A5103009050- Biofuel production and bioconversion
- Microbial Metabolic Engineering and Bioproduction
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Anaerobic Digestion and Biogas Production
- Biochemical and biochemical processes
- Fungal and yeast genetics research
- Toxin Mechanisms and Immunotoxins
- Pharmacological Effects of Natural Compounds
- Biochemical and Molecular Research
Engineering and Physical Sciences Research Council
2016-2024
Biotechnology and Biological Sciences Research Council
2016-2024
University of Nottingham
2015-2024
NIHR Nottingham Digestive Diseases Biomedical Research Unit
2015
Wageningen University & Research
2013
Clostridium acetobutylicum represents a paradigm chassis for the industrial production of biofuel biobutanol and focus metabolic engineering. We have previously developed procedures creation in-frame, marker-less deletion mutants in pathogen difficile based on use pyrE codA genes as counter selection markers. In current study we sought to test their suitability C. acetobutylicum.Both systems readily allowed isolation in-frame deletions ATCC 824 spo0A cac824I genes, leading sporulation minus...
Biofuel production by Clostridium acetobutylicum is compromised strain degeneration due to loss of its pSOL1 megaplasmid. Here we used engineering biology stably integrate into the chromosome together with a synthetic isopropanol pathway. In membrane bioreactor continuously fed glucose mineral medium, final produced advanced biofuels, n-butanol and isopropanol, at high yield (0.31 g