Background MicroRNAs (miRNAs) are the class of small endogenous RNAs that play an important regulatory role in cells by negatively affecting gene expression at transcriptional and post-transcriptional levels. There have been extensive studies aiming to discover miRNAs analyze their functions from a variety species. However, there no published miRNA profiles human testis using next generation sequencing (NGS) technology. Results We employed Solexa technology profile normal testis. Total 770...

Primordial follicles, providing all the oocytes available to a female throughout her reproductive life, assemble in perinatal ovaries with individual surrounded by granulosa cells. In mammals including mouse, most die apoptosis during primordial follicle assembly, but factors that regulate oocyte death remain largely unknown. Proliferating cell nuclear antigen (PCNA), key regulator many essential cellular processes, was shown be differentially expressed these processes mouse using 2D-PAGE...

In mammals, the primordial follicle pool, providing all oocytes available to a female throughout her reproductive life, is established perinatally. Dysregulation of assembly results in diseases, such as premature ovarian insufficiency and infertility. Female mice lacking Dicer1 (Dicer), gene required for biogenesis microRNAs, show abnormal morphology follicles However, contribution individual microRNAs remains largely unknown. Here, we report that microRNA 376a (miR-376a) regulates by...

Lumpy skin disease (LSD) is an infectious affecting large ruminants, caused by the lumpy virus (LSDV). It characterized symptoms such as hyperthermia, emaciation, lymphadenopathy, and nodules. Pregnant animals affected LSD often experience abortions may develop infertility syndrome, while males can become sterile. A live attenuated goat pox vaccine (GTPV) was administered to various groups of crossbred buffaloes. After vaccination, blood samples were collected from each group analyze...

Abstract Several different mutations in the proteome of centriole 1 centriolar protein B (POC1B) have been linked to cone dystrophy or cone-rod (CORD). However, POC1B that are associated with both CORD and oligoasthenoteratozoospermia (OAT) not reported previously. Here, whole-exome sequencing was performed identify a homozygous frameshift variant (c.151delG) two brothers who had diagnosed OAT from consanguineous family. Transcript analyses biological samples patients carrying showed is lost...

Inflammatory bowel diseases (IBDs), including ulcerative colitis (UC) and Crohn's disease (CD), are chronic inflammatory disorders caused by genetic influences, the immune system environmental factors. However, underlying pathogenesis of IBDs pivotal molecular interactions remain to be fully elucidated. The aim present study was identify signatures in patients with elucidate potential mechanisms IBD subtypes. gene expression profiles GSE75214 datasets were obtained from Gene Expression...

Kinesin-12 (also named Kif15) participates in important events during neuronal development, such as cell division of precursors, migration young neurons and establishment axons dendritic arbors, by regulating microtubule organization. Little is known about the molecular mechanisms behind functions kinesin-12, even less its roles other types nervous system. Here, we show that kinesin-12 depletion from cultured rat cortical astrocytes decreases proliferation but increases migration....

To explore that it is necessary to routinely detect chromosomes in infertile patients, we investigated peripheral blood lymphocyte karyotype 16,294 male patients the north-east of China and analysed incidence type chromosomal anomaly polymorphism. G-banding analysis lymphocytes was performed cases. Semen three times all men. PCR FISH confirmed presence SRY gene. The rate 4.15% (677/16,294). rates were 0.24% normal semen group, 12.6% light oligoasthenospermia 4.7% moderate-to-severe group...

Macrozoospermia is a rare sperm morphologic abnormality associated with male infertility and characterized by high percentage of spermatozoa large irregular heads. The aim this study was to identify the genetic cause an infertile macrozoospermia from consanguineous family. Whole-exome sequencing (WES) performed using peripheral blood genomic DNA patient his parents. WES analysis family allowed identification novel homozygous missense variant in AURKC gene (c.269G>A). Bioinformatics also...

Loss of function tuberous sclerosis complex 1 or 2 (TSC1 TSC2) leads to the activation mammalian target rapamycin (mTORC1). Hyperactivated mTORC1 plays a critical role in tumor growth, but underlying mechanism is still not completely elucidated. Here, by analyzing Tsc1- Tsc2-null mouse embryonic fibroblasts, rat ELT3 cells, and human cancer we present evidence for involvement epidermal growth factor receptor (EGFR) as downstream growth. We show that increased EGFR expression through...

