The facultative chemolithoautotroph Cupriavidus necator H16 is able to grow aerobically either with organic substrates or H2 and CO2 s it can accumulate large amounts of (up 90%) poly (3-hydroxybutyrate), a polyhydroxyalkanoate (PHA) biopolymer. ability this organism co-utilize volatile fatty acids (VFAs) as sources carbon under mixotrophic growth conditions was investigated PHA production monitored. accumulation assessed aerobic conditions, individual VFAs in mixtures, three different...

Stable production of value-added products using a microbial chassis is pivotal for determining the industrial suitability engineered biocatalyst. Microbial cells often lose multicopy expression plasmids during long-term cultivations. Owing to advantages related titers, yields, and productivities when system compared with genomic integrations, plasmid stability essential industrially relevant biobased processes. Cupriavidus necator H16, facultative chemolithoautotrophic bacterium, has been...

Consolidated bioprocessing (CBP) is reliant on the simultaneous enzyme production, saccharification of biomass, and fermentation released sugars into valuable products such as butanol. Clostridial species that produce butanol are, however, unable to grow crystalline cellulose. In contrast, those saccharolytic predominantly ethanol, Clostridium thermocellum cellulolyticum, degrade cellulose with high efficiency due their possession a multienzyme complex termed cellulosome. This has led...

The thermophile Geobacillus thermoglucosidasius has considerable attraction as a chassis for the production of chemicals and fuels. It utilises wide range sugars oligosaccharides typical those derived from lignocellulose grows at elevated temperatures. latter improves rate feed conversion, reduces fermentation cooling costs minimises risks contamination. Full exploitation its potential been hindered by dearth effective gene tools. Here we designed tested collection vectors (pMTL60000 series)...

Abstract The healthy trophoblast does not express classical HLA-A and HLA-B products; therefore, an MHC-restricted recognition of trophoblast-presented Ags is unlikely. In the decidua also in peripheral blood pregnant women, γδ T cells significantly increase number. We investigated possible role Ags. PBL isolated from women as well those at risk for premature pregnancy termination were conjugated to choriocarcinoma (JAR) transfected with nonclassical HLA (HLA-E, HLA-G). To investigate...

Healthy multiparous Holstein-Friesian cows (n = 22, parity: 2-4) from a large-scale dairy herd in Hungary were subjected to an intravenous glucose tolerance test 10-15 days after calving. AluI genotype of growth hormone, several plasma metabolites and metabolic hormones determined, current previous lactation yields recorded. We also used the Revised Quantitative Insulin Sensitivity Check Index (RQUICKI) its modified version (RQUICKIBHB) for estimation peripheral insulin sensitivity. The...

The use of fossil fuels is no longer tenable. Not only are they a finite resource, their damaging the environment through pollution and global warming. Alternative, environmentally friendly, renewable sources chemicals required. To date, focus has been on using lignocellulose as feedstock for microbial fermentation. However, its recalcitrance to deconstruction making development economic processes extremely challenging. One solution generation an organism suitable in consolidated...

Abstract. The aim of this paper was to study the polymorphisms bovine growth hormone gene. authors genotyped 363 Hungarian Holstein-Friesian bull dams from 6 farms all over country. Two variants (L and V) gene digested with AluI enzyme were identified in experiment. Genotyping carried out by PCR-RFLP method. frequency data L V allele 0.93 0.07 respectively. Distribution three genotypes 87.05% (LL), 12.40% (LV) 0.55% (VV). studied population H-W equilibrium considering genotype distribution....

Living organisms can synthesize a wide range of macromolecules from small set natural building blocks, yet there is potential for even greater materials diversity by exploiting biochemical processes to convert unnatural feedstocks into new abiotic polymers. Ultimately, the synthesis these polymers in situ might aid coupling with synthetic matrices, and generation biohybrids or engineered living materials. The key step biohybrid preparation harness relevant biological pathways produce...

