Profound changes in the metabolism of cancer cells have been known for almost 100 years, and many aspects these continued to be actively studied discussed. Differences results various studies can explained by diversity tumours, which differing processes energy metabolism, limitations methods used. Here, using fluorescence lifetime needle optical biopsy a hepatocellular carcinoma (HCC) mouse model patients with HCC, we measured reduced nicotinamide adenine dinucleotide (NADH) phosphate...

Abstract This paper presents the results of experiments which were performed using optical biopsy system specially developed for in vivo tissue classification during percutaneous needle (PNB) liver. The proposed includes an probe small diameter acceptable use PNB feasibility studies and actual tests on laboratory mice with inoculated hepatocellular carcinoma clinical conditions patients liver tumors are presented discussed. Monte Carlo simulations carried out to assess diagnostic volume...

Abstract The impairments of cerebral blood flow microcirculation brought on by cardiac and respiratory arrest were assessed with multi‐modal diagnostic facilities, utilising laser speckle contrast imaging, fluorescence spectroscopy diffuse reflectance spectroscopy. results imaging show a notable reduction in small medium size vessels during few minutes arrest, while the same effect was observed large sinuses their branches circulatory cessation. Concurrently, redox ratio indicates...

This work presents results of in vivo and situ measurements hepatocellular carcinoma by a developed optical biopsy system. Here, we describe the technical details implementation fluorescence lifetime diffuse reflectance system, equipped with an original needle probe, compatible 17.5G standard. The observed setup were verified fresh solutions NADH FAD++, then applied murine model for characterisation inoculated (HCC) adjacent liver tissue. technique, supplemented blood oxygen saturation, made...

Maxillary sinus pathologies remain among the most common ENT diseases requiring timely diagnosis for successful treatment. Standard inspection approaches indicate low sensitivity in detecting maxillary pathologies. In this paper, we report on capabilities of digital diaphanoscopy combined with machine learning tools detection such We provide a comparative analysis two applied to diapahnoscopy data, namely, convolutional neural networks and linear discriminant analysis. The specificity values...

The paper describes the methodology and technical implementation of a multimodal approach for optical diagnostics in hepatopancreatobiliary organs focal diffuse neoplasms.Fine needle aspiration biopsy technique following cytological examination show its effectiveness safety but performing takes several days.However, problem real-time analysis pathological changes tissues remains relevant.The solution suggested is implementing methods (namely fluorescence spectroscopy reflectance...

Abstract Based on the combined use of polymerisable polyacrylamide, collagen and an aqueous solution Flavin adenine dinucleotide (FAD), we have developed a new technique for fabrication composite phantoms mimicking fluorescence properties human skin. A comparative analysis absorption scattering coefficients, refractive indices, as well spectra with different concentrations FAD measured CCD spectrometer hyperspectral camera is presented. To obtain values coefficients close to those skin, zinc...

Introduction . Breast cancer (BC) is the most common malignant tumor in women worldwide. It amounts more than 16 % of all cases neoplasms women. Modern advances radiological methods breast tumors detection significantly increased number non-palpable revealed during examinations, while further progress requires development improved minimally invasive techniques for obtaining biopsy material from using tools navigational controls. An approach with high potential direct evaluation local...

One of the widely used optical biopsy methods for monitoring cellular and tissue metabolism is time-resolved fluorescence. The use this method in liver has a high potential studying shift energy-type production from oxidative phosphorylation to glycolysis changes antioxidant defense malignant cells. On other hand, machine learning have proven be an excellent solution classification problems medical practice, including biomedical optics. We aim combine fluorescence measurements automate...

Currently, optical biopsy is a promising area of diagnosing the state tissues in real time during surgical treatment oncological diseases. The important part this direction development fluorescence imaging systems and ensuring accuracy calibration measurements. article describes system to define tumor resection margins abdominal organs. In study, we proposed low-cost tissue-mimicking phantom combining solid base liquid that suitable for quick depending on target endogenous fluorophores....

This study presents a hyperspectral optical imaging system for the diagnosis of infantile hemangiomas. Pilot studies were conducted in clinic on infants. As main results, parameters blood flow and saturation areas with hemangiomas calculated using previously developed neural network approach. The results indicate possibility this to monitor effectiveness therapy.

Modern LED light sources have many advantages for operational lighting. They make it possible to choose the optimal type of lighting surgeon during operations on certain organs and systems. The study was aimed at experimental selection parameters various in vivo normal pathological conditions using a controlled color-dynamic lamp. results showed that specific spectral are necessary types tissues; color balance adjusting improves contrast visualization individual tissues relative each other....

Abstract During surgery, it is important for the surgeon to determine optimal lighting conditions contrast imaging of operated objects. The paper analyzes various parameters a controlled colour-dynamic surgical lamp based on powerful led when contrasting individual organs and tissues model animals, as well optical themselves. results will allow developing library colours settings wide range operations.

The aim of the study was to compare metabolic activity brain cortex after acute hypoxia caused by impairment breathing or blood circulation. Male Wistar rats were randomized in two groups: impaired and circulation failure groups. Fluorescence under 365 450 nm excitation diffuse reflectance intensity at 550-820 range estimated. We found that long-term hypoxic conditions, notable changes occur. suppose oxygen deficiency causes an activation GABA shunt mechanism. In cases failure, fluorescence...

To determine optimal lighting conditions for contrast imaging of surgical objects, optical characteristics biological tissues and spectral smart light "LED light" source based on RGBW LED are compared. The organs have been investigated. Optimal during surgery were studied. colour working with individual against the background whole organism is selected. Perspective fixture application possibility dynamic control shown.

Abstract Liver cancer remains one of the most widespread types worldwide. The time spent on diagnosis is crucial factors ensuring effectiveness treatment. As percutaneous needle biopsy gold standard for liver diagnosis, it necessary to develop new methods improving this procedure. One promising directions multimodal optical biopsy. proposed protocol describes methodology real-time in vivo measurements lesions during liver. approach combines fluorescence and diffuse reflectance spectroscopy...

In this paper we demonstrate results of combined measurements by diffuse reflectance and fluorescence lifetime measuring techniques for real time liver cancer differentiation using a needle optical probe.

Presently, in the modern laser Doppler flowmetry (LDF) distribution of blood perfusion and its changes along shift frequencies are simply ignored and/or not properly addressed.Utilizing registered power spectrum photocurrent, we introduce an LDF signal processing approach suitable for expanding diagnostic capabilities technique.In particular, demonstrate that it is possible to determine how oscillations flow (cardiac, breathe, myogenic, etc.) distributed frequency.Wavelet analysis utilized...

The paper describes the results of experimental studies using custom developed optical biopsy system for diagnostics in vivo during procedure fine needle aspiration biopsy. Experimental were conducted laboratory mice with inoculated hepatocellular carcinoma. measurements carried out fluorescence spectroscopy and diffuse reflectance methods to reveal metabolic morphological changes tissues. show that approach is sensitive cancer detection. Quantified differences maximum spectra between tumor...

At the moment, percutaneous needle biopsy (PNB) remains gold standard for diagnosing liver cancer. However, relatively high probability of false-negative results can still be an issue with method. The introduction real-time feedback precise navigation tool is up-and-coming technology to immensely reduce mistakes in taking relevant tissue samples. This work presents technical details developed optical system, which implements fluorescence lifetime and diffuse reflectance measurements. Also,...