Abstract The heavy metal cadmium is proposed to be one of the environmental endocrine disruptors spermatogenesis. Cadmium-induced inhibition spermatogenesis associated with a hormone secretion disorder. Letrozole an aromatase inhibitor that increases peripheral androgen levels and stimulates However, potential protective effects letrozole on cadmium-induced reproductive toxicity remain elucidated. In this study, male mice were administered CdCl 2 (4 mg/kg BW) orally by gavage alone or in...

Teratozoospermia is a rare disease associated with male infertility. Several recurrent genetic mutations have been reported to be abnormal sperm morphology, but the basis of tapered-head not well understood. In this study, whole-exome sequencing (WES) identified homozygous WD repeat domain 12 ( WDR12 ; p.Ser162Ala/c.484T>G) variant in an infertile patient spermatozoa from consanguineous Chinese family. Bioinformatic analysis predicted mutation pathogenic variant. To verify effect variant,...

Folliculogenesis is an important part of ovarian function as it provides the oocytes for female reproductive life. Characterizing genes/proteins involved in folliculogenesis fundamental understanding mechanisms associated with this biological and to cure diseases folliculogenesis. A large number have been identified from different species. However, no dedicated public resource currently available folliculogenesis-related that are validated by experiments. Here, we reporting a database...

Glial scar formation is a major obstacle to regeneration after spinal cord injury. Moreover, it has been shown that the astrocytic response injury differs between species. Gekko japonicas type of reptile and shows differential glial activation compared rats. The purpose present study was compare proliferation migration astrocytes in cords geckos rats vitro. Spinal homogenate stimulation scratch wound models were used induce adult embryonic rats, as well geckos. Our results indicated from rat...

Nonobstructive azoospermia (NOA) is a common cause of infertility and defined as the complete absence sperm in ejaculation due to defective spermatogenesis. The aim this study was identify genetic etiology NOA an infertile male from Chinese consanguineous family. A homozygous missense variant membrane-bound O-acyltransferase domain-containing 1 (MBOAT1) gene (c.770C>T, p.Thr257Met) found by whole-exome sequencing (WES). Bioinformatic analysis also showed that pathogenic amino acid residue...

Abstract Background Acute graft-versus-host disease (aGVHD) is a life-threatening complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT). Accumulating evidence suggests that imbalanced Treg/Th17 ratio accelerates the progression aGVHD. The aryl hydrocarbon receptor (AhR) basic helix-loop-helix transcription factor activated through cognate ligand binding. Current supports AhR plays critical regulatory role in differentiation Treg and Th17 cells. However, relationship...

p53 suppresses inflammatory and autoimmune diseases by inhibiting the inappropriate production of pro-inflammatory factors such as IL-1, IL-6, IL-12, TNF. In addition, plays an important role in regulating Th17/Treg balance. Accumulating evidence suggests that imbalance Th17 cells Tregs is cause aGVHD. However, whether abnormal expression participates development aGVHD remains unclear. this study, we demonstrated was downregulated CD4+ T from patients with compared non-aGVHD group....

Kinesin-12 (also named Kif15) participates in important events during neuronal development, such as cell division of precursors, migration young neurons and establishment axons dendritic arbors, by regulating microtubule organization. Little is known about the molecular mechanisms behind functions kinesin-12, even less its roles other types nervous system. Here, we show that kinesin-12 depletion from cultured rat cortical astrocytes decreases proliferation but increases migration....

Ureaplasma urealyticum (UU) is one of the most commonly occurring pathogens associated with genital tract infections in infertile males, but impact seminal UU infection semen on intrauterine insemination (IUI) outcomes poorly understood. We collected data from 245 couples who underwent IUI at The First Affiliated Hospital USTC (Hefei, China) between January 2021 and 2023. subjects were classified into two groups according to their status: UU-positive group UU-negative group. compared...

Abstract Background With the development of socio‐economic conditions and a shift in attitudes towards fertility, there has been gradual increase delayed childbearing since 2000s. Age plays significant role decline fertility. However, we know very little about association paternal age with reproductive outcomes. Objectives To investigate correlation between advanced semen quality, embryo pregnancy, neonatal outcomes IVF cycles. Materials methods In this study, after excluding female partners...

(UU) is a common pathogen associated with genital tract infections in infertile males. However, its impact on semen quality, embryo development, and