A5090871488- Postharvest Quality and Shelf Life Management
- Plant Gene Expression Analysis
- Plant Physiology and Cultivation Studies
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant biochemistry and biosynthesis
- Phytochemicals and Antioxidant Activities
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Essential Oils and Antimicrobial Activity
- Horticultural and Viticultural Research
- Plant nutrient uptake and metabolism
- Ethnobotanical and Medicinal Plants Studies
- Plant Genetic and Mutation Studies
- Polysaccharides and Plant Cell Walls
- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Flowering Plant Growth and Cultivation
- Plant Virus Research Studies
- Antioxidant Activity and Oxidative Stress
- Plant Pathogens and Fungal Diseases
- Phytochemistry and Biological Activities
- RNA modifications and cancer
- Plant-Microbe Interactions and Immunity
ZheJiang Academy of Agricultural Sciences
2013-2025
Zhejiang University
2016-2025
University of Nottingham
2015-2024
Huazhong Agricultural University
2024
Loughborough University
1984-2022
Planta
2018
University of Fort Hare
2002-2015
Research International (United States)
2005-2009
Ghent University
2009
Weizmann Institute of Science
2009
Abstract 1-Aminocyclopropane-1-carboxylic acid synthase (ACS) is one of the key regulatory enzymes involved in synthesis hormone ethylene and encoded by a multigene family containing at least eight members tomato (Lycopersicon esculentum). Increased production accompanies ripening tomato, this coincides with change regulation from auto-inhibitory to autostimulatory. The signaling pathways that operate bring about transition so-called system-1 system-2 are unknown, we have begun address these...
Loss of seed viability and decrease in germination rate appear to be due two independent sets events occurring during artificial ageing tomato (Lycopersicon esculentum Mill) seeds. Only the second category physiological damage is reversible: a low temperature pre-sowing treatment being capable greatly reducing time fifty percent both unaged aged seeds, but having no effect on capacity. Leakage ionic solutes from seeds did not increase following ageing, there were considerable increases...
The tomato ACC oxidase gene family is comprised of three members designated ACO1, ACO2 and ACO3 . These are highly homologous throughout the protein coding regions but do show a degree sequence divergence within 3′ untranslated regions. have been cloned used as gene‐specific probes to analyse differential expression in various tissues at different stages development. Results indicate that all genes transcriptionally active display high inducibility number organs life cycle. Both ACO1...
Abstract There are at least five lipoxygenases (TomloxA, TomloxB, TomloxC, TomloxD, and TomloxE) present in tomato (Lycopersicon esculentum Mill.) fruit, but their role generation of fruit flavor volatiles has been unclear. To assess the physiological TomloxC volatile C6 aldehyde alcohol compounds, we produced transgenic plants with greatly reduced using sense antisense constructs under control cauliflower mosaic virus 35S promoter. The expression level mRNA some was selectively by gene...
Ripening of the model fruit tomato (Solanum lycopersicum) is controlled by a transcription factor network including NAC (NAM, ATAF1/2, and CUC2) domain proteins such as No-ripening (NOR), SlNAC1, SlNAC4, but very little known about targets or how they regulate ripening. Here, we conducted systematic search fruit-expressed genes showed that silencing NOR-like1 (Solyc07g063420) using virus-induced gene (VIGS) inhibited specific aspects initiation was delayed 14 days when function inactivated...
Abstract The tomato non-ripening (nor) mutant generates a truncated 186-amino-acid protein (NOR186) and has been demonstrated previously to be gain-of-function mutant. Here, we provide more evidence support this view answer the open question of whether NAC-NOR gene is important in fruit ripening. Overexpression nor did not restore full ripening phenotype. Further analysis showed that NOR186 located nucleus binds but does activate promoters 1-aminocyclopropane-1-carboxylic acid synthase2...
RIPENING INHIBITOR (RIN)-deficient fruits generated by CRISPR/Cas9 initiated partial ripening at a similar time to wild-type (WT) but only 10% WT concentrations of carotenoids and ethylene (ET) were synthesized. RIN-deficient fruit never ripened completely, even when supplied with exogenous ET. The low amount endogenous ET that they did produce was sufficient enable initiation this could be suppressed the perception inhibitor 1-MCP. reduced production tomatoes due an inability induce...
Summary Flavanones and flavones are excellent source of bioactive compounds but the molecular basis their highly efficient production remains elusive. Chalcone isomerase (CHI) family proteins play essential roles in flavonoid biosynthesis little known about transcription factors controlling gene expression. Here, we identified a type IV CHI (designated as CitCHIL1) from citrus which enhances accumulation flavanones (CFLs). CitCHIL1 participates CFL biosynthetic metabolon assists cyclization...
Abscisic acid (ABA) is a dominant regulator of ripening and quality in non-climacteric fruits. Strawberry regarded as model fruit due to its extensive genetic studies proven suitability for transgenic approaches understanding gene function. research has contributed on color, flavor development, softening, recent years ABA been established core strawberry ripening, whereas ethylene plays this role climacteric Despite major difference, several components the interacting regulatory network...
Fruit softening, an irreversible process that occurs during fruit ripening, can lead to losses and waste postharvest transportation storage. Cell wall disassembly is the main factor leading loss of firmness, several ripening-associated cell genes have been targeted for genetic modification, particularly pectin modifiers. However, individual knockdown most wall-related has had minimal influence on integrity with notable exception pectate lyase. Compared disassembly, studies matrix,...
Tomato plants transformed with a copy of the fruit‐expressed phytoene synthase cDNA under control CaMV 35S promoter showed ectopic production carotenoids. High expressers were reduced in stature. The dwarf character was inherited an inverse relationship between expression and plant height. Severely affected also chlorophyll content young leaves. These dwarfs 30‐fold reduction levels gibberellin A 1 (GA ) growth partially restored by treatment exogenous GA 3 . Qualitative quantitative changes...
The ethylene-forming enzyme (EFE), which catalyzes the last step in biosynthesis of plant hormone ethylene, has never been purified and no molecular probes are available. Recently, a putative cDNA clone for tomato EFE (pTOM13) identified by inhibiting ethylene synthesis with an antisense gene expressed transgenic plants. A direct test its function made expression pTOM13 Saccharomyces cerevisiae. After cloning artefacts were discovered 5' region cDNA, corrected (pRC13) was created fusion end...