A5010816229- Bacteriophages and microbial interactions
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Bacillus and Francisella bacterial research
- Escherichia coli research studies
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- Carbohydrate Chemistry and Synthesis
- Zoonotic diseases and public health
- Viral Infections and Outbreaks Research
- Proteins in Food Systems
- Infection Control and Ventilation
- T-cell and B-cell Immunology
- Pickering emulsions and particle stabilization
- Salmonella and Campylobacter epidemiology
- Surfactants and Colloidal Systems
- COVID-19 Pandemic Impacts
- COVID-19 epidemiological studies
- Public Health Policies and Education
United States Naval Medical Research Unit SOUTH
2024
Cornell University
2016-2023
University of Pennsylvania
2014
Abstract Engineered outer membrane vesicles (OMVs) derived from Gram-negative bacteria are a promising technology for the creation of non-infectious, nanoparticle vaccines against diverse pathogens. However, antigen display on OMVs can be difficult to control and highly variable due bottlenecks in protein expression localization host cell, especially bulky and/or complex antigens. Here, we describe universal approach avidin-based vaccine crosslinking (AvidVax) whereby biotinylated antigens...
Significance Conjugate vaccines have proven to be an effective and safe strategy for reducing the incidence of disease caused by bacterial pathogens. However, manufacture these is technically demanding, inefficient, expensive, thereby limiting their widespread use. Here, we describe alternative methodology generating glycoconjugate whereby recombinant polysaccharide biosynthesis coordinated with vesicle formation in nonpathogenic Escherichia coli , resulting glycosylated outer membrane...
Conjugate vaccines are among the most effective methods for preventing bacterial infections. However, existing manufacturing approaches limit access to conjugate due centralized production and cold chain distribution requirements. To address these limitations, we developed a modular technology in vitro vaccine expression (iVAX) portable, freeze-dried lysates from detoxified, nonpathogenic Escherichia coli. Upon rehydration, iVAX reactions synthesize clinically relevant doses of against...
Significance A broad range of bacterial, fungal, and protozoan cells produce the surface polysaccharide poly- N -acetyl- d -glucosamine (PNAG), making this antigen an attractive target for vaccination against multiple human economically important animal pathogens. While conjugate vaccines involving polysaccharides, such as PNAG, are a proven strategy reducing incidence disease caused by bacterial pathogens, their manufacture is technically demanding, inefficient, expensive, thereby limiting...
Glycoengineered bacteria have emerged as a cost-effective platform for rapid and controllable biosynthesis of designer conjugate vaccines. However, little is known about the engagement such conjugates with naı̈ve B cells to induce formation germinal centers (GC), subanatomical microenvironment that converts into antibody-secreting plasma cells. Using three-dimensional biomaterials-based B-cell follicular organoid system, we demonstrate triggered robust expression hallmark GC markers, cell...
An emerging field in biomaterials is the creation and engineering of protein surfactants made by recombinant biotechnology. Protein biotechnology allow for complete control molecular weight chemical sequence surfactant. The proteins are monodisperse weight, functionalization with bioactive amino acid sequences straightforwardly achieved through genetic engineering. We modified naturally occurring amphiphilic plant oleosin truncating a large portion its central hydrophobic block, creating...
Significance Asparagine-linked ( N -linked) protein glycosylation—the covalent attachment of complex sugars to the nitrogen atom in asparagine side chains—is most widespread posttranslational modification proteins and also complex. N- glycosylation affects a significant number cellular can have profound effects on their important attributes such as biological activity, chemical solubility, folding stability, immunogenicity, serum half-life. Accordingly, strategic installation glycans at...
Summary Conjugate vaccines are among the most effective methods for preventing bacterial infections, representing a promising strategy to combat drug-resistant pathogens. However, existing manufacturing approaches limit access conjugate due centralized production and cold chain distribution requirements. To address these limitations, we developed modular technology i n vitro bioconjugate va ccine e x pression (iVAX) in portable, freeze-dried lysates from detoxified, nonpathogenic Escherichia...
Characterizing the epidemiology of circulating respiratory pathogens during COVID-19 pandemic could clarify burden acute infections and monitor outbreaks public health military relevance. The US Department Defense supported 2 regions for influenza-like illness severe surveillance, one in Middle East through Naval Medical Research Unit EURAFCENT, another Latin America SOUTH. During 2020‒2022, coinciding with pandemic, we collected a total 16,146 nasopharyngeal oropharyngeal swab samples from...
Infectious diseases (IDs) contribute to major causes of mortality and chronic morbidity in Central South America. Interest improving general understanding, prevention, treatment options motivates the U.S. Department Defense establish run overseas ID institutions enhance force health protection. One 6 institutions, Naval Research Medical Unit (NAMRU) SOUTH, has safely ethically conducted biomedical research, surveillance, medical countermeasure testing evaluation Peru surrounding countries....
Abstract As a common protein modification, asparagine-linked ( N- linked) glycosylation has the capacity to greatly influence biological and biophysical properties of proteins. However, routine use at naïve sites as strategy for engineering proteins with advantageous is currently limited by our inability construct large collections glycoproteins interrogating structural functional consequences glycan installation. To address this challenge, we describe combinatorial termed shotgun scanning...
Abstract Engineered outer membrane vesicles (OMVs) derived from laboratory strains of bacteria are a promising technology for the creation non-infectious, nanoparticle vaccines against diverse pathogens. As mimics bacterial cell surface, OMVs offer molecularly-defined architecture programming repetitive, high-density display heterologous antigens in conformations that elicit strong B and T immune responses. However, antigen on surface can be difficult to control highly variable due...