Biotemplating—a new concept for preparation of ceramic composite materials with biomorphic microstructures will be described. Biological carbon preforms (CB-templates) were derived from different wood structures by high-temperature pyrolysis at temperatures between 800 and 1800 °C used as templates infiltration gaseous or liquid Si to form SiC- SiSiC-ceramics. During processing, the microstructural details bioorganic retained. Cellular composites unidirectional porous morphologies...

Background: The present study was performed to determine whether there is a difference, with regard functional outcome and quality of life, between endoprosthetic replacement rotationplasty for the treatment malignant tumors distal part femur or proximal tibia.Methods: Sixty-seven patients, ages eleven twenty-four years at time diagnosis, had tumor tibia. A in thirty-three an done thirty-four patients. median duration follow-up six one month (range, two sixteen months). scale developed by...

BACKGROUND There is little information regarding an adequate surgical margin for local control of Ewing's sarcoma. METHODS Two hundred and forty-four patients (PTS) with sarcoma who were registered in the Cooperative Sarcoma Studies underwent treatment. Ninety-four PTS definitive surgery (surgery alone), 131 received postoperative irradiation, 19 preoperative irradiation. The margins distributed as follows: radical, 29 PTS; wide, 148 marginal, 39 intralesional, 28 PTS. impact on treatment...

The organic cation transporters 1 (OCT1) and 2 (OCT2) mediate drug uptake into hepatocytes renal proximal tubular cells, respectively. Multidrug toxin extrusion protein (MATE1) is a major component of subsequent export bile urine. However, the functional interaction OCTs MATE1 for transcellular transport oral antidiabetic metformin or 1-methyl-4-phenylpyridinium (MPP(+)) has not fully been characterized.Single-transfected Madin-Darby canine kidney (MDCK) cells as well double-transfected...

Horizontal gene transfer is a key step in the evolution of bacterial pathogens. Besides phages and plasmids, pathogenicity islands (PAIs) are subjected to horizontal transfer. The mechanisms PAIs within certain species or between different still not well understood. This study focused on High-Pathogenicity Island (HPI), which PAI widely spread among extraintestinal pathogenic Escherichia coli serves as model for general. We applied phylogenetic approach using multilocus sequence typing...

Abstract Background and Objectives One of the most difficult problems in tumor surgery is treatment pelvic tumors, particularly those periacetabular region. This retrospective study serves to analyze clinical functional outcome new surgical technique hip transposition. Patients Methods Sixty‐two patients were analyzed (chondrosarcoma 15, Ewing's sarcoma or PNET 26, osteosarcoma 14, synovial cell 2, others 5) clinical, oncological, evaluated. Results The procedure focused on transpositions...

Background The Cooperative Ewing Sarcoma Study (CESS 86), conducted by the German Society of Pediatric Oncology and Hematology (GPOH), was planned on basis results preceding CESS 81 study. prognostic significance tumor volume in localized sarcoma bone well documented trial. As a consequence, treatment intensity adapted to follow-up 86 trial: four-drug combination used amended for patients with large (≥ 100 ml), where ifosfamide substituted cyclophosphamide. Procedure From January 1986 June...

Twelve adult patients with pelvic sarcoma had implantation of a hemipelvic prosthesis. Eight resection, and four acetabulopubic resection. The implanted prosthesis was special Vitallium™ prosthesis, which specially designed for each patient the aid computer. At median followup 57 months, eight were free disease. In local relapse, two additional one hindquarter amputation, observed. three deep infection, removed; however, amputation. One dislocation hip loosening. Overall survival 70%,...

Limb salvage after resection of a pelvic sarcoma that involves the acetabulum represents surgical challenge. The ideal method reconstruction acetabular remains subject controversy, and outcome in terms impact therapy is still unknown. purpose this study was to determine surgery on health-related quality life function resection.Eighty-one patients with underwent at single institution. Functional evaluation quality-of-life examination were performed forty-five patients, these comprised group....

