A5051531269- Protein Structure and Dynamics
- Chemical Synthesis and Analysis
- Migraine and Headache Studies
- Analytical Chemistry and Chromatography
- Epilepsy research and treatment
- Machine Learning in Bioinformatics
- Molecular spectroscopy and chirality
- Carbohydrate Chemistry and Synthesis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Alzheimer's disease research and treatments
- Education, Psychology, and Social Research
- Molecular Sensors and Ion Detection
- Enzyme Structure and Function
- Genetic and phenotypic traits in livestock
- RNA and protein synthesis mechanisms
- Hemoglobin structure and function
- Pharmacological Effects and Toxicity Studies
- Crystallography and molecular interactions
- Neurological Disorders and Treatments
- Mass Spectrometry Techniques and Applications
- X-ray Diffraction in Crystallography
- Click Chemistry and Applications
- Receptor Mechanisms and Signaling
- Crystallization and Solubility Studies
- Advanced Proteomics Techniques and Applications
Eötvös Loránd University
2015-2025
Research Network (United States)
2025
Hungarian Research Network
2025
Montavid Thermodynamic Research Group
2024
National Renewable Energy Laboratory
2023
University of Colorado Boulder
2023
Leibniz-Institute for New Materials
2023
Environmental Energy & Engineering
2023
The University of Adelaide
2023
Imperial College London
2023
A joint experimental-theoretical study of a bifunctional squaramide-amine-catalyzed Michael addition reaction between 1,3-dioxo nucleophiles and nitrostyrene has been undertaken to gain insight into the nature organocatalytic activation. For this highly stereoselective reaction, three previously proposed mechanistic scenarios for critical CC bond-formation step were examined. Accordingly, formation major stereoisomeric products is most plausible by one pathways that involve electrophile...
To evaluate efficacy and tolerability of adjunctive lacosamide in children adolescents with uncontrolled focal (partial-onset) seizures.In this double-blind trial (SP0969; NCT01921205), patients (age ≥4-<17 years) seizures were randomized (1:1) to lacosamide/placebo. After a 6-week titration, who reached the target dose range for their weight (<30 kg: 8-12 mg/kg/d oral solution; ≥30-<50 6-8 ≥50 300-400 mg/d tablets) entered 10-week maintenance period. The primary outcome was change seizure...
Miniproteins are adequate models to study various protein-structure modifying effects such as temperature, pH, point mutation(s), H-bonds, salt bridges, molecular packing, etc. Tc5b, a 20-residue Trp-cage protein is one of the smallest with stable 3D fold (Neidigh J. W. et al. (2002) Nat. Struct. Biol. 9, 425−430). However, Tc5b exhibits considerable heat-sensitivity and only at relatively low temperatures. Here we report systematic investigation structural factors influencing stability by...
We present the further development and fine-tuning of an efficient, economic (≤3 equivalents activated amino acid ), environmentally friendly (6 mL organic waste/cycle) procedure for peptide synthesis using a fast coupling reaction (1.7 min). The designed setup can assist highly pure (>80%) raw materials even long (up to 30 aa.) and/or difficult sequences. significant reduction time by effective PyAOP N,N′-diisopropylcarbodiimide/HOBt reagents was achieved, virtually racemization-free...
Abstract Trp‐cage miniprotein was used to investigate the role of a salt‐bridge (Asp 9 –Arg 16 ) in protein formation, by mutating residues at both sides, we mapped its contribution overall stability and folding mechanism. We found that above side‐chains are also part dense interaction network composed electrostatic, H‐bonding, hydrophobic, etc. components. To elucidate fold stabilizing effects, compared contrasted electronic circular dichroism NMR data miniproteins equipped with those...
The amyloid state of proteins is widely studied with relevance to neurology, biochemistry, and biotechnology. In contrast nearly amorphous aggregation, the has a well-defined structure, consisting parallel antiparallel β-sheets in periodically repeated formation. understanding growing development novel molecular imaging tools, like cryogenic electron microscopy. Sequence-based predictors were developed, mainly using artificial neural networks (ANNs) as underlying computational technique....
In the era of peptide therapeutics, solid phase synthesis is becoming increasingly important in pharmaceutical industry and related research. However, high cost large amount toxic waste generated during production overshadow current technology, requiring reduction excess reagents replacement solvents used. Advances have been made to replace N,N-dimethylformamide with moderate success. Here, we report a recyclable anisole/dimethyl sulfoxide based carefully tuned solvent system that compatible...
