A5076897009- Microbial Metabolic Engineering and Bioproduction
- DNA and Biological Computing
- Probiotics and Fermented Foods
- DNA and Nucleic Acid Chemistry
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Microbial Natural Products and Biosynthesis
- Enzyme Production and Characterization
- Genomics and Phylogenetic Studies
- Biopolymer Synthesis and Applications
- Polyamine Metabolism and Applications
- Diet, Metabolism, and Disease
- RNA and protein synthesis mechanisms
- Microbial metabolism and enzyme function
- Diphtheria, Corynebacterium, and Tetanus
- Biofuel production and bioconversion
- Protein Interaction Studies and Fluorescence Analysis
- Advanced biosensing and bioanalysis techniques
- Analytical Chemistry and Chromatography
- Genomics and Chromatin Dynamics
- Molecular Junctions and Nanostructures
- Amino Acid Enzymes and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
Technical University of Munich
2024
Leipzig University
2023
University of Nottingham
2019-2020
Engineering and Physical Sciences Research Council
2019
Biotechnology and Biological Sciences Research Council
2019
Bielefeld University
2013-2016
Even tough DNA can be considered as a very stable long term storage medium, errors must expected during storage. From experiments it is evident that the most common error type due to are strand breaks. We address problem of correcting breaks in sequences resulting from composite synthesis. introduce novel channel model with realistic assumptions about Our proposed coding scheme employs marker codes correct single For this purpose, we generalize run-length-limited for setting and derive...
Abstract Background Arginine biosynthesis in Corynebacterium glutamicum consists of eight enzymatic steps, starting with acetylation glutamate, catalysed by N -acetylglutamate synthase (NAGS). There are different kinds known NAGSs, for example, “classical” ArgA, bifunctional ArgJ, ArgO, and S-NAGS. However, since C. possesses a monofunctional which catalyses only the fifth step arginine pathway, glutamate must be acetylated an as yet unknown NAGS gene. Results was investigated metabolome...
Adduct formation, fragmentation events and matrix effects impose special challenges to the identification quantitation of metabolites in LC-ESI-MS datasets. An important step compound is deconvolution mass signals. During this processing step, peaks representing adducts, fragments, isotopologues same analyte are allocated a distinct group, order separate from coeluting compounds. From these peak groups, neutral masses pseudo spectra derived used for metabolite via decomposition database...
3-Hydroxypropionic acid (3-HP) is a promising platform chemical with various industrial applications. Several metabolic routes to produce 3-HP from organic substrates such as sugars or glycerol have been implemented in yeast, enterobacterial species and other microorganisms. In this study, the native metabolism of Cupriavidus necator was investigated manipulated it represents chassis for production fatty derivatives CO2 H2. When testing C. its tolerance towards 3-HP, noted that could utilise...
The development and application of a self-sufficient whole-cell system for transaminase biotransformations is described. relies on an engineered strain <italic>Corynebacterium glutamicum</italic> that produces smart amine donors.
Composite DNA is a recent method to increase the base alphabet size in DNA-based data storage.This paper models synthesizing and sequencing of composite introduces coding techniques correct substitutions, losses entire strands, symbol deletion errors. Non-asymptotic upper bounds on codes with $t$ occurrences these error types are derived. Explicit constructions presented which can achieve bounds.
C. glutamicum is a bacterium used for biotechnological production of amino acids and other metabolites. Arginine commercial importance in cosmetic pharmaceutical industries as food additive. Here metabolomics proteomics data were acquired from three mutant strains compared to wildtype extracts gain insights into changes introduced by the rational strain design with aim increase arginine bacterial workhorse. Several known compounds biosynthetic pathway could automatically be identified using...
Abstract A cooperative catalytic process through palladium and Brønsted acid activation is developed for the conjugate addition of cyclic β-keto esters to azoalkenes directly followed by hemiaminal formation upon cyclization. This transformation enabled utilizing chiral Pd-aqua complexes as combined acid–base catalysts. Thus, bicyclic highly functionalized dihydropyrroles with two contiguous quaternary stereogenic centers are formed in excellent yields single diastereomers exceptional...
Abstract We investigate how to characterize the kinetic parameters of an aminotransaminase using a non-standard coupled (or auxiliary) enzyme assay, where peculiarity arises for two reasons. First, one products auxiliary is substrate primary and, second, we explicitly account reversibility reaction. Using singular perturbation theory, distinguished asymptotic limits in terms strength reverse reaction, which allows us determine deduce characterized enzyme. This establishes...