A5052388217- Single-cell and spatial transcriptomics
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Bioinformatics and Genomic Networks
- Mental Health Treatment and Access
- Dental Radiography and Imaging
- Endodontics and Root Canal Treatments
- bioluminescence and chemiluminescence research
- Cancer Research and Treatments
- Health, Environment, Cognitive Aging
- Biotin and Related Studies
- Cell Image Analysis Techniques
- Substance Abuse Treatment and Outcomes
- Dental Anxiety and Anesthesia Techniques
- Advanced Fluorescence Microscopy Techniques
- Opioid Use Disorder Treatment
Broad Institute
2022-2024
The University of Melbourne
2016
Abstract Spatially charting molecular cell types at single-cell resolution across the 3D volume is critical for illustrating basis of brain anatomy and functions. Single-cell RNA sequencing has profiled in mouse 1,2 , but cannot capture their spatial organization. Here we used an situ method, STARmap PLUS 3,4 to profile 1,022 genes a voxel size 194 × 345 nm 3 mapping 1.09 million high-quality cells adult spinal cord. We developed computational pipelines segment, cluster annotate 230 by gene...
Abstract Spatially charting molecular cell types at single-cell resolution across the three-dimensional (3D) volume of brain is critical for illustrating basis anatomy and functions. Single-cell RNA sequencing (scRNA-seq) has profiled in mouse 1, 2 , but cannot capture their spatial organization. Here, we employed an situ technique, STARmap PLUS 3, 4 to map more than one million high-quality cells whole adult spinal cord, profiling 1,022 genes subcellular with a voxel size 194 X 345 nm 3D....
Abstract Several computational methods have recently been developed for characterizing molecular tissue regions in spatially resolved transcriptomics (SRT) data. However, each method fundamentally relies on smoothing transcriptomic features across neighboring cells. Here, we demonstrate that increases autocorrelation between cells, causing latent space to encode physical adjacency rather than spatial patterns. We find randomly sub-sampling neighbors before mitigates autocorrelation,...
Spatial omics technologies characterize tissue molecular properties with spatial information, but integrating and comparing data across different modalities is challenging. A comparative analysis tool that can search, match, visualize both similarities differences of features in space multiple samples lacking. To address this, we introduce CAST ( C ross-sample lignment S pa T ial omics), a deep graph neural network (GNN)-based method enabling spatial-to-spatial searching matching at the...
Background: Individuals with opioid use disorder (OUD) often have significant medical and behavioral health needs that are unaddressed. Opioid treatment programs (OTP) uniquely positioned to provide integrated services for OUD, physical mental but underutilized this purpose. This study aims describe the healthcare of OTP clients in order inform care implementation OTPs. Method: (n = 1261) an program Bronx borough New York City were assessed symptoms (e.g., anxiety, depression), chronic...
The aim of the present study was to develop a methodology for constant monitoring resistance fluid flow during functional loading and then compare efficacy 4-mm mineral trioxide aggregate (MTA) apical plugs full-length MTA root fillings.The canals 24 single-rooted teeth were instrumented assigned into two groups: group 1 (filled with plug gutta percha) 2 cementoenamel junction level). All subjected stepwise cyclic simulated interstitial pressure 25 cm H2 O applied apically. A Flodec machine...