A5078764103- Plant Gene Expression Analysis
- Postharvest Quality and Shelf Life Management
- Plant biochemistry and biosynthesis
- Plant Physiology and Cultivation Studies
- Plant Stress Responses and Tolerance
- Phytochemicals and Antioxidant Activities
- Horticultural and Viticultural Research
- Plant Molecular Biology Research
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Antioxidant Activity and Oxidative Stress
- Plant Surface Properties and Treatments
- RNA modifications and cancer
- Polysaccharides and Plant Cell Walls
- Light effects on plants
- Cancer Mechanisms and Therapy
- Seismology and Earthquake Studies
- GABA and Rice Research
- Hepatocellular Carcinoma Treatment and Prognosis
- Plant Reproductive Biology
- Orthoptera Research and Taxonomy
- Liver Disease Diagnosis and Treatment
- Geoscience and Mining Technology
- Fermentation and Sensory Analysis
Zhejiang University
2016-2025
Anhui Agricultural University
2023-2025
ZheJiang Academy of Agricultural Sciences
2012-2023
Ludong University
2020
Southern California University for Professional Studies
2019
University of Southern California
2019
Guelph Research and Development Centre
2018
Army Medical University
2018
Daping Hospital
2018
Agriculture and Agri-Food Canada
2018
Kiwifruit (Actinidia deliciosa) is a climacteric fruit sensitive to low concentrations of ethylene. To investigate the transcriptional mechanisms underlying kiwifruit ethylene response, transcription factors encoding four EIN3-Like (EILs) and 14 Ethylene Response Factors (ERFs) were cloned from kiwifruit. Expression these was examined during development. The expression transcripts most AdERFs higher early development, with exception AdERF3, which increased maturity. Several apparently...
Abstract Background Chinese bayberry ( Myrica rubra Sieb. and Zucc.) is an important subtropical fruit crop ideal species for quality research due to the rapid substantial changes that occur during development ripening, including in color taste. However, at molecular level limited by a lack of sequence data. The present study was designed obtain transcript data examine gene expression developing based on RNA-Seq bioinformatic analysis, provide foundation understanding mechanisms controlling...
The unique flavor of Citrus fruit depends on complex combinations soluble sugars, organic acids, and volatile compounds. monoterpene E-geraniol is an important volatile, contributing to in sweet orange (Citrus sinensis Osbeck). Moreover, antifungal activity has also been observed. However, the terpene synthase (TPS) responsible for its synthesis not identified orange. Terpene 16 (CitTPS16) was shown catalyze vitro, transient overexpression CitTPS16 fruits leaves Newhall resulted accumulation...
This review is concerned with the mechanisms controlling fruit softening. Master genetic regulators switch on ripening programme and regulatory pathway branches downstream, separate controls for distinct quality attributes such as colour, flavour, texture, aroma. Ethylene plays a critical role hormone implicated in different facets of ripening, including texture change, acting through range transcriptional regulators, this signalling can be blocked using 1-methylcyclopropene. A battery at...
Summary Flavanones and flavones are excellent source of bioactive compounds but the molecular basis their highly efficient production remains elusive. Chalcone isomerase (CHI) family proteins play essential roles in flavonoid biosynthesis little known about transcription factors controlling gene expression. Here, we identified a type IV CHI (designated as CitCHIL1) from citrus which enhances accumulation flavanones (CFLs). CitCHIL1 participates CFL biosynthetic metabolon assists cyclization...
Summary Anthocyanins are visual cues for pollination and seed dispersal. Fruit containing anthocyanins also appeals to consumers due its appearance health benefits. In kiwifruit ( Actinidia spp.) studies have identified at least two MYB activators of anthocyanin, but their functions in fruit the mechanisms by which they act not fully understood. Here, transcriptome small RNA high‐throughput sequencing were used comprehensively identify contributors anthocyanin accumulation kiwifruit. Stable...
Chlorophyll degradation naturally occurs during plant senescence. However, in fruit such as citrus, it is a positive characteristic, degreening an important colour development contributing to quality. In the present work, Citrus sinensis Osbeck, cv. Newhall was used model for chlorophyll degradation. An ethylene response factor, CitERF13, isolated and its transcriptional changes were closely correlated with peel or ethylene. Dual-luciferase yeast one-hybrid assays, well motif mutation,...
Lignin biosynthesis and its transcriptional regulatory networks have been studied in model plants woody trees. However, lignification also occurs some fleshy fruit has rarely considered this way. Loquat ( Eriobotrya japonica ) is one such convenient tissue for exploring the transcription factors involved regulating flesh lignification. Firmness lignin content of 'Luoyangqing' loquat were fund to increase during low-temperature storage as a typical symptom chilling injury, while heat...
During postharvest ripening of kiwifruit [Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson var. cv. Bruno] at 20 °C, six lipoxygenase (LOX) genes exhibited different expression patterns. AdLox1 and AdLox5 were up-regulated during ripening, transcript accumulation was delayed by 1-methylcyclopropene (1-MCP), whereas AdLox2, AdLox3, AdLox4, AdLox6 down-regulated with ripening. Levels two volatiles arising from the LOX pathway, that is, n-hexanal (E)-2-hexenal, highest after harvest...
Summary Lignin biosynthesis is regulated by many transcription factors, such as those of the MYB and NAC families. However, roles AP 2/ ERF factors in lignin have been rarely investigated. Eighteen Ej genes were isolated from loquat fruit ( Eriobotrya japonica ), which undergoes postharvest lignification during low temperature storage. Among these, expression 2‐1 , a transcriptional repressor, was negatively correlated with lignification. The dual‐luciferase assay indicated that could...
