A5063000680- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Clostridium difficile and Clostridium perfringens research
- Respiratory viral infections research
- Influenza Virus Research Studies
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Viral gastroenteritis research and epidemiology
- Pneumonia and Respiratory Infections
- vaccines and immunoinformatics approaches
- Microbial metabolism and enzyme function
- Cancer Research and Treatments
- Microbial bioremediation and biosurfactants
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- Metal Extraction and Bioleaching
- Toxin Mechanisms and Immunotoxins
- biodegradable polymer synthesis and properties
- Pharmacological Effects of Natural Compounds
- Bacteriophages and microbial interactions
- Fungal and yeast genetics research
- Chromium effects and bioremediation
- Biochemical and Molecular Research
- Microbial Fuel Cells and Bioremediation
- Infections and bacterial resistance
Biotechnology and Biological Sciences Research Council
2016-2024
Engineering and Physical Sciences Research Council
2016-2024
University of Nottingham
2009-2024
NIHR Nottingham Digestive Diseases Biomedical Research Unit
2015
Most bacteria can only be transformed with circular plasmids, so robust DNA integration methods for these rely upon selection of single-crossover clones followed by counter-selection double-crossover clones. To overcome the limited availability heterologous markers, here we explore novel strategies that do not employ them, and instead exploit (i) activation or inactivation genes leading to a selectable phenotype, (ii) asymmetrical regions homology control order recombination events. We focus...
Sophisticated genetic tools to modify essential biological processes at the molecular level are pivotal in elucidating pathogenesis of Clostridium difficile, a major cause healthcare associated disease. Here we have developed an efficient procedure for making precise alterations C. difficile genome by pyrE-based allelic exchange. The robustness and reliability method was demonstrated through creation in-frame deletions three genes (spo0A, cwp84, mtlD) non-epidemic strain 630Δerm two (spo0A...
// John T. Heap 1,5,* , Jan Theys 2,* Muhammad Ehsaan 1 Aleksandra M Kubiak Ludwig Dubois 2 Kim Paesmans Lieve Van Mellaert 3 Richard Knox 4 Sarah A. Kuehne Phillipe Lambin and Nigel P. Minton Clostridia Research Group, Centre for Biomolecular Sciences, School of Life The University Nottingham, Park, UK. Maastro Lab, Institute GROW, Maastricht, MD Netherlands. Molecular Bacteriology, Rega Medical Research, Leuven, Minderbroedersstraat,Belgium. Morvus Technology Limited, Ty Myddfai,...
Clostridium acetobutylicum represents a paradigm chassis for the industrial production of biofuel biobutanol and focus metabolic engineering. We have previously developed procedures creation in-frame, marker-less deletion mutants in pathogen difficile based on use pyrE codA genes as counter selection markers. In current study we sought to test their suitability C. acetobutylicum.Both systems readily allowed isolation in-frame deletions ATCC 824 spo0A cac824I genes, leading sporulation minus...
Abstract Background Syndromic surveillance often relies on patients presenting to healthcare. Community cohorts, although more challenging recruit, could provide additional population-wide insights, particularly with SARS-CoV-2 co-circulating other respiratory viruses. Methods We estimated the positivity and incidence of SARS-CoV-2, influenza A/B, RSV, trends in self-reported symptoms including influenza-like illness (ILI), over 2022/23 winter season a broadly representative UK community...
Biofuel production by Clostridium acetobutylicum is compromised strain degeneration due to loss of its pSOL1 megaplasmid. Here we used engineering biology stably integrate into the chromosome together with a synthetic isopropanol pathway. In membrane bioreactor continuously fed glucose mineral medium, final produced advanced biofuels, n-butanol and isopropanol, at high yield (0.31 g
This report provides tools for robust and predictable control of gene expression in the model lithoautotroph C. necator H16. To address a current need, we designed, built, tested promoters RBSs controlling answer question on how existing newly developed inducible systems compare, two positively (AraC/P araBAD - l -arabinose RhaRS/P rhaBAD -rhamnose) negatively (AcuR/P acuRI -acrylate CymR/P cmt -cumate) regulated were quantitatively evaluated their induction kinetics analyzed. establish if...
The hydrogen-utilizing strain Cupriavidus necator H16 (DSM 428) was sequenced using a combination of PacBio and Illumina sequencing. Annotation this reveals 6,543 protein-coding genes, 263 pseudogenes, 64 tRNA 15 rRNA genes.
Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built tested the pMTL70000 modular plasmids comprising standardised E. coli replicons, selectable markers application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy number compatibility.
Ethylene is a small hydrocarbon gas widely used in the chemical industry. Annual worldwide production currently exceeds 150 million tons, producing considerable amounts of CO2 contributing to climate change. The need for sustainable alternative therefore imperative. natively produced by several different microorganisms, including Pseudomonas syringae pv. phaseolicola via process catalyzed ethylene-forming enzyme (EFE), subsequent heterologous expression EFE has led ethylene non-native...
ObjectivesWe evaluated Nanopore sequencing for influenza surveillance.MethodsInfluenza A and B PCR-positive samples from hospital patients in Oxfordshire, UK, a UK-wide population survey winter 2022-23 underwent following targeted rt-PCR amplification.ResultsFrom 941 infections, successful was achieved 292/388(75%) available Oxfordshire samples: 231(79%) A/H3N2, 53(18%) A/H1N1, 8(3%) B/Victoria 53/113(47%) samples. Sequencing more at lower Ct values. Most same-sample replicate sequences had...
Abstract Background Syndromic surveillance often relies on patients presenting to healthcare. Community cohorts, although more challenging recruit, could provide additional population-wide insights, particularly with SARS-CoV-2 co-circulating other respiratory viruses. Methods We estimated positivity and incidence of SARS-CoV-2, influenza A/B, RSV, trends in self-reported symptoms including influenza-like illness (ILI), over the 2022/23 winter season a broadly representative UK community...
<title>Abstract</title> We engineered <italic>Clostridium acetobutylicum</italic> to continuously and stably produce advanced biofuels by integrating the pSOL1 megaplasmid into chromosome using an synthetic biology approach further <italic>sadh</italic> <italic>hydG</italic> from beijerinckii</italic> convert acetone isopropanol. In a membrane bioreactor, fed with glucose mineral medium, final strain produced at high yield (0.31 g·g<sup>-1</sup>), titre (15.4 g·L<sup>-1</sup>) productivities...
Abstract Objectives We evaluated Nanopore sequencing for influenza surveillance. Methods Influenza A and B PCR-positive samples from hospital patients in Oxfordshire, UK, a UK-wide population survey winter 2022-23 underwent following targeted rt-PCR amplification. Results From 941 infections, successful was achieved 292/388(75%) available Oxfordshire samples: 231(79%) A/H3N2, 53(18%) A/H1N1, 8(3%) B/Victoria 53/113(47%) samples. Sequencing more at lower Ct values. Most same-sample replicate...