3-Hydroxypropionate (3-HP) is a versatile compound for chemical synthesis and potential building block biodegradable polymers. Cupriavidus necator H16, facultative chemolithoautotroph, an attractive production chassis has been extensively studied as model organism biopolymer production. Here, we engineered C. H16 3-HP biosynthesis from its central metabolism. Wild type can use carbon source, highly undesirable trait chassis. However, deletion of three (methyl-)malonate semialdehyde...

The hydrogen-utilizing strain Cupriavidus necator H16 (DSM 428) was sequenced using a combination of PacBio and Illumina sequencing. Annotation this reveals 6,543 protein-coding genes, 263 pseudogenes, 64 tRNA 15 rRNA genes.

Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built tested the pMTL70000 modular plasmids comprising standardised E. coli replicons, selectable markers application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy number compatibility.

Background: Neuromyelitis optica (NMO)–systemic lupus erythematosus (SLE) association is a rare condition characterized by multiple autoantibodies. Objective: To examine if, during the evolution of NMO, anti-AQP4 responses are part polyclonal B cell activation, and if T contribute. Methods: In 19 samples six patients who developed NMO SLE, we examined correlation AQP4-IgG1 IgM with (i) anti-MOG IgG IgM, (ii) anti-nuclear, anti-nucleosome anti-dsDNA antibodies, (iii) cytokines chemokines in...

Polyhydroxyalkanoates are attractive alternatives to traditional plastics. However, although polyhydroxybutyrate (PHB) is produced in large quantities by Cupriavidus necator H16, its properties far from ideal for the manufacture of plastic products. These may be improved through coproduction with 3-hydroxypropionate (3HP), which leads formation copolymer poly(3-hydroxybutyrate-co-3-hydroxypropionate) (poly(3HB-co-3HP). To achieve this, a pathway was designed enable C. H16 convert β-alanine...

Cupriavidus metallidurans CH34 exhibits extraordinary metabolic versatility, including chemolithoautotrophic growth; degradation of BTEX (benzene, toluene, ethylbenzene, xylene); high resistance to numerous metals; biomineralization gold, platinum, silver, and uranium; accumulation polyhydroxybutyrate (PHB). These qualities make it a valuable host for biotechnological applications such as bioremediation, bioprocessing, the generation bioelectricity in microbial fuel cells (MFCs). However,...

3-Hydroxypropionic acid (3-HP) is a promising platform chemical with various industrial applications. Several metabolic routes to produce 3-HP from organic substrates such as sugars or glycerol have been implemented in yeast, enterobacterial species and other microorganisms. In this study, the native metabolism of Cupriavidus necator was investigated manipulated it represents chassis for production fatty derivatives CO2 H2. When testing C. its tolerance towards 3-HP, noted that could utilise...

Abstract Bacterial pilin nanowires are protein complexes, suggested to possess electroactive capabilities forming part of the cells’ bioenergetic programming. Their role is thought be linked facilitating electron transfer between cells and external environment permit metabolism cell-to-cell communication. There a significant debate, with varying hypotheses as nature proteins currently lying type-IV pilin-based polymerised cytochrome-based filaments. Importantly, date, there very limited...

The objective of this study was to estimate the effect thyroglobulin (TG) locus on beef quality traits in some cattle breeds and investigate DGAT1 milk production Hungarian Holstein Friesian population. TG genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. At TT bulls showed highest fat percentage values longissimus dorsi muscle (m. dorsi); difference between CC significant. GC/GC cows had milk, protein yield values. Due...

Abstract. The objective of this study was to estimate the effect acylCoA-diacylglycerol-acyltransferase 1 (DGAT1), leptin and thyroglobulin (TG) loci on milk fat, protein yield in Holstein Friesian, Jersey Hungarian Simmental cows. Leptin DGAT1 genotypes were determined by qPCR assay, while TG identified using polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP). GC/GC cows had highest 305-day values. Difference between AA/AA significant (P<0.05). CC...