A5014023492- Glycosylation and Glycoproteins Research
- Machine Learning in Bioinformatics
- Chemical Synthesis and Analysis
- Health and Lifestyle Studies
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Protein purification and stability
- Biosensors and Analytical Detection
- Facial Trauma and Fracture Management
- Microbial Metabolism and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Agricultural and Food Production Studies
- Enzyme-mediated dye degradation
- Traumatic Ocular and Foreign Body Injuries
- Surface Modification and Superhydrophobicity
- Peptidase Inhibition and Analysis
- Medication Adherence and Compliance
- Microfluidic and Bio-sensing Technologies
- Electrochemical sensors and biosensors
- TiO2 Photocatalysis and Solar Cells
- Graphene and Nanomaterials Applications
- Cytomegalovirus and herpesvirus research
- CAR-T cell therapy research
- Nanoparticles: synthesis and applications
- Organic Light-Emitting Diodes Research
Cornell University
2019-2025
Industrial University of Santander
2010-2021
Universidad de Los Andes
2016-2020
Instituto de Salud de Bucaramanga
2010
Abstract Protein-protein interactions (PPIs) are critical for biological processes and predicting the sites of these is useful both computational experimental applications. We present a S tructure- gnostic L anguage T ransformer Pep tide Pr ioritization (SaLT&PepPr) pipeline to predict interaction interfaces from protein sequence alone subsequent generation peptidic binding motifs. Our model fine-tunes ESM-2 language (pLM) with per-position prediction task identify PPI using data PDB,...
Therapeutic drugs for Alzheimer's disease have been extensively studied due to its recurrence and abundance among neurodegenerative diseases. It is thought that the accumulation of amyloid precursor protein (APP) products, a consequence an up-regulation β-site APP-cleaving enzyme 1 (BACE1), main triggering mechanism during early stages disease. This study aims explore ability multifunctional conjugate based on magnetite nanoparticles cellular delivery siRNA against expression
ABSTRACT Monoclonal antibodies (mAbs) that specifically recognize cell surface glycans associated with cancer and infectious disease hold tremendous value for both basic research clinical applications. However, high-quality anti-glycan mAbs, especially those sufficiently high affinity specificity, remain scarce, highlighting the need protein engineering approaches based on rational design or directed evolution enable optimization of antigen-binding properties. To this end, we sought to...
Outer membrane protein A (OmpA) has been extensively studied in Gram-negative bacteria due to its relevance the adhesion of pathogens host cells and surfactant capabilities. It consists a hydrophobic β-barrel domain hydrophilic periplasmic domain, that confers OmpA an amphiphilic structure. This study aims elucidate capacity Escherichia coli translocate liposomal membranes serve as potential cell-penetrating vehicle. We immobilized on magnetite nanoparticles investigated possible functional...
Glycoengineered bacteria have emerged as a cost-effective platform for rapid and controllable biosynthesis of designer conjugate vaccines. However, little is known about the engagement such conjugates with naı̈ve B cells to induce formation germinal centers (GC), subanatomical microenvironment that converts into antibody-secreting plasma cells. Using three-dimensional biomaterials-based B-cell follicular organoid system, we demonstrate triggered robust expression hallmark GC markers, cell...
Removal of azo and diazo dye content from textile industry wastewaters is crucial due to their environmental impact. Here, we report on the use fungal laccase Pycnoporus sanguineus CS43 immobilized silica nanoparticles entrapped in textile-based filters for degradation Congo Red. Laccase immobilization synthesis nanocomposites were carried out by two different methods, one presence acetone second using water as solvent. This led a change hydrophobicity obtained biofilters. Successful...
Here we describe a facile and robust genetic selection for isolating full-length IgG antibodies from combinatorial libraries expressed in the cytoplasm of redox-engineered Escherichia coli cells. The method is based on transport bifunctional substrate comprised an antigen fused to chloramphenicol acetyltransferase, which allows positive bacterial cells co-expressing cytoplasmic IgGs called cyclonals that specifically capture chimeric sequester antibiotic resistance marker cytoplasm. utility...
Polymeric microcapsules with the fungal laccase from Pycnoporus sanguineus CS43 may represent an attractive avenue for removal or degradation of dyes wastewaters. Microcapsules alginate/chitosan (9.23 ± 0.12 µm) and poly(styrenesulfonate) (PSS) (9.25 0.35 were synthesized subsequently tested catalytic activity in decolorization diazo dye Congo Red. Successful encapsulation into materials was verified via confocal microscopy labeled enzyme molecules. Laccase measured as a function time...
Abstract Background Eye injury is a serious worldwide public health problem that may cause blindness. In children, blindness has functional impact and psychosocial implications. As indicated in many studies, identification of risk factors associated with the socio-cultural context prevent eye injuries. The objetive study to describe sociodemographic epidemiological characteristics pediatric its effects on ocular structures hospital from Colombia. Method A retrospective cross-sectional was...
Abstract Here, we integrate fine-tuned protein language models and protein-protein interaction databases to develop a Structure-agnostic Language Transformer & Peptide Prioritization module that efficiently selects peptides from interfaces, without the need for structural information. We experimentally fuse SaLT&PepPr-derived “guide” E3 ubiquitin ligase domains reliably identify candidates induce robust intracellular degradation of clinically-relevant targets, exhibit high binding...
Polymeric nanocomposites have been in the scope of scientists for last decade due to their multiple applications and simple synthesis. Self-assembly fabrication can be performed through different methods such as layer-by-layer or controlled growth nanostructures on a surface. These allow fast elaboration that readily integrated sensors films. The current work exposes self-assembly modification material's macroscopic properties hydrophobicity temperature's resistance textiles. Hydrophobicity...
The use of a new copper(I) coordination polymer (CP) as additive in transparent composite films 190 nm thickness for ultraviolet (UV) shielding is presented. luminescent 1-D Cu(I) CP was easily synthesized through self-assembly process between iodide and 2,6-bis(1H-benzotriazol-1-ylmethyl)pyridine (L). CP, [Cu2(μ − I)2(μ L)2]n, structurally characterized by infrared, UV–visible diffuse reflectance photoluminescence spectroscopy, elemental thermogravimetric analyses, single-crystal powder...
Abstract Protein silencing is an important aspect of both scientific investigation native protein function and therapeutic targeting aberrant activity. Many techniques for proteins at the DNA or RNA level exist such as CRISPR, RNAi, TALEN. Cellular can also be selectively removed posttranslational using proteome editing techniques, many which employ engineered to engage natural cellular quality control machinery accelerating removal otherwise stable proteins. Here, we summarize recent...
Introduction: In nursing, art and science of caring, is important to the use nursing process using a standardized language. Therefore, having valid results labels, applied pre pos intervention, allows professional evaluate demonstrate impact such interventions. Especially in management common problems adult population as high blood pressure. Objective: Determine construct validity result "therapeutic conduct: illness or injury" (classification NOC 1609) measure adherence treatment....
Abstract Background Eye injury is a serious public health problem being in the world, that causes unilateral blindness, particular condition children due to functional impact and psychosocial implications. The eye can be prevented thanks identification of risk factors associated with socio-cultural context, this aspect has motivated studies pediatric population United States, India, Australia, Cuba, Egypt, Kingdom, Brazil, Colombia, Marathwada Canada, among others. So objetive present study...