A5036554844- Crystallization and Solubility Studies
- Hepatitis C virus research
- X-ray Diffraction in Crystallography
- Hepatitis B Virus Studies
- Monoclonal and Polyclonal Antibodies Research
- Salmonella and Campylobacter epidemiology
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Liver Disease Diagnosis and Treatment
- Paraoxonase enzyme and polymorphisms
- interferon and immune responses
- Bacteriophages and microbial interactions
- Lysosomal Storage Disorders Research
- Enzyme Production and Characterization
- Adsorption and biosorption for pollutant removal
- Infant Nutrition and Health
- Chemotherapy-induced cardiotoxicity and mitigation
- Synthesis and biological activity
- Cynara cardunculus studies
- Cytokine Signaling Pathways and Interactions
- Seaweed-derived Bioactive Compounds
- Saffron Plant Research Studies
- Plant Growth Enhancement Techniques
- Systemic Lupus Erythematosus Research
- Allergic Rhinitis and Sensitization
Quaid-i-Azam University
2023
University of the Punjab
2010-2021
University of Sargodha
2016
Creative Commons
2011
University of Calgary
1989
Side-effects associated with the cancer chemotherapy limit scope of chemotherapeutic drugs and no data was available about these side effects in Pakistan. Moreover starvation based differential has been proved to greatly reduce depending on time. The current study conducted survey common role them. included total 100 subjects multiple carcinomas. A comprehensive questionnaire inquiry, their basic information filled by interviewers as told patients. There were 48% patients breast 11% uterine...
The emerging resistance of pathogen against the currently available antimicrobial agents demands search new agents. use medicinal plants as natural substitute is paramount area research to overwhelm drug infectious Scientists have not made enough effort on evaluation safety plant yet.In present study activity Lawsonia inermis investigated clinical isolates seven bacteria including four Gram negative (Escherichia coli, Salmonella typhi, Klebsiella spp., Shigella sonnei) and three positive...
Outbreaks of Escherichia coli O157:H7 have been attributed to meat or products, particularly ground meat, and unpasteurized dairy products. However, the risk factors for sporadic cases not clearly delineated. Study data were collected by using a self-administered dietary historical questionnaire on all patients whose history included bloody diarrhea who attended one participating emergency departments. Designation as "case" "control" occurred after stool culture results known, eliminating...
Hepatitis C virus (HCV) is a causative agent of chronic liver disease, cirrhosis, and hepatocellular carcinoma. In Pakistan, more than 10 million people are living with HCV. Very little known about the genotype distribution in Punjab Province, largest province Pakistan. Pretreatment identification very important, as different HCV genotypes respond differently to interferon therapy.In this study we performed genotyping for 1537 HCV-infected patients from districts Sequencing partial NS5B...
Cladosporium cladosporioides was identified as a laccase producer when grown on malt extract media supplemented with 0.0 2% ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid). Extracellular purified to homogeneity from culture supernatant of by acetone precipitation, gel filtration chromatography and anion exchange chromatography, also characterized. Purified (specific activity 1785.71 U·mg−1) is monomeric protein molecular weight 50 kDa SDS-PAGE. showed maximum at pH 5 optimal...
Helicobacter pylorus is considered for chronic gastritis, gastric ulcers and adenocarcinoma its high infection rate observed in overcrowded lower socioeconomic groups developing countries. This study was designed to identify the role of drinking water transmission prevalence H. pylori (HP). Selective HP medium developed enrichment presumptive identification by urease, catalase species specific 16S rRNA tests. The virulence genes (vacA ‘s’ ‘m’ regions cagA) 90 out 225 positive samples were...
The use of Moringa oleifera as natural food preservative has been evaluated in the present study. In addition, for quality assurance, study also focused on shelf life product to authenticate identification plant by development DNA based marker. Among different extracts prepared from flower pods oleifera, methanol and aqueous extract exhibited high antibacterial antioxidant activity, respectively. phenolic contents (53.5 ± 0.169 mg GAE/g) flavonoid (10.9 0.094 QE/g) were recorded extract, Due...
Interferon alpha 2b (IFNα-2b) is an important cytokine and used for antiviral anticancer treatment. The low cost production of IFNα-2b with high biological activity necessary to provide the interferon therapy hepatitis patients in Pakistan. In present study, human (hIFNα-2b) gene from a healthy person was cloned overexpressed E. coli BL21(DE3). molecular weight expressed hIFNα-2b 19 kDa. over recombinant checked by ELISA using antibodies raised against commercially available hIFNα-2b....
Polymorphisms in the interferon λ (INF λ) genes on chromosome 19 have been associated with clearance of hepatitis C virus (HCV) induced by and ribavirin therapy however there is no such data available for Pakistani patients HCV infection.In this study, effects single nucleotide polymorphisms (SNPs) investigated response to treatment interferon-α a cohort 75 genotype 3a patients.A total number 50 SNPs from Interferon region were genotyped investigate allelic associations type patients....
Beta-mannosidase (EC 3.2.1.25, MANB) dissects the non-reducing end of N-linked mannose moieties glycoproteins in eukaryotic cells. The human beta-mannosidase gene was amplified by RT-PCR, cloned and sequenced. DNA sequence compared with reported sixteen nucleotide differences were found. deduced amino-acid showed that seven codons coded same amino acids nine different reference to substitution positions but did not affect recombinant MANB enzyme activity. No splice mutation observed after...
Resistance to commercially available antimicrobials is increasing at alarming rate therefore there an urgent need of new antimicrobial compounds and materials with novel modes action tackle the problem resistance in pathogenic gram positive negative bacteria. The current study involves design synthesis molecular structures which should not resemble basic existing classes agents order avoid risks cross development. A total 14 metal-barbiturate complexes (newly synthesized compounds) were...
Abstract Interferon therapy for the treatment of hepatitis C virus infection has very limited clinical application due to short serum half-life and side effects in systemic route administration. In present study, we have focused improve interferon by overcoming limitation effects. We hypothesized that latent alpha 2b (IFNα2b) produced fusion Latency associated protein (LAP) domain TGFβ IFNα2b having HCV NS3 protease cleavage site as linker will be activated only at target (liver) viral (HCV...
The present study is about the removal of lead and cadmium ions by biodegradable powdered biomass from an ornamental plant Gardenia jasminoides (GJB). characterization Fourier transformed infrared showed that consists amino, hydroxyl, carbonyl, halide functional groups. pHpzc concentration acidic sites (10.5 mmol/g) basic (7 were also determined. effects environmental parameters (varying initial Pb(II) Cd(II) concentrations, time contact, pH) studied. At contact times 20 30 min, sorption...
Human interferon α2 (IFNα2) and thymosin α1 (Tα1) are therapeutic proteins used for the treatment of viral infections different types cancer. Both IFNα2 Tα1 show a synergic effect in their activities when combination. Furthermore, fusion produced through genetic two genes can exhibit several functions one molecule. In this study, we determined anticancer antiviral human α2–thymosin protein (IFNα2–Tα1) our laboratory first time. The cytotoxic genotoxic IFNα2–Tα1 was evaluated HepG2 MDA-MB-231...