A5044902025- Poxvirus research and outbreaks
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Herpesvirus Infections and Treatments
- Plant Virus Research Studies
- RNA Research and Splicing
- Biochemical and Molecular Research
- RNA modifications and cancer
- Mosquito-borne diseases and control
- Toxin Mechanisms and Immunotoxins
- Bacillus and Francisella bacterial research
- Cell Adhesion Molecules Research
- RNA regulation and disease
- Tissue Engineering and Regenerative Medicine
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Cancer-related gene regulation
- Enzyme Structure and Function
- Plant and Fungal Interactions Research
- Advanced Proteomics Techniques and Applications
- Cancer Cells and Metastasis
- Malaria Research and Control
- Trypanosoma species research and implications
- Mass Spectrometry Techniques and Applications
University of California, Irvine
2014-2024
Edwards Lifesciences (United States)
2013
Texas A&M University
1995-2004
Texas A&M University System
2000-2004
University of Houston
2004
Baylor College of Medicine
1998-2002
Howard Hughes Medical Institute
1998-2002
Rice University
2001
National Institute of Allergy and Infectious Diseases
1990-1994
National Institutes of Health
1990-1994
A role for fibroblasts in physiological and pathological angiogenesis is now well recognized; however, the precise mechanisms underlying their action have not been determined. Using an vitro model combination with a candidate gene approach, column chromatography, mass spectrometry, we identify two classes of fibroblast-derived factors--one that supports vessel sprouting but lumen formation, one promotes formation. In absence angiopoietin-1, angiogenin, hepatocyte growth factor, transforming...
Pluripotent stem cell-derived cardiomyocytes (CMs) have great potential in the development of new therapies for cardiovascular disease. In particular, human induced pluripotent cells (iPSCs) may prove especially advantageous due to their pluripotency, self-renewal potential, and ability create patient-specific cell lines. Unfortunately, CMs are immature, with characteristics more closely resembling fetal than adult CMs, this immaturity has limited use drug screening cell-based therapies....
Somatic G17V RHOA mutations were found in 50–70% of angioimmunoblastic T-cell lymphoma (AITL). The mutant lacks GTP binding capacity, suggesting defects the classical signaling. Here, we discovered novel function RHOA: VAV1 was identified as a RHOA-specific partner via high-throughput screening. We that to augmented its adaptor through phosphorylation 174Tyr, resulting acceleration receptor (TCR) Enrichment cytokine and chemokine-related pathways also evident by expression RHOA. further new...
The shot noise limitation as well other factors that influence the sensitivity of measurements with a surface plasmon resonance (SPR) sensor are considered. It is demonstrated minute changes in refractive index medium close to metal film can be detected owing shift angle. In particular, adsorption layer only fraction biomolecular monolayer could measured. Data for SPRare presented adjacent media air, water, aqueous solutions ethanol and sodium chloride at different concentrations....
A previously described mammalian cell activity, called VPg unlinkase, specifically cleaves a unique protein–RNA covalent linkage generated during the viral genomic RNA replication steps of picornavirus infection. For over three decades, identity this cellular activity and its normal role in uninfected had remained elusive. Here we report purification identification unlinkase as DNA repair enzyme, 5′-tyrosyl–DNA phosphodiesterase-2 (TDP2). Our data show that different lines correlates with...
Mosquito eggshells show remarkable diversity in physical properties and structure consistent with adaptations to the wide variety of environments exploited by these insects. We applied proteomic, transcriptomic, hybridization situ techniques identify gene products pathways that participate assembly Aedes aegypti eggshell. population density is low during cold dry seasons increases immediately after rainfall. The survival embryos through unfavorable periods a key factor persistence their...
The vaccinia virus gene for S-adenosyl-L-methionine:mRNA (nucleoside-O2'-)-methyltransferase, an enzyme required the formation of 5' cap structure mRNA, was identified. Protein sequence analysis revealed that this cap-specific methyltransferase is derived from same open reading frame as previously shown to encode VP39, a Mr 39,000 dissociable subunit poly(A) polymerase stimulates long tails. Consistent with finding, activity associated heterodimeric polymerase, which composed VP55 and VP39...
