A5101604999- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Renal and related cancers
- Animal Genetics and Reproduction
- Nicotinic Acetylcholine Receptors Study
- Chromosomal and Genetic Variations
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- MicroRNA in disease regulation
- Memory and Neural Mechanisms
- Genomic variations and chromosomal abnormalities
- Zebrafish Biomedical Research Applications
- Angiogenesis and VEGF in Cancer
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Biomedical Ethics and Regulation
- Single-cell and spatial transcriptomics
- Liver Diseases and Immunity
- Congenital heart defects research
- Coronary Interventions and Diagnostics
- RNA Interference and Gene Delivery
- Signaling Pathways in Disease
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
Huazhong University of Science and Technology
2024
Wuhan Children's Hospital
2024
Qingdao University
2024
Affiliated Hospital of Qingdao University
2024
State Key Laboratory of Oncogene and Related Genes
2023
University of California, Los Angeles
2017-2022
Tsinghua University
2022
Broad Center
2017-2020
National Institute of Biological Sciences, Beijing
2010-2016
Beijing Normal University
2014-2015
In vitro gametogenesis is the process of making germline cells from human pluripotent stem cells. The foundation this model quality first progenitors called primordial germ (PGCs), which in vivo are specified during peri-implantation window development. Here, we show that PGC (hPGC) specification begins at day 12 post-fertilization. Using single-cell RNA sequencing hPGC-like (hPGCLCs) differentiated cells, discovered hPGCLC involves resetting pluripotency toward a transitional state with...
Germ cells are essential to pass DNA from one generation the next. In human reproduction, germ cell development begins with specification of primordial (PGCs) and a failure specify PGCs leads infertility. Recent studies have revealed that transcription factor network required for PGC has diverged in mammals, this significant impact on our understanding reproduction. Here, we reveal Hominidae-specific Transposable Elements (TEs) LTR5Hs, may serve as TEENhancers (TE Embedded eNhancers)...
The development of an in vitro system which human primordial germ cell-like cells (hPGCLCs) are generated from pluripotent stem (hPSCs) has been invaluable to further our understanding cell (hPGC) specification. However, the means evaluate next fundamental steps have not well established. In this study we describe a two dimensional extended culture that promotes proliferation specified hPGCLCs, without reversion state. We demonstrate hPGCLCs undergo partial epigenetic reprogramming,...
Background: Induced pluripotent stem cells (iPSCs) hold promise for a new era in treating heart failure. However, the functional microstructure of iPSC-derived cardiomyocytes (iPSC-CMs) and their ability to attach extracellular matrix recipient myocardium require further elucidation. Thus, we analyzed adhesion molecules iPSC-CM. Methods Results: Immunostaining analysis showed that iPSC-CMs were similar neonatal (CMs) expressing cytoskeletal proteins myosin heavy chain (MHC), light (MLC) 2a,...
The DNA dioxygenase Tet1 has recently been proposed to play an important role in the reprogramming of somatic cells pluripotency. Its oxidization product 5-hydroxymethylcytosine, formerly considered intermediate demethylation 5-methylcytosine, implicated as being epigenetic reprogramming. Here, we provide evidence that (T) can replace multiple transcription factors during cell and generate high-quality mouse induced pluripotent stem (iPSCs) with Oct4 (O). OT-iPSCs efficiently produce viable...
Differentiated somatic cells of various species can be reprogrammed into induced pluripotent stem (iPSCs) by ectopically expressing a combination several transcription factors that are highly enriched in embryonic (ESCs). The generation iPSCs large animals has raised the possibility producing genetically modified through nuclear transplantation approach. However, it remains unknown whether could used for generating cloned transfer method. Here, we show successful production viable mice from...
Transition from primed to naive pluripotency is associated with dynamic changes in transposable element (TE) expression and demethylation of imprinting control regions (ICRs). In mouse, ICR methylation TE are each regulated by TRIM28; however, the role TRIM28 humans less clear. Here, we show that a null mutation causes significant alterations both states human pluripotency, phenotypically this has limited effects on self-renewal, instead causing loss germline competency. Furthermore,...
Nucleosome organization determines the chromatin state, which in turn controls gene expression or silencing. remodeling occurs during somatic cell reprogramming, but it is still unclear to what degree re-established nucleosome of induced pluripotent stem cells (iPSCs) resembles embryonic (ESCs), and whether iPSCs inherit some residual from parental fibroblast cells. We generated genome-wide maps mouse ESCs reprogrammed belonging three different germ layers using a secondary reprogramming...
Calcineurin-NFAT signaling is critical for early lineage specification of mouse embryonic stem cells and embryos. However, its roles in somatic cell reprogramming remain unknown. Here, we report that calcineurin-NFAT has a dynamic activity plays diverse at different stages reprogramming. At the stage, transiently activated activation required successful late stage reprogramming, becomes barrier inactivation induction pluripotency. Mechanistically, contributes to through regulating multiple...
Copy number variations (CNVs) have been shown to be overrepresented in children with congenital heart disease (CHD). Genetic evaluation of CHD is currently underperformed China. We sought determine the occurrence CNVs CNV regions disease-causing potential among a large cohort Chinese pediatric patients and investigate whether these could important critical modifiers surgical intervention.CNVs screenings were performed 1,762 who underwent at least one cardiac surgery. status over 200 locus...
Differentiated somatic cells of various species could be reprogrammed into induced pluripotent stem (iPSCs) by ectopically expressing a combination several transcription factors which are highly enriched in embryonic (ESCs). The generation iPS large animals has raised the possibility producing genetically modified through nuclear transplantation approach. However, it remains unknown whether iPSCs used for generating cloned cell transfer (SCNT). Here, we show successful production viable mice...
<title>Abstract</title> Ulcerative colitis (UC) is a chronic inflammatory condition affecting the colon, with its global incidence on rise, yet effective treatments remain limited. Through screening using an NF-kB promoter dual fluorescence reporter system, we identified anti-hypertension drug lacidipine as capable of effectively inhibiting p65 and IκBα phosphorylation. In murine model dextran sulfate sodium (DSS)-induced colitis, demonstrated ability to mitigate colon lesions reduce...
Hematopoietic cells represent an attractive starting cell type for induced pluripotent stem (iPS) induction, yet the molecular mechanisms in hematopoietic reprogramming are poorly defined. In this study, we showed that long-term more amenable iPS induction among several and progenitor (HSPC) populations, is accompanied by earlier of transcriptional program involved promotion macromolecule metabolism proliferation. Notably, identified multiple signaling pathways exhibited distinct expression...
The germline is fundamental to reproduction. How cell precursors, called primordial germ cells (PGCs), establish and maintain identity during peri-implantation development in humans remains elusive. Here, we show using human embryo attachment culture that (hPGC) specification begins at day 12 post fertilization. Next, single RNA-sequencing of more than 100,000 differentiation into hPGC-like (hPGCLCs), hPGCLC vitro involves first resetting pluripotency towards a new transitional state with...