The aim of this study was to evaluate the regenerative potential cell-laden and cell-free collagen matrices in comparison microfracture treatment applied full-thickness chondral defects an ovine model.Animals (n = 30) were randomized into 5 groups, 7-mm full-cartilage-thickness set at trochlea medial condyle both knee joints treated as follows: 2 scaffolds (collagen I/III, Chondro-Gide(®); II, Chondrocell(®)) for covering microfractured (autologous matrix-induced chondrogenesis), colonized...

Abstract In this study, we tested the performance and biocompatibility of a composite β‐tricalcium phosphate (β‐TCP) to collagen as bone void filler (Cerasorb ® Ortho Foam) in rabbit distal femoral condyle model. β‐TCP is completely resorbable synthetic calcium addition matrix couples osteoconductive effects two components. Furthermore, malleable properties implant material during surgical applications for shape control will be enhanced. A critical size defect 6 mm diameter 10 depth was...

Abstract Carbon monoxide (CO) poisoning is one of the most common causes injury and death from poisoning. The primary objective therapy to eliminate CO patient as quickly possible prevent acute long-term effects. ideal treatment hyperbaric oxygen in a pressure chamber. However, chambers are scarce, therefore, normobaric (NBO), which, however, has limited efficacy. Here, we present full-scale batch system for extracorporeal hyperoxygenation (EHT), which facilitates elimination...

The carrier Endoplasmic Reticulum Adenylate Transporter1 (ER-ANT1) resides in the endoplasmic reticulum (ER) membrane and acts as an ATP/ADP antiporter. Mutant plants lacking ER-ANT1 exhibit a dwarf phenotype their seeds contain reduced protein lipid contents. In this study, we describe further surprising metabolic peculiarity of er-ant1 mutants. Interestingly, Gly levels leaves are immensely enhanced (26×) when compared with that wild-type plants. accumulation is caused by significantly...

Abstract Prosthetic lipoyl groups are required for the function of several essential multienzyme complexes, such as pyruvate dehydrogenase (PDH), α-ketoglutarate (KGDH), and glycine cleavage system (glycine decarboxylase [GDC]). How these proteins lipoylated has been extensively studied in prokaryotes yeast (Saccharomyces cerevisiae), but little is known plants. We earlier reported that mitochondrial fatty acid synthesis by ketoacyl-acyl carrier protein synthase not vital lipoylation...

We investigated the cytotoxic responsiveness of 40 cell lines derived from representatives Ewing's sarcoma family tumours (ESFT), i.e., (ES), peripheral primitive neuroectodermal tumour (pPNET) and Askin (AT), to necrosis factor–related apoptosis-inducing ligand (TRAIL). Incubation with TRAIL at 100 ng/ml induced death 24 hr in 19 26 ES, 11 12 pPNET 2 AT lines. Half-maximal concentrations (IC50 values) varied 0.1 20 ng/ml. displayed potent activity against freshly ESFT isolates. Cytotoxicity...

In the present in vivo study, regenerative potential of a new injectable bone substitute (IBS) composed beta-tricalcium phosphate (β-TCP) and hyaluronan was tested rabbit distal femoral condyle model. To achieve this, 2 defects 6 mm diameter 10 length were drilled into each femur total 12 animals. For animal, 1 hole filled with material, other left empty to serve as control. After 1, 3, months, process analyzed by radiography well histological histomorphometrical analysis. The results...

The incidence and characteristics of sacral infiltration in pelvic sarcomas were analyzed. Fifty-one patients with a sarcoma (chondrosarcoma, 15 patients; Ewing's sarcoma, 23 osteosarcoma, 13 patients) abutting the sacroiliac joint had surgical treatment. Tumor into sacrum was suspected based on preoperative images 18 histologic tumor invasion. There significant difference median volume without infiltration. One sarcomas, seven chondrosarcomas, osteosarcomas penetrated sacrum. Logistic...

We investigated the pharmacokinetics of piperacillin and tazobactam in extracellular space fluid inflamed soft tissues six patients with diabetic foot infection using vivo microdialysis found similar penetration for but not into noninflamed tissue.