The vaccinia virus early transcription factor (VETF) was shown to be a virus-encoded heterodimer. gene for the 82-kDa subunit identified as open reading frame (ORF) A8L, based on N-terminal sequence of purified by using DNA-affinity magnetic beads. 70-kDa VETF refractory analysis, and so sequences were obtained three internal tryptic peptides. All peptides matched within ORF D6R. ORFs A8L D6R are located central region genome separated about 13,600 base pairs. Proteins corresponding 3' ends...
Eucaryotic transcription factors that stimulate RNA polymerase II by increasing the efficiency of elongation specifically or randomly initiated chains have been isolated and characterized. We identified a 30-kilodalton (kDa) vaccinia virus-encoded protein with apparent homology to SII, 34-kDa mammalian transcriptional factor. In addition amino acid sequence similarities, both proteins contain C-terminal putative zinc finger domains. Identification gene, rpo30, encoding virus was achieved...
Due to the limited coding capacity of picornavirus genomic RNAs, host RNA binding proteins play essential roles during viral translation and replication. Here we describe experiments suggesting that AUF1, a protein involved in mRNA decay, plays role infectious cycle picornaviruses such as poliovirus human rhinovirus. We observed cleavage AUF1 or rhinovirus infection, well interaction this with 5' noncoding regions these genomes. Additionally, proteinase 3CD, encoded by was shown cleave all...
We have determined, by high resolution x-ray analysis, 10 structures comprising the mRNA cap-specific methyltransferase VP39 or specific mutants thereof in presence of methylated nucleobase analogs (N1-methyladenine, N3-methyladenine, N1-methylcytosine, N3-methylcytosine) and their unmethylated counterparts, nucleoside N7-methylguanosine. Together with solution affinity studies previous crystallographic data for N7-methylguanosine its phosphorylated derivatives, these demonstrate that only...
ABSTRACT Vaccinia virus, the basis of smallpox vaccine, is one largest viruses to replicate in humans. We have used situ atomic force microscopy (AFM) directly visualize fully hydrated, intact intracellular mature vaccinia virus (IMV) virions and chemical enzymatic treatment products thereof. The latter included virion cores, core-enveloping coats, core substructures. isolated coats appeared be composed a highly cross-linked protein array. AFM imaging substructures indicated association...
Trypsin, Lys-C, and Lys-N are the most broadly used enzymes in proteomics. Here, on basis of large-scale peptide mass spectrometry (MS) data sets, an approach is described to confidently identify missed cleavage sites either phosphorylated or unmodified substrates for these three proteases, any protease, side chain species present within 15 residues cleavage-specificity residue. Previously known effects proline, negatively charged chains, phospho-modified have been quantified, additional...
Angiogenesis requires tightly coordinated crosstalk between endothelial cells (ECs) and stromal cells, such as fibroblasts smooth muscle cells. The specific molecular mechanisms moderating this process are still poorly understood.Stromal cell-derived factors essential for EC sprouting lumen formation. We therefore compared the abilities of 2 primary fibroblast isolates a cell isolate to promote in vitro angiogenesis, analyzed their secretomes using combination nano liquid chromatography-mass...
The mitochondrial and chloroplast mRNAs of the majority land plants are modified through cytidine to uridine (C-to-U) RNA editing. Previously, forward reverse genetic screens demonstrated a requirement for pentatricopeptide repeat (PPR) proteins Moreover, editing factors OZ1, RIP2, RIP9 ORRM1 were identified in co-immunoprecipitation (co-IP) experiments, albeit minimal complex sufficient activity was never deduced. current study focuses on isolated, intact complexes that capable distinct...
We describe the synthesis of a chain-terminating mRNA cap dinucleotide and its use in vitro transcription homogeneously capped RNA. Computer modeling strongly indicates that RNA with new compound will be substrate for cap-dependent translation.