Ripening, including softening, is a critical factor in determining the postharvest shelf-life of fruit and controlled by enzymes involved cell wall metabolism, starch degradation, hormone metabolism. Here, we used transcriptomics-based approach to identify transcriptional regulatory components associated with texture, ethylene, degradation ripening kiwifruit (Actinidia deliciosa). Twelve differentially expressed structural genes, seven four ethylene biosynthesis, one 14 transcription factors...
Kiwifruit (Actinidia spp.) is a climacteric fruit with high sensitivity to ethylene, influenced by multiple ethylene-responsive structural genes and transcription factors. However, the roles of other post-transcriptional regulators (e.g. miRNAs) necessary for ripening remain elusive. High-throughput sequencing sRNAome, degradome transcriptome methods were used identify further contributors control in kiwifruit (A. deliciosa cv 'Hayward'). Two NAM/ATAF/CUC domain factors (AdNAC6 AdNAC7), both...
The persimmon fruit is a particularly good model for studying response to hypoxia, in particular, the hypoxia-response ERF (HRE) genes. An anaerobic environment reduces astringency by converting soluble condensed tannins (SCTs) into an insoluble form. Although physiology of de-astringency has been widely studied, its molecular control poorly understood. Both CO2 and ethylene treatments efficiently removed from 'Mopan' fruit, as indicated decrease SCTs. Acetaldehyde, putative agent causing...
Low temperature conditioning (LTC) alleviates peach fruit chilling injury but the underlying molecular basis is poorly understood. Here, changes in transcriptome, ethylene production, flesh softening, internal browning and membrane lipids were compared maintained constant 0 °C LTC (pre-storage at 8 for 5 d before storage °C). resulted a higher rate of production more rapid softening as result expression biosynthetic genes series cell wall hydrolases. Reduced was observed LTC, with lower...
China is an important centre of diversity for Prunus persica. In the present study, 17 Chinese peach cultivars were evaluated phenolic content and antioxidant activity. Neochlorogenic acid (NCHA), chlorogenic (CHA), procyanidin B1 (B1), catechin (CAT), cyanidin-3-O-glucoside (C3G), quercetin-3-O-galactoside (Q3GAL), quercetin-3-O-glucoside (Q3GLU), quercetin-3-O-rutinoside (Q3R), kaempferol-3-O-rutinoside (K3R) identified quantified. CHA CAT predominant components in both peel pulp this...
Aroma is a vital characteristic that determines the quality and commercial value of citrus fruits, volatiles have been analyzed in different species. In sweet orange, Citrus sinensis, sesquiterpene (+)-valencene key volatile compound fruit peel. Valencene synthesis catalyzed by terpene synthase CsTPS1, but transcriptional mechanisms controlling its gene expression are unknown. Here, AP2/ERF (APETALA2/ethylene response factor) transcription factor, CitAP2.10, characterized as regulator...
The RAV (related to ABI3/viviparous 1) group of transcription factors (TFs) play multifaceted roles in plant development and stress responses. Here, we show that strawberry (Fragaria × ananassa) FaRAV1 positively regulates anthocyanin accumulation during fruit ripening via a hierarchy activation processes. Dual-luciferase assay screening all fruit-expressed AP2/ERFs showed had the highest transcriptional promoter FaMYB10, key activator biosynthesis. Yeast one-hybrid electrophoretic mobility...
Summary Chloroplast development and chlorophyll(Chl)metabolism in unripe tomato contribute to the growth quality of fruit, however these mechanisms are poorly understood. In this study, we initially investigated seven homeobox‐containing transcription factors ( TF s) with specific ripening‐associated expression patterns using virus‐induced gene silencing VIGS ) technology found that inhibiting one s, BEL 1‐ LIKE HOMEODOMAIN 11 (Sl 11), significantly increased Chl levels fruit. This enhanced...
Flesh lignification is a specific chilling response that causes deterioration in the quality of stored red-fleshed loquat fruit (Eribotrya japonica) and one aspect wider injury. APETALA2/ETHLENE RESPONSIVE FACTOR (AP2/ERF) transcription factors are important regulators plant low-temperature responses lignin biosynthesis. In this study, expression action 27 AP2/ERF genes from cultivar 'Luoyangqing' were investigated order to identify regulating low-temperature-induced lignification. EjERF27,...
Significance A tomato fruit ripening–specific transcription factor, SlLOB1 predominantly influences cell wall–related gene regulation and textural changes during maturation thus is distinct from broadly acting ripening factors described to date that influence many processes. As such, an intermediate regulator primarily influencing a physiological subdomain of the overall transition.
Changes in skin colour, as a visual cue for fruit ripeness, are important physiological markers many crops including tomato, banana and grape. In kiwifruit, the remains brown during ripening, but de-greening of pericarp occurs to reveal accumulated carotenoids anthocyanins gold- red-fleshed cultivars. this study, analysis inner outer Actinidia chinensis 'Hongyang' revealed faster chlorophyll degradation pericarp, compared with pericarp. Based on transcriptome analysis, two...
Gene families associated with the ethylene signal transduction pathway in ripening kiwifruit (Actinidia deliciosa [A. Chev.] C.F. Liang et A.R. Ferguson var. cv. Hayward) were isolated from a expressed sequence tag (EST) database, including five receptor genes, two CTR1-like and an EIN3-like gene AdEIL1. All differentially among various vine tissues, none was fruit specific. During development, levels of transcripts AdERS1a, AdETR3, genes decreased, whereas those AdERS1b AdETR2 peaked at 97...