Polypeptides can self‐assemble into highly organized amyloid structures through complex and poorly understood mechanisms. To better understand the key parameters governing amyloidogenesis, we investigated aggregation of Sup35 prion‐derived GNNQQNY sequence alongside two rationally designed mutants, glutamine to norleucine in position 4th or 5th, where selective removal hydrogen bonding capacity reduces structural stability. Our findings reveal that β‐sheet arrays form rapidly as an initial...
Single alpha-helices (SAHs) are protein regions with unique mechanical properties, forming long stable monomeric helical structures in solution. To date, only a few naturally occurring SAH have been extensively characterized, primarily from myosins, leaving the structural and dynamic variability of largely unexplored. Drebrin (developmentally regulated brain protein) contains predicted segment sequence characteristics, including aromatic residues within region preference for arginine over...
Short amyloidogenic oligopeptides (APRs) are proposed as early macromolecules capable of forming solvent‐separated nanosystems under prebiotic conditions. This study provides experimental evidence that APRs, such the aggregation‐prone oligopeptide A (APR‐A), can undergo mutational transitions to form distinct variants, and convert APR‐B, either amyloid‐like or water‐soluble non‐aggregating. These occur along a spectrum from strongly (pro‐amyloid) weakly (anti‐amyloid), with mutation sequence...
Background.—The serotonin transporter gene is a promising candidate locus for the genetic susceptibility of migraine. Objective.—Two functional polymorphisms (5‐HTTLPR and STin2) were analyzed to assess whether these variants are associated with pediatric Methods.—Eighty‐seven Hungarian migraine patients 464 controls genotyped using polymerase chain reaction. Patients suffering from (n = 38) or without aura 49) interviewed regarding clinical symptoms before during attacks. Results.—There was...
The primary objective of the TEENZ Study (NCT01211145) was to assess efficacy zolmitriptan nasal spray in acute treatment adolescent migraine patients (ages 12 17 years), as measured by outcome variable pain-free status at 2 hours post-treatment.This randomized, double-blind, placebo-controlled, four-arm parallel group study compared with placebo a single episode migraine. Patients completed 30-day run-in period treat attack single-blind spray. Eligible patients, who had not responded...
Exendin-4 (Ex4) is a potent glucagon-like peptide-1 receptor agonist, drug regulating the plasma glucose level of patients suffering from type 2 diabetes. The molecule's poor solubility and its readiness to form aggregates increase likelihood unwanted side effects. Therefore, we designed Ex4 analogues with improved structural characteristics better water solubility. Rational design was started parent 20-amino acid, well-folded Trp cage (TC) miniprotein involved step-by-step N-terminal...
Gonadotropin releasing hormone-III (GnRH-III), a native isoform of the human GnRH isolated from sea lamprey, specifically binds to receptors on cancer cells enabling its application as targeting moieties for anticancer drugs. Recently, we reported identification novel daunorubicin–GnRH-III conjugate (GnRH-III–[ 4 Lys(Bu), 8 Lys(Dau=Aoa)] with efficient in vitro and vivo antitumor activity. To get deeper insight into mechanism action our lead compound, cellular uptake was followed by confocal...
Three chiral α-(nonafluoro-tert-butoxy)carboxylic acids (R)-1, (RS)-2, (R)-3 were synthesized to examine their application as solvating agents with amines. As a model compound, first (S)- and/or (RS)-α-phenylethylamine was used, and diastereomeric salts investigated by 1H 19F NMR ECD spectroscopy. The spectroscopic studies carried out at room temperature using the slightly polar CDCl3 apolar C6D6 solvents in 5 mM 54 concentrations. difference of chemical shifts (Δδ) complexes is comparable...
Neurofibromatosis type 1 is a tumor predisposition syndrome inherited in autosomal dominant manner. Besides the intragenic loss-of-function mutations NF1 gene, large deletions encompassing gene and its flanking regions are responsible for development of variable clinical phenotype. These titled as microdeletions lead to more severe phenotype than those observed patients with mutations. Around 5-10% cases harbor deletion four major types (type 1, 2, 3 atypical) have been identified so far....
The smart peptide chemistry in flow method, or SPF for short, has been fine-tuned to enable the efficient, rapid, cost-effective and more environmentally friendly synthesis of small domain proteins, each representing a basic fold type.