A5010073387- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Ferroptosis and cancer prognosis
- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Fungal and yeast genetics research
- Single-cell and spatial transcriptomics
- Mycobacterium research and diagnosis
- HIV Research and Treatment
- Cellular transport and secretion
- Sperm and Testicular Function
- Cell Adhesion Molecules Research
- Hypothalamic control of reproductive hormones
- Yeasts and Rust Fungi Studies
- Herpesvirus Infections and Treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Microtubule and mitosis dynamics
- Reproductive Health and Technologies
- Bacteriophages and microbial interactions
- Fermentation and Sensory Analysis
- Genetic Neurodegenerative Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Natural Compounds in Disease Treatment
- Ubiquitin and proteasome pathways
Weill Cornell Medicine
2021-2024
MIND Research Institute
2021-2024
Cornell University
2021-2024
Center for Neuro-Oncology
2021-2024
National Institute of Biological Sciences, Beijing
2014-2023
Nankai University
2023
Guiyang Medical University
2023
Chinese Research Academy of Environmental Sciences
2023
Guangxi University
2019-2022
Binzhou University
2021
Abstract Single-nuclei RNA sequencing characterizes cell types at the gene level. However, compared to single-cell approaches, many single-nuclei cDNAs are purely intronic, lack barcodes and hinder study of isoforms. Here we present isoform (SnISOr-Seq). Using microfluidics, PCR-based artifact removal, target enrichment long-read sequencing, SnISOr-Seq increased barcoded, exon-spanning long reads 7.5-fold naive sequencing. We applied adult human frontal cortex found that exons associated...
Abstract RNA isoforms influence cell identity and function. However, a comprehensive brain isoform map was lacking. We analyze single-cell across regions, subtypes, developmental time points species. For 72% of genes, full-length expression varies along one or more axes. Splicing, transcription start polyadenylation sites vary strongly between types, protein architecture associate with disease-linked variation. Additionally, neurotransmitter transport synapse turnover genes harbor cell-type...
Spore killers in fungi are selfish genetic elements that distort Mendelian segregation their favor. It remains unclear how many species harbor them and diverse mechanisms are. Here, we discover two spore from a natural isolate of the fission yeast Schizosaccharomyces pombe. Both belong to previously uncharacterized wtf gene family with 25 members reference genome. These act strain-background-independent genome-location-independent manners perturb maturation spores not inheriting them. Spores...
Substance use disorders (SUD) and drug addiction are major threats to public health, impacting not only the millions of individuals struggling with SUD, but also surrounding families communities. One seminal challenges in treating studying human populations is high prevalence co-morbid conditions, including an increased risk contracting a immunodeficiency virus (HIV) infection. Of ~15 million people who inject drugs globally, 17% persons HIV. Conversely, HIV factor for SUD because chronic...
Gene essentiality is a variable phenotypic trait, but to what extent and how essential genes can become dispensable for viability remain unclear. Here, we investigate 'bypass of (BOE)' - an underexplored type digenic genetic interaction that renders dispensable. Through analyzing on one the six chromosome arms fission yeast Schizosaccharomyces pombe, find that, remarkably, as many 27% them be converted non-essential by BOE interactions. Using this dataset identify three principles bypass:...
Significance Statement The receptor discoidin domain 1 (DDR1) is activated by collagen, upregulated in injured kidneys, and contributes to kidney fibrosis, but how DDR1 controls fibrosis poorly understood. authors show that upon collagen stimulation, translocates the nucleus. To do this, must bind with SEC61B, a component of Sec61 translocon, as well nonmuscle myosin IIA β -actin. In nucleus, binds chromatin increase transcription IV, major fibrosis. study reveals novel mechanism whereby...
The performance and parameters of Li-ion battery are greatly affected by temperature. As a significant parameter, state charge (SOC) is temperature during the estimation process. In this paper, an improved equivalent circuit model (IECM) considering influence ambient temperatures surface (BST) on based second-order RC have been proposed. exponential function fitting (EFF) method was used to identify at 5 including −10°C, 0°C, 10°C, 25°C 40°C, relationship between internal resistance BST....
The transmembrane glycoprotein (gp41) of human immunodeficiency virus type-1 (HIV-1) has a long cytoplasmic domain unknown functional significance. To investigate the role carboxy-terminal (C-terminal) portion HIV-1 envelope protein in viral replication, infectivity, and cytopathogenicity, we examined properties panel mutants with variable deletions 3′-env region. Deletion C-terminal 76 amino acids did not abolish production reverse transcriptase upon transfection COS-1 cells. 6-14 appeared...
