A5017126755- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- DNA Repair Mechanisms
- Fungal and yeast genetics research
- Gene expression and cancer classification
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Genomics and Diagnostics
- Plant Pathogens and Fungal Diseases
- Oral microbiology and periodontitis research
- Plant-Microbe Interactions and Immunity
- Streptococcal Infections and Treatments
- CRISPR and Genetic Engineering
- Food Quality and Safety Studies
- Infective Endocarditis Diagnosis and Management
- Mycotoxins in Agriculture and Food
- Genomics and Chromatin Dynamics
- bioluminescence and chemiluminescence research
- Hormonal Regulation and Hypertension
- Cancer Research and Treatments
- Reproductive Biology and Fertility
- Ovarian function and disorders
Central South University
2025
Binzhou University
2024
Binzhou Medical University
2024
Massachusetts Institute of Technology
2021-2024
Northwest A&F University
2019-2024
National Institute of Biological Sciences, Beijing
2013-2024
Broad Institute
2021-2024
Hebei Medical University
2021-2024
Third Affiliated Hospital of Sun Yat-sen University
2023
Sun Yat-sen University
2023
Abstract Spatially charting molecular cell types at single-cell resolution across the 3D volume is critical for illustrating basis of brain anatomy and functions. Single-cell RNA sequencing has profiled in mouse 1,2 , but cannot capture their spatial organization. Here we used an situ method, STARmap PLUS 3,4 to profile 1,022 genes a voxel size 194 × 345 nm 3 mapping 1.09 million high-quality cells adult spinal cord. We developed computational pipelines segment, cluster annotate 230 by gene...
The precise control of messenger RNA (mRNA) translation is a crucial step in posttranscriptional gene regulation cellular physiology. However, it remains challenge to systematically study mRNA at the transcriptomic scale with spatial and single-cell resolution. Here, we report development ribosome-bound mapping (RIBOmap), highly multiplexed three-dimensional situ profiling method detect translatome. RIBOmap 981 genes HeLa cells revealed cell cycle-dependent translational colocalized...
A possible relationship between periodontitis and Alzheimer's disease (AD) has been reported. However, there is limited information on the association Porphyromonas gingivalis (P. gingivalis) periodontal infection pathological features of AD. The hypothesis that P. may cause cognitive impairment via age-dependent neuroinflammation was tested. Thirty 4-week-old (young) female C57BL/6 J mice were randomly divided into two groups, control group experimental group. 12-month-old (middle-aged)...
Abstract Quantifying RNAs in their spatial context is crucial to understanding gene expression and regulation complex tissues. In situ transcriptomic methods generate spatially resolved RNA profiles intact However, there a lack of unified computational framework for integrative analysis data. Here, we introduce an unsupervised annotation-free framework, termed ClusterMap, which incorporates the physical location identity RNAs, formulates task as point pattern problem, identifies biologically...
Abstract Spatiotemporal regulation of the cellular transcriptome is crucial for proper protein expression and function. However, intricate subcellular dynamics RNA remain obscured due to limitations existing transcriptomics methods. Here, we report TEMPOmap—a method that uncovers profiles across time space at single-cell level. TEMPOmap integrates pulse-chase metabolic labeling with highly multiplexed three-dimensional in situ sequencing simultaneously profile age location individual...
Biofilms account for a great deal of infectious diseases and contribute significantly to antimicrobial resistance. Efflux pumps confer resistance microorganisms involve multiple processes biofilm formation. pump inhibitors (EPIs) are attracting considerable attention as inhibition strategy. The regulatory functions efflux in formation such mediating adherence, quorum sensing (QS) systems, the expression biofilm-associated genes have been increasingly identified. versatile properties both...
Gene essentiality is a variable phenotypic trait, but to what extent and how essential genes can become dispensable for viability remain unclear. Here, we investigate 'bypass of (BOE)' - an underexplored type digenic genetic interaction that renders dispensable. Through analyzing on one the six chromosome arms fission yeast Schizosaccharomyces pombe, find that, remarkably, as many 27% them be converted non-essential by BOE interactions. Using this dataset identify three principles bypass:...
