A5070522261- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Silk-based biomaterials and applications
- Biochemical and Structural Characterization
- Adhesion, Friction, and Surface Interactions
- Polymer Surface Interaction Studies
- Microbial Metabolic Engineering and Bioproduction
- Peptidase Inhibition and Analysis
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Advanced Fluorescence Microscopy Techniques
- bioluminescence and chemiluminescence research
- Marine Biology and Environmental Chemistry
- Enzyme Catalysis and Immobilization
- Protein purification and stability
- Alzheimer's disease research and treatments
- Cephalopods and Marine Biology
- Calcium Carbonate Crystallization and Inhibition
- DNA and Nucleic Acid Chemistry
- Molecular Sensors and Ion Detection
Vellore Institute of Technology University
2024-2025
Nanyang Technological University
2016-2023
Material (Belgium)
2016
Yeungnam University
2011-2015
The underwater adhesive prowess of aquatic mussels has been largely attributed to the abundant post-translationally modified amino acid l-3,4-dihydroxyphenylalanine (Dopa) in mussel foot proteins (MFPs) that make up their threads. More recently, it suggested during thread fabrication, MFPs form intermediate fluidic phases such as liquid crystals or coacervates regulated by a liquid-liquid phase separation (LLPS) process. Here, is shown Dopa plays another central role fiber formation,...
There when the light goes out: A copper biosensor was created by introduction of metal-chelating noncanonical amino acid L-DOPA in place L-tyrosine into green fluorescent protein genetic-code engineering (see picture). The specific binding Cu2+ vitro modified reversible and resulted fluorescence quenching proportion to amount present. EDTA=ethylenediaminetetraacetic acid. Copper is an important transition-metal ion human body. It plays a crucial role as cofactor oxidative scavenging...
Suckerin proteins, recently discovered in the sucker ring teeth of squids, represent a family promising structural biomacromolecules that can form supramolecular networks stabilized by nanoconfined β-sheets. Exploiting this feature as well their specific amino acid composition, we demonstrate artificial suckerin-19 (S-19) be engineered into nanocarriers for efficient drug delivery and gene transfection vitro vivo. First, S-19 self-assembles β-sheet nanoparticles with controlled particle...
Abstract Nature has evolved several molecular strategies to ensure adhesion in aqueous environments, where artificial adhesives typically fail. One recently‐unveiled design for wet‐resistant is the cohesive cross‐β structure characteristic of amyloids, complementing well‐established surface‐binding strategy mussel adhesive proteins based on 3,4‐ l ‐dihydroxyphenylalanine (Dopa). Structural that self‐assemble into cross β‐sheet networks are suckerins discovered sucker ring teeth squids. Here,...
Biomineralization, the process by which mineralized tissues grow and harden via biogenic mineral deposition, is a relatively lengthy in many mineral-producing organisms, resulting challenges to study growth biomineralization of complex hard tissues. Arthropods are ideal model organisms because they regularly molt their exoskeletons new ones fast timescale, providing opportunities track mineralization entire Here, we monitored mantis shrimp dactyl club—a bioapatite-based structure with...
We describe the simple bioconjugation strategy in combination of periodate chemistry and unnatural amino acid incorporation. The residue specific incorporation 3,4-dihydroxy-l-phenylalanine can alter properties protein to conjugate into polymers. homogeneously modified will yield quinone residues that are covalently conjugated nucleophilic groups polysaccharide. This novel approach holds great promise for widespread use prepare conjugates synthetic biology applications.
Abstract Here, we have utilized the incorporation of non‐canonical amino acids as a tool kit to improve enzyme properties for organic synthesis applications. The global 3‐fluorotyrosine (FY) into ω‐transaminase (ω‐TA) give ω‐TA[FY] enhanced thermostability and solvent tolerance without altering substrate specificity enantioselectivity. Moreover, was able completely convert 25 mM acetophenone ( S )‐1‐phenylethylamine ee >99%) in presence 20% DMSO (v/v) which is ∼2‐fold higher when compared...
Abstract In general, conventional enzyme engineering utilizes 20 canonical amino acids to alter and improve the functional properties of proteins such as stability, activity. this study, we utilized noncanonical acid incorporation technique enhance ω‐transaminase (ω‐TA). Herein, enhanced stability ω‐TA by residue‐specific (4 R )‐fluoroproline [(4 )‐FP] successfully immobilized onto chitosan or polystyrene (PS) beads with site‐specifically incorporated L ‐3,4‐dihydroxyphenylalanine (DOPA)...
A novel thermostable ω-transaminase from <italic>Thermomicrobium roseum</italic> showing broad substrate specificity and high enantioselectivity was identified, expressed biochemically characterized it could produce chiral amines at temperature.
We explored the possibility of generating nonpoisonous, renewable, low cost and a completely biodegradable photosensitizer for dye-sensitized solar cells (DSSC) as an alternative to synthetic molecules that involve expensive, time-consuming tedious synthesis purification procedures. Several natural dyes from plants microbes had successfully been demonstrated photosensitizers develop biosensitized (BSSCs). The objective this work is next generation cleaner sensitizer BSSC using green...
Using a surface forces apparatus and an atomic force microscope, we characterized the adhesive properties of adsorbed layers two recombinant variants Perna viridis foot protein 5 (PVFP-5), main surface-binding in plaque Asian green mussel. In one variant, all tyrosine residues were modified into 3,4-dihydroxy-l-phenylalanine (DOPA) during expression using residue-specific incorporation strategy. DOPA is key molecular moiety underlying underwater mussel adhesion. other preserved. The layer...
One stone, two birds: Here, we have developed a simple and efficient method for the incorporation of multiple unnatural amino acids in single protein. This protein exhibited different novel functionalities acquired from genetically incorporated acids, which is an interesting not inherent property
Herein, we report on the precise design of a modular fusion protein amenable to construction nanocapsules by nanoprecipitation. The central squid suckerin-derived peptide block provides structural stability, whereas both termini from spider silk fibroins make highly soluble at physiological pH, critical requirement for nanoprecipitation process. With this design, consisting shells and oily cores with sizes in range 190–250 nm are built straightforward manner.
Wenn das Licht ausgeht: Ein Kupfer- Biosensor wurde durch gentechnischen Ersatz von L-Tyrosin in grün fluoreszierendem Protein die chelatisierende nichtkanonische Aminosäure L-DOPA hergestellt (siehe Bild). Die spezifische Bindung Cu2+ modifizierte war reversibel und führte zu einer mit dem Cu2+-Gehalt skalierenden Fluoreszenzlöschung. EDTA=Ethylendiamintetraessigsäure. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed,...
Lead is one of the most hazardous metals ubiquitous in environment, causing serious health hazards to organisms. Recently, fluorescent proteins such as GFP and Dsred were utilized for development reagent-less rapid metal sensors. Here, we demonstrate a lead-sensing that highly sensitive lead at micro molar concentrations.