A5102928762- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Malaria Research and Control
- Mosquito-borne diseases and control
- Cell Image Analysis Techniques
- Renal Transplantation Outcomes and Treatments
- Viral Infections and Vectors
- Microbial infections and disease research
- Immunotherapy and Immune Responses
- Xenotransplantation and immune response
- Bacillus and Francisella bacterial research
- Finance, Taxation, and Governance
- Cytokine Signaling Pathways and Interactions
- Single-cell and spatial transcriptomics
- Gene expression and cancer classification
- Immunodeficiency and Autoimmune Disorders
- Structural Analysis of Composite Materials
- Sphingolipid Metabolism and Signaling
- Rabies epidemiology and control
- Comparative International Legal Studies
- Ubiquitin and proteasome pathways
- Diabetes and associated disorders
- Immune responses and vaccinations
Immune Tolerance Network
2012-2025
Benaroya Research Institute
2024
Naval Medical Research Command
2006-2008
GlaxoSmithKline (Belgium)
2008
Armed Forces Research Institute of Medical Science
2008
Walter Reed Army Institute of Research
2008
Rigel (United States)
2003-2004
Sunesis (United States)
2003
Abatacept is a CTLA-4-Ig fusion protein that binds to the costimulatory ligands CD80 and CD86 blocks their interaction with CD28 CTLA-4 receptors expressed by T cells, therefore inhibiting cell activation function. has shown clinical efficacy in treating some autoimmune diseases but failed show benefit other conditions. The reasons for these disparate results are not clear warrant further investigation of abatacept's mode action. Longitudinal specimens from Immune Tolerance Network's A...
The CD2-depleting drug alefacept (LFA3-Ig) preserved beta cell function in new-onset type 1 diabetes (T1D) patients. most promising biomarkers of response were late expansion exhausted CD8 T cells and rare baseline inflammatory islet-reactive CD4 cells, neither which can be used to measure responses the weeks after treatment. Thus, we investigated whether early changes immunophenotypes could serve as activity. We characterized by flow cytometry identified an exhausted-like population CD2low...
Plaque reduction neutralization tests (PRNTs) are commonly used for measuring levels of dengue virus (DENV) neutralizing antibodies. However, these assays lack a standardized format, generally have low sample throughput, and labor-intensive. The objective the present study was to evaluate two alternative DENV antibody assays: an enzyme-linked immunosorbent assay-based microneutralization (MN) assay, fluorescent cell sorter-based, DC-SIGN expresser dendritic (DC) assay. False-positive rates,...
The activation of T cells, mediated by the T-cell receptor (TCR), activates a battery specific membrane-associated, cytosolic and nuclear proteins. Identifying signaling proteins downstream TCR will help us to understand regulation immune responses contribute developing therapeutic agents that target regulation. In an effort identify novel molecules for we undertook large-scale dominant effector genetic screen using retroviral technology. We cloned characterized 33 distinct genes from over...
To identify novel components of the TCR signaling pathway, a large-scale retroviral-based functional screen was performed using CD69 expression as marker for T cell activation. In addition to known regulators, two truncated forms p21-activated kinase 2 (PAK2), PAK2DeltaL(1-224) and PAK2DeltaS(1-113), both lacking domain, were isolated in screen. The PAK2 truncation, PAK2DeltaL, blocked Ag receptor-induced NFAT activation TCR-mediated calcium flux Jurkat cells. However, it had minimal effect...
Effective quality assessment is an important part of any high-throughput flow cytometry data analysis pipeline, especially when considering the complex designs typical experiments applied in clinical trials. Technical issues like instrument variation, problematic antibody staining, or reagent lot changes can lead to biases extracted cell subpopulation statistics. These manifest themselves non-obvious ways that be difficult detect without leveraging information about study design other...
Identifying and phenotyping donor-reactive T cells in recipients following transplantation may further the understanding of interactions between recipient’s immune system donor antigen. Detecting is difficult as they are sparse number potentially hyporesponsive, especially operationally tolerant recipients. To this end, we have established optimized a novel flow-based assay to detect phenotype alloreactive cryogenically preserved PBMC samples. By resting healthy responder for one day...