A5044201568- Nanoparticle-Based Drug Delivery
- Glycosylation and Glycoproteins Research
- Pickering emulsions and particle stabilization
- Dendrimers and Hyperbranched Polymers
- Connective tissue disorders research
- Proteins in Food Systems
- Aortic aneurysm repair treatments
- Surfactants and Colloidal Systems
- Innovative Microfluidic and Catalytic Techniques Innovation
- Micro and Nano Robotics
- Molecular Sensors and Ion Detection
- Monoclonal and Polyclonal Antibodies Research
- Hemoglobin structure and function
- Fuel Cells and Related Materials
- Membrane Separation and Gas Transport
- Fluid Dynamics and Mixing
- RNA Interference and Gene Delivery
- Dyeing and Modifying Textile Fibers
- Diatoms and Algae Research
- Ultrasound and Hyperthermia Applications
- Supramolecular Chemistry and Complexes
- Advanced Polymer Synthesis and Characterization
- Chemical Synthesis and Analysis
- Protein purification and stability
- Olfactory and Sensory Function Studies
University of Pennsylvania
2012-2024
Janssen (United States)
2020
Microbial ID (United States)
2018
The modular synthesis of 7 libraries containing 51 self-assembling amphiphilic Janus dendrimers with the monosaccharides d-mannose and d-galactose disaccharide d-lactose in their hydrophilic part is reported. These unprecedented sugar-containing are named glycodendrimers. Their self-assembly by simple injection THF or ethanol solution into water buffer hydration was analyzed a combination methods including dynamic light scattering, confocal microscopy, cryogenic transmission electron Fourier...
Nanoparticles on demand: Upon the site-specific covalent attachment of hydrophobic molecules to one end biopolymer backbone, chimeric polypeptides (derived from elastin-like polypeptides) can self-assemble form thermoresponsive nanoparticles suitable for drug delivery. Molecules with a distribution coefficient greater than 1.5 imparted sufficient amphiphilicity drive self-assembly into sub-100 nm (see picture). As service our authors and readers, this journal provides supporting information...
Using recombinant amphiphilic proteins to self-assemble suprastructures would allow precise control over surfactant chemistry and the facile incorporation of biological functionality. We used cryo-TEM confirm self-assembled structures from recombinantly produced mutants naturally occurring sunflower protein, oleosin. studied phase behavior protein self-assembly as a function solution ionic strength hydrophilic fraction, observing nanometric fibers, sheets, vesicles. Vesicle membrane...
Elastin-like polypeptides (ELPs) are a class of biopolymers consisting the pentameric repeat (VPGαG)n based on sequence mammalian tropoelastin that display thermally induced soluble-to-insoluble phase transition in aqueous solution. We have discovered remarkably simple approach to driving spontaneous self-assembly high molecular weight ELPs into nanostructures by genetically fusing short 1.5 kDa (XGy)z assembly domain one end ELP. Classical theories geometric mass balance hydrophilic and...
Biological drug products are formulated with excipients to maintain stability over the shelf life of product. Surfactants added product stabilize air–water interfaces known induce protein aggregation. Early formulation development is focused on maintaining conformation and colloidal course but rarely considers through dose preparation administration. Specifically, intravenous (IV) bag exposes therapeutic a different solution environment concurrently diluting stabilizing that had been...
Abstract In this study, we demonstrate the fabrication of polymersomes, protein-blended and polymeric microcapsules using droplet microfluidics. Polymersomes with uniform, single bilayers controlled diameters are assembled from water-in-oil-in-water double-emulsion droplets. This technique relies on adjusting interfacial energies to completely separate polymer-stabilized inner core oil shell. Protein-blended polymersomes prepared by dissolving protein in outer phases Cell-sized size...
Recombinant surfactants present a new platform for stabilizing and targeting nanoparticle imaging agents. Superparamagnetic iron oxide nanoparticle-loaded micelles MRI contrast are stabilized by an engineered variant of the naturally occurring protein oleosin targeted using Her2/neu affibody-oleosin fusion. The recombinant allows simple ability to easily swap ligand numerous targets.
Microbubbles are used as contrast enhancing agents in ultrasound sonography and more recently have shown great potential theranostic that enable both diagnostics therapy. Conventional production methods lead to highly polydisperse microbubbles, which compromise the effectiveness of imaging Stabilizing microbubbles with surfactant molecules can impart functionality properties desirable for specific applications would enhance utility microbubbles. Here we generate monodisperse a large...
An emerging field in biomaterials is the creation and engineering of protein surfactants made by recombinant biotechnology. Protein biotechnology allow for complete control molecular weight chemical sequence surfactant. The proteins are monodisperse weight, functionalization with bioactive amino acid sequences straightforwardly achieved through genetic engineering. We modified naturally occurring amphiphilic plant oleosin truncating a large portion its central hydrophobic block, creating...
Targeting nanoparticles for drug delivery has great potential improving efficacy and reducing side effects from systemic toxicity. New developments in the assembly of materials afford opportunity to expose cryptic targeting domains tissue‐specific microenvironments which certain proteases are expressed. Here, recombinant proteins designed combine responsiveness environmental with specific targeting. Materials made recombinantly allow complete control over amino acid sequence, each molecule...
Polymersomes are bilayer vesicles that self-assemble from amphiphilic diblock copolymers, and provide an attractive system for the delivery of biological nonbiological molecules due to their environmental compatibility, mechanical stability, synthetic tunability, large aqueous core, hyperthick hydrophobic membrane. Herein, we report a nanoscale photoresponsive polymersome featuring meso-to-meso ethyne-bridged bis[(porphinato)zinc] (PZn2) fluorophore membrane solute dextran in core. Upon 488...
Odor poses a growing concern in clothing and apparel applications due to laundering limitations at managing odor-causing microorganisms. Herein, novel silver-polymer complex was applied textile materials studied using quantitative antimicrobial assays, gas chromatography techniques, odor panel sensory tests ascertain control function effectiveness. A known chemical pathway involving leucine conversion isovaleric acid found be disrupted silver-treated fabrics. Furthermore, its absorption...
Chimäre Polypeptide mit einem kovalent gebundenen hydrophoben Molekül an Ende der Biopolymerkette können zu thermoresponsiven Nanopartikeln zusammenlagern, die als Wirkstofftransportsysteme geeignet sind. Moleküle Verteilungskoeffizienten größer 1.5 verleihen den Polymeren genügend Amphiphilie, um deren Selbstorganisation Größen unter 100 nm bewirken (siehe Bild). As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are...