured by Kruger's strict morphologic criteria.A total patients underwent subinguinal (25 bilateral 5 unilateral). Preoperative postoperative density, were analyzed. follicle-stimulating hormone, luteinizing hormone testosterone levels compared to those fertile volunteers enrolled our donation program. Pregnancy rates after also...