Centrosomes play critical roles in the cell division cycle and ciliogenesis. Sfi1 is a centrin-binding protein conserved from yeast to humans. Budding essential for initiation of spindle pole body (SPB; centrosome) duplication. However, recruitment partitioning centrosomal structures have never been fully investigated any organism, presumed importance tryptophans internal repeats remains untested. Here we report that fission yeast, instead doubling abruptly at SPB duplication remaining...
Although the fission yeast Schizosaccharomyces pombe is a well-established model organism, studies of natural trait variations in this species remain limited. To assess feasibility segregant-pool-based mapping phenotype-causing genes strains yeast, we investigated cause maltose utilization defect (Mal - ) S. strain CBS5557 (originally known as malidevorans ). Analyzing genome sequence revealed 955 nonconservative missense substitutions, and 61 potential loss-of-function variants including 47...
RNA isoforms influence cell identity and function. Until recently, technological limitations prevented a genome-wide appraisal of isoform on in various parts the brain. Using enhanced long-read single-cell sequencing, we comprehensively analyze multiple mouse brain regions, subtypes, developmental timepoints from postnatal day 14 (P14) to adult (P56). For 75% genes, full-length expression varies along one or more axes phenotypic origin, underscoring pervasiveness regulation across scales. As...
Abstract Multimodal measurements have become widespread in genomics, however measuring open chromatin accessibility and splicing simultaneously frozen brain tissues remains unconquered. Hence, we devised Single-Cell-ISOform-RNA sequencing coupled with the Assay-for-Transposase-Accessible-Chromatin (ScISOr-ATAC). We utilized ScISOr-ATAC to assess whether alterations convergently affect same cell types or divergently different ones. applied three major conditions: comparing (i) Rhesus macaque...
AbstractIntegrin α1β1 negatively regulates the generation of profibrotic reactive oxygen species (ROS) by inhibiting epidermal growth factor receptor (EGFR) activation; however, mechanism which it does this is unknown. In study, we show that caveolin-1 (Cav-1), a scaffolding protein binds integrins and controls signaling, participates in integrin α1β1-mediated EGFR activation. Integrin α1-null mesangial cells (MCs) have reduced Cav-1 levels, reexpression α1 subunit increases decreases...
Killer meiotic drivers (KMDs) skew allele transmission in their favor by killing progeny not inheriting the driver allele. Despite widespread presence eukaryotes, molecular mechanisms behind selfish behavior are poorly understood. In several fission yeast species, single-gene KMDs belonging to wtf gene family exert expressing a toxin and an antidote through alternative transcription initiation. Here we investigate how products of wtf-family KMD can act antagonistically. Both...
Abstract Nematodes such as Caenorhabditis elegans are powerful systems to study basically all aspects of biology. Their species richness together with tremendous genetic knowledge from C. facilitate the evolutionary biological functions using reverse genetics. However, ability identify orthologs candidate genes in other can be hampered by erroneous gene annotations. To improve annotation nematode model organism Pristionchus pacificus , we performed a genome-wide screen for potentially...
Abstract Background The discovery of the superbug mcr-1 -positive Escherichia coli (MCRPEC) has drew greet attention. Swine-origin multi-drug resistant MCRPEC been a potential threat to public health and safety. However, there were few detailed studies have reported on swine in Guangxi, South China. Results In this study, thirty-three strains detected from 142 E. 116 samples Guangxi 2018. Which could be classified into eight unique STs total six incompatibility plasmid groups (IncFI, IncHI1,...
RF amide-related peptide 3 (RFRP-3), the mammalian ortholog of gonadotropin-inhibitory hormone (GnIH), is a reproductive inhibitor and an endogenous orexigenic neuropeptide that may be involved in energy homeostasis. In this study, we evaluated effect acute or chronic RFRP-3 treatment (administered via intraperitoneal injection) on food intake, meal microstructure weight rats, as well mechanism through which glucose metabolism pancreas disposal tissues rat vivo. Our results showed...
Teratozoospermia is a rare disease associated with male infertility. Several recurrent genetic mutations have been reported to be abnormal sperm morphology, but the basis of tapered-head not well understood. In this study, whole-exome sequencing (WES) identified homozygous WD repeat domain 12 ( WDR12 ; p.Ser162Ala/c.484T>G) variant in an infertile patient spermatozoa from consanguineous Chinese family. Bioinformatic analysis predicted mutation pathogenic variant. To verify effect variant,...
Gonadotropin-inhibitory hormone (GnIH) is a reproductive inhibitor and an endogenous orexigenic neuropeptide that may be involved in energy homeostasis reproduction. However, whether GnIH molecular signal link of metabolism the system, thus, regulates activity as function state, still unknown. In present study, we investigated involvement glycolipid reproduction vivo, coupling between these two processes testis level. Our results showed chronic intraperitoneal injection into male mice not...