A5086394380- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Sirtuins and Resveratrol in Medicine
- Innovative Microfluidic and Catalytic Techniques Innovation
- Plant Stress Responses and Tolerance
- CRISPR and Genetic Engineering
- Fungal and yeast genetics research
- Genomics and Chromatin Dynamics
- Biochemical and Molecular Research
National Institutes of Health
2019-2022
National Institute of Dental and Craniofacial Research
2019-2022
University at Buffalo, State University of New York
2017
Eukaryotic initiation factor 5A (eIF5A)†,‡ is an essential protein that requires a unique amino acid, hypusine, for its activity. Hypusine formed exclusively in eIF5A post-translationally via two enzymes, deoxyhypusine synthase (DHPS) and hydroxylase. Each of the genes encoding these proteins, Eif5a, Dhps, Dohh, required mouse embryonic development. Variants EIF5A or DHPS were recently identified as genetic basis underlying certain rare neurodevelopmental disorders humans. To investigate...
Abstract A key unresolved issue in molecular evolution is how paralogs diverge after gene duplication. For multifunctional genes, duplication often followed by subfunctionalization. Subsequently, new or optimized properties may evolve once the protein no longer constrained to achieve multiple functions. potential example of this process yeast heterochromatin Sir3, which arose from conserved DNA replication Orc1. We previously found that Sir3 subfunctionalized In study, we investigated...
The polyamines putrescine, spermidine, and spermine are required for normal eukaryotic cellular functions. However, the minimum requirement varies widely, ranging from very high concentrations (mm) in mammalian cells to extremely low yeast Saccharomyces cerevisiae Yeast strains deficient polyamine biosynthesis (spe1Δ, lacking ornithine decarboxylase, spe2Δ, SAM decarboxylase) require externally supplied polyamines, but supplementation with as little 10-8 m spermidine restores their growth....
Abstract Eukaryotic initiation factor 5A (eIF5A) is an essential with a unique amino acid, hypusine, required for its activity. Hypusine formed exclusively in eIF5A by post-translational modification involving two enzymes, deoxyhypusine synthase (DHPS) and hydroxylase (DOHH). Each of the three genes, Eif5a, Dhps or Dohh mouse embryonic development. Variants EIF5A DHPS were recently identified as genetic basis underlying certain rare neurodevelopmental disorders humans. To investigate roles...