A5031029743- Bacterial Infections and Vaccines
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Pneumonia and Respiratory Infections
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Molecular Junctions and Nanostructures
Cornell University
2023
University of New Mexico
2019
Abstract Engineered outer membrane vesicles (OMVs) derived from Gram-negative bacteria are a promising technology for the creation of non-infectious, nanoparticle vaccines against diverse pathogens. However, antigen display on OMVs can be difficult to control and highly variable due bottlenecks in protein expression localization host cell, especially bulky and/or complex antigens. Here, we describe universal approach avidin-based vaccine crosslinking (AvidVax) whereby biotinylated antigens...
Physical isolation of molecular computing elements holds the potential for increasing system complexity by enabling reuse standardized components and protecting from environmental degradation. However, once have been compartmentalized, methods communicating into these compartments are needed. We report compartmentalization steroid-responsive DNA aptamers within giant unilamellar vesicles (GUVs) that permeable to steroid inputs. Monodisperse GUVs loaded with using a microfluidic platform....