Abstract An electrical stimulus is a new approach to neural differentiation of stem cells. In this work, the conjunctiva mesenchymal cells (CJMSCs) on 3D conductive fibrous scaffold silk fibroin (SF) and reduced graphene oxide (rGo) were examined. rGo (3.5% w/w) was dispersed in SF‐acid formic solution (10% w/v) nanofibrous fabricated using electrospinning method. SEM TEM microscopies used for characterization. CJMSCs cultured 2 impulse models (Current 1:115 V/m, 100‐Hz frequency current...

Adhesion bands are pathological fibrous tissues that create in the middle of and organs, often reasons intestinal obstruction, female infertility. Here, we explored anti-adhesive inflammatory capacities PEG/silk Ibuprofen-loaded PEG/Silk core-shell nanofibrous membranes, respectively. The ibuprofen-loaded Silk Fibroin-Poly ethylene Glycol (SF-PEG) membrane was fabricated by electrospinning considered terms morphology, surface wettability, drug release, degradation. To reveal capability for...

Abstract Tissue and stem cell encapsulation andtransplantation were considered as promising tools in the treatment of patients with diabetes mellitus. The aim this study was to evaluate effect microfluidic on differentiation trabecular meshwork mesenchymal cells (TM‐MSC), into insulin‐producing (IPCs) both vitro vivo. presence differentiated microfibers (three dimensional [3D]) tissue culture plates (TCPS; two [2D]) evaluated by detecting mRNA protein expression pancreatic islet‐specific...

Pituitary iron overload in patients with transfusion-dependent beta-thalassemia may lead to delayed puberty. Magnetic resonance imaging (MRI) has the potential estimate tissue concentration by detecting its paramagnetic effect and hypophyseal damage measuring dimensions indirectly.To investigate association of pituitary MRI findings pubertal status thalassemic as well demonstrate any priority appearance them.Twenty-seven beta-thalassemic patients, aged 15-25 years, were divided into 13...

Abstract The purpose of this study was to investigate miR‐7 overexpression effects on neural differentiation mesenchymal stem cells (MSCs) both two‐dimensional (2D) and three‐dimensional (3D) culture systems. We upregulated through lentiviral vector in trabecular meshwork MSCs (TMMSCs) polymers poly l ‐lactic acid/polycaprolactone fibrous scaffold were fabricated by electrospinning characterized using scanning electron microscopy (SEM) Fourier transform infrared (FTIR). Neural markers...

Introduction: Transplantation of stem cells with a nanofibrous scaffold is promising approach for spinal cord injury therapy. The aim this work was to differentiate neural-like from placenta-derived mesenchymal (PDMSCs) using suitable induction reagents in three (3D) and two dimensional (2D) culture systems. Methods: After isolation characterization PDMSCs, the were cultivated on poly-L-lactide acid (PLLA)/poly caprolactone (PCL) treated neuronal medium 7 days. Electron microscopy, qPCR,...

This study employs a comprehensive computational approach to identify and evaluate potential inhibitors of gamma D crystallin (CRYGD), key protein implicated in cataract formation. A library compounds, including natural products FDA-approved drugs, was screened using molecular docking simulations binding energy calculations. The top-scoring compounds were further assessed for drug-likeness properties toxicity. Molecular dynamics conducted on the most promising candidates investigate their...

This study aimed to fabricate the PCL-nanoparticles (NPs) loaded retinoic acid (RA) using microfluidic system for successful cellular uptake and induction of neuronal differentiation trabecular meshwork mesenchymal stem cells (TMMSCs).A used synthesize RA-loaded NPs, DLS, FTIR, TEM, UV-spectroscopy was recruited characterize release RA. Also, toxicity, uptake, differential TMMSCs have been assessed.According obtained results, spherical NPs (117.6±0.35 nm, ‒19.4±5.3) (121.6±0.75 ‒23.6±1.3)...

Cataracts are considered the underlying cause of blindness globally. When average age earth’s population is on rise, cataract-induced and visual dysfunction will increase dramatically. (1) Currently, only viable treatment for cataracts surgery, including removing opaque lens replacing it with an artificial one which has a huge impact improving patient’s quality life. Undoubtedly, have far-reaching repercussions countries in terms social economic burdens, example, increasing rate automobile...

Amyloid plaque, a protein aggregate typically associated with Alzheimer's disease, has also been observed in the brains of individuals autism spectrum disorder (ASD). This raised questions about potential connection between amyloid plaque and ASD development. While significance this finding is not fully understood, several theories propose mechanisms by which could contribute to development ASD. One theory suggests that amyloid-beta protein, main component disrupt normal brain function...

The opacification of the lens, called cataracts, is responsible for degrading visual acuity as a consequence scattering light it passes through lens. ocular lens plays pivotal part in focusing onto retina, and its clarity essential acuity. In 2020, prevalence cataracts had been 15.2 million cases among over 50 years globally. It has estimated by year 2050, approximately people united states will suffer from cataracts. According to statistics, increase with passing years.

Cataract diseases, characterized by the opacification of lens, pose a significant global health burden. Animal models are invaluable for understanding cataractogenesis and testing potential treatments [1]. The selenite-induced cataract model has been widely used due to its rapid onset predictable progression. However, this limitations that must be considered. This article reviews explores alternative approaches better represent complexity natural course human diseases [2].

Alzheimer's disease (AD) is a neurodegenerative disorder that affects millions of people worldwide, and its pathogenesis characterized by the formation amyloid plaque in brain. Current treatments for AD are limited only offer symptomatic relief, making development new therapies crucial area research. One promising approach use natural products, which have been used traditional medicine centuries rich source bioactive compounds. In this review, we discuss products treatment AD, with...

This article explores various regulatory and operational strategies aimed at accelerating drug development by shortening clinical trials. The length of traditional trials presents challenges in delivering timely therapies to patients, making it crucial identify approaches that expedite the process without compromising safety or data integrity. discussed include adaptive trial designs, utilizing surrogate endpoints, leveraging real-world evidence, flexibility, streamlined protocols...

Regulatory science is a vital component of pharmaceutical development and approval processes, ensuring the safety, efficacy, quality drugs. This article provides an overview background importance regulatory in industry. It highlights critical role plays supporting innovation creating predictable transparent environment. Recent advances are discussed, including use real-world evidence, innovative trial designs, advancements gene cell therapies. These developments have potential to...