Mitogen-activated protein kinase (MAPK) cascades are activated by external stimuli and convert signals to cellular changes. Individual MAPKs have been characterized in a number of plant pathogenic fungi for their roles pathogenesis responses biotic or abiotic stresses. However, mutants deleted all the MAPK genes not reported filamentous fungi. To determine MAPK-less effects fungal pathogen, this study we generated three wheat scab fungus Fusarium graminearum. The Gpmk1 mgv1 Fghog1 triple had...
Fungal segmented negative-sense RNA viruses (SNSRVs) have been frequently found. Only the large segment encoding RNA-dependent polymerase (RdRp) has reported in most fungal SNSRVs, except for a few SNSRVs to encode nucleocapsids, nonstructural proteins, or movement proteins.
Anthracnose diseases caused by
ABSTRACT An increasing number of patients utilizing in vitro fertilization (IVF) and assisted reproductive technology (ART) are characterized as impaired or poor ovarian responders (PORs). Owing to its unclear molecular etiology, the management with age‐related characteristics remains a controversial complex clinical concern. Therefore, it is important identify understand etiological causes behind POR develop more effective efficient strategies for these patients. In this study, we report...
Histone acetylation, balanced by histone acetyltransferase (HAT) and deacetylase (HDAC) complexes, affects dynamic transitions of chromatin structure to regulate transcriptional accessibility. However, little is known about the interplay between HAT HDAC complexes in Fusarium graminearum , a causal agent Head Blight (FHB) that uniquely contains chromosomal regions enriched for house-keeping or infection-related genes. In this study, we identified ortholog human inhibitor growth (ING1) gene F...
Abstract Spatially charting molecular cell types at single-cell resolution across the three-dimensional (3D) volume of brain is critical for illustrating basis anatomy and functions. Single-cell RNA sequencing (scRNA-seq) has profiled in mouse 1, 2 , but cannot capture their spatial organization. Here, we employed an situ technique, STARmap PLUS 3, 4 to map more than one million high-quality cells whole adult spinal cord, profiling 1,022 genes subcellular with a voxel size 194 X 345 nm 3D....
Abstract Polyamines, such as putrescine, spermidine, and spermine, are crucial for plant defense against both abiotic biotic stresses. Putrescine is also known a significant inducer of deoxynivalenol (DON) production in Fusarium graminearum , the primary causal agent head blight (FHB). However, impact other polyamines on DON whether modifying polyamine biosynthesis could improve wheat resistance to FHB currently unknown. In this study, we demonstrate that key precursor components putrescine...
Ovarian cancer (OC) is one of the most lethal gynecologic malignancies and a leading cause death in world. Thus, this necessitates identification prognostic biomarkers which will be helpful its treatment.The gene expression profiles from The Cancer Genome Atlas (TCGA) GSE31245 were selected as training cohort validation cohort, respectively. Kaplan-Meier (KM) survival analysis was used to analyze difference overall (OS) between high low RB transcriptional corepressor 1 (RB1) groups. To...
Breast cancer (BRCA) is one of the most common cancers in women worldwide and a leading cause death from malignancy. This study was designed to identify novel biomarker for prognosticating survival BRCA patients.The prognostic potential eukaryotic translation initiation factor 4 gamma 1 (EIF4G1) assessed using RNA sequencing (RNA-seq) data The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) as training cohort validation set, respectively. functional enrichment analysis...
Circulating T follicular helper (cTfh) cells are known to be involved in numerous immune-mediated diseases, but their pathological role psoriasis is less fully investigated. Herein, we aimed identify whether cTfh contributed the pathogenesis of psoriasis. The frequency and function were compared between patients with vulgaris healthy controls, infiltration Tfh was detected lesional nonlesional skin tissues patients. Moreover, dynamic change before after acitretin treatment evaluated. Our...