A5020500235- Virology and Viral Diseases
- Genomics and Rare Diseases
- Respiratory viral infections research
- Poxvirus research and outbreaks
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Genomic variations and chromosomal abnormalities
- BRCA gene mutations in cancer
- Immunotherapy and Immune Responses
- Genomics and Phylogenetic Studies
- Prenatal Screening and Diagnostics
- Cancer Genomics and Diagnostics
- Syphilis Diagnosis and Treatment
- vaccines and immunoinformatics approaches
- Herpesvirus Infections and Treatments
- Plant Virus Research Studies
- Nutrition, Genetics, and Disease
- Bacteriophages and microbial interactions
- Ethics in Clinical Research
- Neurogenetic and Muscular Disorders Research
- Genetics, Bioinformatics, and Biomedical Research
- Fetal and Pediatric Neurological Disorders
- Rabies epidemiology and control
HudsonAlpha Institute for Biotechnology
2020-2024
Centers for Disease Control and Prevention
2010-2024
National Center for Immunization and Respiratory Diseases
2010-2024
Emory University
2004-2013
Office of Health and Safety
2010
Northeastern University
2010
Massachusetts Department of Public Health
2010
University of Florida
2000-2007
Long-lived plasma cells that reside in the bone marrow constitutively produce antibody absence of antigen and are cellular basis durable humoral immunity. The generation these long-lived depends upon a series highly orchestrated interactions between antigen-specific CD4 T B formation germinal centers (GCs). In this study, we have examined role cytokine interleukin-21 (IL-21) regulating immunity during acute viral infections. Using IL-21 receptor-deficient (IL-21R(-/-)) mice, found...
Variant detection from long-read genome sequencing (lrGS) has proven to be more accurate and comprehensive than variant short-read (srGS). However, the rate at which lrGS can increase molecular diagnostic yield for rare disease is not yet precisely characterized. We performed using Pacific Biosciences HiFi technology on 96 short-read-negative probands with diseases that were suspected genetic. generated hg38-aligned variants de novo phased assemblies, subsequently annotated, filtered,...
CD4(+) T follicular helper (TFH) cells are central for generation of long-term B-cell immunity. A defining phenotypic attribute TFH is the expression chemokine R CXCR5, and typically identified by co-expression CXCR5 together with other markers such as PD-1, ICOS, Bcl-6. Herein, we report high-level nutrient transporter folate 4 (FR4) on in acute viral infection. Distinct from profile conventional markers, FR4 was highly expressed naive cells, downregulated after activation subsequently...
ABSTRACT Although high measles, mumps, and rubella (MMR) vaccination coverage has been successful in dramatically reducing mumps disease the United States, (re)infections occasionally occur individuals who have either previously vaccinated or naturally infected. Standard diagnostics that detect virus virus-specific antibody are dependable for confirming primary infection immunologically naïve persons, but these methods perform inconsistently with prior immune exposure. We hypothesized...
ABSTRACT Neutralizing antibodies are assumed to be essential for protection against mumps virus infection, but their measurement is labor- and time-intensive. For this reason, enzyme-linked immunosorbent assays (ELISAs) typically used measure mumps-specific IgG levels. However, since there poor correlation between neutralization titers ELISAs that the presence of levels, better correlate with needed. To address issue, we measured antibody levels by plaque reduction neutralization, a...
It is critical to understand the wide-ranging clinical and non-clinical effects of genome sequencing (GS) for parents in NICU context. We assessed parents' experiences with GS as a first-line diagnostic tool infants suspected genetic conditions NICU.Parents newborns (N = 62) having condition were recruited across five hospitals southeast United States part SouthSeq study. Semi-structured interviews 78) conducted after received their child's result (positive, negative, or variants unknown...
Variant detection from long-read genome sequencing (lrGS) has proven to be considerably more accurate and comprehensive than variant short-read (srGS). However, the rate at which lrGS can increase molecular diagnostic yield for rare disease is not yet precisely characterized. We performed using Pacific Biosciences "HiFi" technology on 96 short-read-negative probands with that were suspected genetic. generated hg38-aligned variants de novo phased assemblies, subsequently annotated, filtered,...
Recent mumps outbreaks among 2-dose measles rubella (MMR) vaccine recipients have raised questions regarding the potential benefits of a third dose (MMR3). If MMR3 provides sustained elevation in antibody, it may be beneficial for certain at-risk groups or as an outbreak control measure.Sera were collected immediately prior to and at 1 month year post-MMR3 from 656 healthy adults aged 18-28 years nonoutbreak setting. Immunoglobulin G (IgG) was measured by enzyme-linked immunosorbent assay...
Measurement of measles virus-specific IgG is used to assess presumptive evidence immunity among immunocompetent individuals with uncertain immune or vaccination status. False-negative test results may lead unnecessary quarantine and exclusion from activities such as employment, education, travel result in revaccination. In contrast, false-positive fail identify susceptible promote spread disease by those who are exposed unprotected. To better understand the performance characteristics tests...
ABSTRACT Unlike naive CD8 + T cells, antigen-experienced memory cells persist over time due to their unique ability homeostatically proliferate. It was hypothesized that might differentially regulate the expression of genes control cell cycle facilitate homeostatic proliferation. To test this, levels 96 different regulatory were compared between transgenic and specifically recognize GP33-41 epitope lymphocytic choriomeningitis virus (LCMV). discovered relative overexpress several important...
Laboratory confirmation of infection is an essential component measles surveillance. Detection measles-specific IgM in serum by enzyme-linked immunosorbent assay (ELISA) the most common method used to confirm infection. ELISA formats vary, as does sensitivity and specificity each assay. Specimens collected within 3 days rash onset can yield a false-negative result, which delay cases. Interfering substances false-positive leading unnecessary public health interventions. The capture developed...
Neurodevelopmental disorders (NDDs) often result from rare genetic variation, but genomic testing yield for NDDs remains around 50%, suggesting some clinically relevant variants may be missed by standard analyses. Here we analyze "poison exons" (PEs) which, while absent gene annotations, are alternative exons whose inclusion results in a premature termination codon. Variants that alter PE can lead to loss-of-function and highly penetrant contributors disease.
Among our cohort of adolescents and young adults with perinatally acquired human immunodeficiency virus, few (17.6%) had measles protective antibodies by plaque reduction neutralization (PRN). Agreement was demonstrated between the commercial enzyme immunoassay PRN assay (K = 0.59 [95% confidence interval: 0.23–0.95]). Further studies are needed to understand determinants immunity in this population.